Mitochondrial DNA alterations in blood of the humans exposed to N,N-dimethylformamide

Shieh, Dar‐Bin; Chen, Chia Chun; Shih, Tung‐Sheng; Tai, Huo-Mu; Wei, Yau‐Huei; Chang, Ho-Yuan

doi:10.1016/j.cbi.2006.12.008

Cited by 29 publications

(16 citation statements)

References 43 publications

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“…[17][18][19] Long reactions times of several days are commonly used with slow heating and cooling rates using solvents such as N,N'-dimethylformamide (DMF), which is a toxic mutagen and environmentally hazardous. 20,21 Slow hydrothermal syntheses are typically carried out at temperatures up to 220 °C, while microwave, 22,23 sonication and mechanochemical 24,25 syntheses involve shorter reaction times in some cases. However, for microwave and alternative methods of synthesis to be cost effective for scale-up, they need to have clear benefits over conventional heating routes.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of metal–organic frameworks by continuous flow

et al. 2014

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A continuous flow process for the synthesis of a metal-organic framework using only water as the reaction medium and requiring only short residence times is described. This affords a new route to scale-up of materials incorporating many of the principles of green chemistry.The process is demonstrated by the synthesis MIL-53(Al) via continuous flow reaction requiring only 5-6 minutes with a space time yield of 1300 kg m -3 d -1 . We have demonstrated the synthesis of 500 g of MIL-53(Al) using this process, which can be scaled-up further by simply feeding further solutions of metal salt and ligand through the reactor. The product has a higher surface area and a better colour than a commercially produced sample of this MOF.In addition, a new and effective method for the extraction of terephthalic acid from within the pores of MIL-53(Al) using supercritical ethanol has been developed, representing a new methodology for activation and removal of substrates from porous hosts.___________________________________________________________________________

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Section: Introductionmentioning

confidence: 99%

Synthesis of metal–organic frameworks by continuous flow

et al. 2014

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“…In-vitro and in-vivo studies have identified generation of reactive oxygen species (ROS) and increased oxidative stress as a primary biological process that may contribute to produce such a variety of diverse health effects [9-14]. Epidemiologic [1,15] and in-vivo studies [16-18] suggest that both PM mass and PM metal components contribute to ROS generation. Mitochondria are the major intracellular source and primary target of ROS, which are generated under normal conditions as by-products of aerobic metabolism in animal and human cells.…”

Section: Introductionmentioning

confidence: 99%

Airborne particulate matter and mitochondrial damage: a cross-sectional study

et al. 2010

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BackgroundOxidative stress generation is a primary mechanism mediating the effects of Particulate Matter (PM) on human health. Although mitochondria are both the major intracellular source and target of oxidative stress, the effect of PM on mitochondria has never been evaluated in exposed individuals.MethodsIn 63 male healthy steel workers from Brescia, Italy, studied between April and May 2006, we evaluated whether exposure to PM was associated with increased mitochondrial DNA copy number (MtDNAcn), an established marker of mitochondria damage and malfunctioning. Relative MtDNAcn (RMtDNAcn) was determined by real-time PCR in blood DNA obtained on the 1st (time 1) and 4th day (time 2) of the same work week. Individual exposures to PM10, PM1, coarse particles (PM10-PM1) and airborne metal components of PM10 (chromium, lead, arsenic, nickel, manganese) were estimated based on measurements in the 11 work areas and time spent by the study subjects in each area.ResultsRMtDNAcn was higher on the 4th day (mean = 1.31; 95%CI = 1.22 to 1.40) than on the 1st day of the work week (mean = 1.09; 95%CI = 1.00 to 1.17). PM exposure was positively associated with RMtDNAcn on either the 4th (PM10: β = 0.06, 95%CI = -0.06 to 0.17; PM1: β = 0.08, 95%CI = -0.08 to 0.23; coarse: β = 0.06, 95%CI = -0.06 to 0.17) or the 1st day (PM10: β = 0.18, 95%CI = 0.09 to 0.26; PM1: β = 0.23, 95%CI = 0.11 to 0.35; coarse: β = 0.17, 95%CI = 0.09 to 0.26). Metal concentrations were not associated with RMtDNAcn.ConclusionsPM exposure is associated with damaged mitochondria, as reflected in increased MtDNAcn. Damaged mitochondria may intensify oxidative-stress production and effects.

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“…It is toxic to human beings and other organisms (with hepatotoxicity, embryotoxicity, teratogenicity, and possible carcinogenicity). Long-term exposure to DMF might also cause irreversible alterations of mitochondrial DNA (37). As the overall rate of chemical degradation of DMF is extremely slow, biodegradation might represent a viable alternative for the removal of this compound from industrial effluents and DMF-polluted sites.…”

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confidence: 99%

Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 Carries N , N -Dimethylformamide Degradation-Related Genes Whose Expression Is Activated by a LuxR Family Regulator

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Baj

et al. 2010

Appl Environ Microbiol

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N,N-Dimethylformamide (DMF), a toxic solvent used in the chemical industry, is frequently present in industrial wastes. Plasmid pAMI2 (18.6 kb) of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for methylotrophic growth of this bacterium, using DMF as the sole source of carbon and energy. Besides a conserved backbone related to pAgK84 of Agrobacterium radiobacter K84, pAMI2 carries a three-gene cluster coding for the protein DmfR, which has sequence similarities to members of the LuxR family of transcription regulators, and two subunits (DmfA1 and DmfA2) of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of DMF. Genetic analysis revealed that these genes, which are all placed in the same orientation, constitute an inducible operon whose expression is activated in the presence of DMF by the positive transcription regulator DmfR. This operon was used to construct a strain able to degrade DMF at high concentrations that might be used in the biotreatment of DMF-containing industrial wastewaters. To our knowledge, this is the first study to provide insights into the genetic organization and regulation as well as the dissemination in bacteria of genes involved in the enzymatic breakdown of DMF.

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Mitochondrial DNA alterations in blood of the humans exposed to N,N-dimethylformamide

Cited by 29 publications

References 43 publications

Synthesis of metal–organic frameworks by continuous flow

Synthesis of metal–organic frameworks by continuous flow

Airborne particulate matter and mitochondrial damage: a cross-sectional study

Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 Carries N , N -Dimethylformamide Degradation-Related Genes Whose Expression Is Activated by a LuxR Family Regulator

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