Mitochondrial Disruption Is Involved in the Effect of Nuclear Factor of Activated T cells, Cytoplasmic 4 on Aggravating Cardiomyocyte Hypertrophy

Abstract

Nuclear factor of activated T cells, cytoplasmic 4 (NFATc4), a nuclear transcription factor, has been implicated in cardiac hypertrophy through the enhancement of hypertrophic gene expression. However, the role of NFATc4 in mitochondrial modulation is mostly unknown. The current study aimed to investigate the role of NFATc4 in regulating mitochondrial function during phenylephrine (PE)-induced cardiac hypertrophy. Our results showed that overexpression of NFATc4 aggravated the PE-induced decrease in mitochondrial genesis, membrane potential, and mitochondrial gene expression as well as impaired mitochondrial respiration. However, knockdown of NFATc4 relieved PE-induced perturbations in mitochondria and cardiomyocyte hypertrophy. Mechanistically, by activating phosphoinositide-dependent kinase 1 and promoting a combination of AKT and phosphoinositide-dependent kinase 1, phosphorylation and sequential acetylation of PGC-1a were aggravated by NFATc4 and suppressed the activity of PGC-1a. In conclusion, NFATc4-regulated factors were shown to be associated with mitochondrial function and exacerbated PE-induced mitochondrial dysfunction. These findings revealed new roles of NFATc4 in cardiac hypertrophy.

Mitochondria in Pathological Cardiac Hypertrophy Research and Therapy

“…Nuclear factor of activated T cells, cytoplasmic 4 (NFATc4) overexpression aggravated phenylephrine (PE)-induced perturbations in mitochondrial genesis, membrane potential, and mitochondrial respiration; in contrast, NFATc4 deletion mitigated PE-induced mitochondrial dysfunction as well as cardiac hypertrophy ( 67 ). Mitochondrial dysfunction induced by Nlrp3 inflammasome also appears to play a regulatory role in Ang II-induced cardiomyopathy.…”