Mitochondrial DNA based diversity studies reveal distinct and sub-structured populations of pearlspot, Etroplus suratensis (Bloch, 1790) in Indian waters

Balasubramaniam, Sivamani; Soman, Misha; Katneni, Vinaya Kumar; Tomy, Sherly; Gopikrishna, G.; Vijayan, K.K.

doi:10.1007/s12041-021-01341-y

Cited by 6 publications

(8 citation statements)

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“…fimbriatus (Swain et al, 2014), L. calbasu (Singh et al, 2016), L. rohita (Luhariya et al, 2012), and Chitala chitala (Mandal et al, 2012). The low percentage of GC content and the least representation of nucleotide G (12.50%) were reported in other fish species as well (Swain et al, 2014;Sahoo et al, 2019), and these might be due to anti-G bias, which is characteristic for the mitochondrial genome (Kathirvelpandian et al, 2014;Balasubramaniam et al, 2022). In the present study, the D-loop region generated 58 haplotypes, with the Ganga River population having the highest number of haplotypes ( 24) followed by the river Brahmaputra ( 16), whereas the ATPase 6/8 region generated 17…”

Section: Discussionmentioning

confidence: 94%

“…information on genetic diversity, population structure, and evolutionary relationships of L. gonius within and among populations from the selected five major rivers of India based on the mitochondrial D-loop and ATPase 6/8 analysis (Paixão et al, 2018;Sahoo et al, 2020;Balasubramaniam et al, 2022). Since mitochondrial genes have a high rate of evolution, it allows for distinguishing between haplotypes in small sample sizes and threatened species (Habib et al, 2012;Das et al, 2024).…”

Section: Discussionmentioning

confidence: 99%

“…The D-loop control region is the marker of choice and has important value for investigating intraspecific genetic differentiation (Mandal et al, 2012;Sahoo et al, 2020;Zhang et al, 2020), and it has been the most widely used mtDNA marker in many phylogenetic genetic studies in fish (Xie et al, 2006;Paixão et al, 2018;Jiang et al, 2019;Gao et al, 2020;Sahoo et al, 2020). In addition, another important mitochondrial region ATPase 6/8 also mutates at a faster rate compared with the nuclear genes and is a potential marker analyzed for both phylogeny and phylogeography; hence, it is very useful in genetic diversity, population genetics, and evolution studies (Divya et al, 2015;Singh et al, 2016;Balasubramaniam et al, 2022). The ATPase 6/8 gene has been used successfully in many fish species (Behera et al, 2015(Behera et al, , 2018a(Behera et al, , 2018bMandal et al, 2012;Paixão et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of minor carp (Labeo gonius) from Indian rivers revealed through mitochondrial ATPase 6/8 and D-loop region analysis: implications for conservation and management

Roy,

Behera,

V. L.

et al. 2024

Front. Mar. Sci.

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India is the habitat of many freshwater fish species that make a significant contribution to endemism. However, the native species population is declining at a very fast rate due to pollution, habitat loss or degradation, new species introduction, hydrologic alterations, and overfishing. The minor carp, Labeo gonius, is one of the important fish species in the Indian river systems and holds enormous potential in culture fisheries. Therefore, the precise status of population structure is a prerequisite for the management of the species. In the present study, we assessed the potential of mtDNA ATPase 6/8 and D-loop regions as markers to determine the genetic diversity and phylogeography of L. gonius from Indian rivers. We have selected five major Indian rivers for investigation which could represent geographically distant populations of the studied fish. A total of 210 ATPase 6/8 sequences (842 bp) and 203 D-loop region sequences (683 bp) of L. gonius from the five major Indian rivers were submitted to NCBI. Results revealed that ATPase 6/8 has 17 haplotypes and 70 polymorphic sites with an overall haplotype diversity of 0.560 ± 0.036 and nucleotide diversity of 0.00347 ± 0.00. Furthermore, D-loop analysis revealed 58 haplotypes, 67 polymorphic sites, 0.954 ± 0.006 overall haplotype diversity, and 0.01914 ± 0.00 nucleotide diversity. The hierarchical AMOVA analysis indicated that a high proportion of the genetic variation (76.46% and 70.22% for the D-loop and ATPase 6/8 regions, respectively) was attributed to among-population variation under gene pool-1 (considering all the stocks from Ganga, Brahmaputra, Teesta, Narmada, and Mahanadi as a single pool). Furthermore, in gene pool-2, samples were divided into two groups: group 1 (Himalayan rivers)—Ganga, Brahmaputra, and Teesta; and group 2 (peninsular rivers)—Narmada and Mahanadi. Genetic variations in the D-loop and ATPase 6/8 regions were found among populations within groups at 70.65% and 53.48%, respectively. Haplotype network analysis revealed that there were significant differences in distributions and frequencies. The observed unique haplotypes might be useful for differentiating the stocks. In brief, the present study suggested that the D-loop and ATPase 6/8 regions are potential markers and represent a significant contribution to the genetic characterization of the minor carp L. gonius from major Indian rivers. Moreover, the L. gonius population along the east coast especially in the Himalayan rivers (Ganga, Brahmaputra, and Teesta river basin) is found to be genetically more diverse. The results would provide a scientific basis for the assessment of fisheries resources and management of this important species.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of minor carp (Labeo gonius) from Indian rivers revealed through mitochondrial ATPase 6/8 and D-loop region analysis: implications for conservation and management

Roy,

Behera,

V. L.

et al. 2024

Front. Mar. Sci.

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“…The demand for pearlspot consistently surpassed production and commands a higher market price (Balasubramaniam et al, 2022). The fish farmers are unable to capitalize on this due to limitations in the availability of quality seeds.…”

Section: Introductionmentioning

confidence: 99%

“…The demand for pearlspot consistently surpassed production and commands a higher market price (Balasubramaniam et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Integrating indoor and outdoor breeding systems to achieve year‐round seed production in Pearlspot cichlid, Etroplus suratensis

Varghese

George

Thiruvarasu

2022

Aquaculture Research

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Pearlspot, Etroplus suratensis naturally thrives in the estuarine reaches of Southern peninsular India and Srilanka. The relative ease of adaptation to diverse habitats makes it a promising candidate for climate-resilient aquaculture. This potential remains underexplored due to limitations in the current seed production practices. The present study focused on sustainable seed production of this species in the freshwater environment. Brooders were simultaneously maintained inside the hatchery and also in outdoor cement tanks. The breeding tanks were devoid of bottom substrate and planter pots were kept for egg attachment. The indoor fibreglass tank had an internal airlift filtration and outdoor tanks had an external filter connected to the filter bed.The modified captive breeding approaches employed were successful in obtaining 178 spawning from 44 pairs in 2 years. The indoor facility yielded 56 spawning from 15 pairs and the outdoor system yielded 122 spawning from 29 pairs. Pearlspot was found to spawn throughout the year with two distinct peaks, a minor peak in July-August and a major peak during December-January. Subsequently, spawning declined from February to June with an intermittent spurt in March. The peak spawning period distinctly differed between the breeding systems employed. The interval between successive spawning ranged from 10 to 308 days, with 46% of repetitive spawning occurring within 10 to 30 days. Pairs exhibited parental care by cleaning the substratum, protecting territory, caring for eggs and young ones. The clutch size varied from 347 to 2548, depending on the size, spawning frequency and inter-spawning interval.

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High Genetic Differentiation and Genetic Diversity in Endangered Mahseer Tor khudree (Sykes, 1839) as Revealed from Concatenated ATPase 6/8 and Cyt b Mitochondrial Genes

Das,

Sahoo

et al. 2024

Biochem Genet

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Mitochondrial DNA based diversity studies reveal distinct and sub-structured populations of pearlspot, Etroplus suratensis (Bloch, 1790) in Indian waters

Cited by 6 publications

References 53 publications

Genetic characterization of minor carp (Labeo gonius) from Indian rivers revealed through mitochondrial ATPase 6/8 and D-loop region analysis: implications for conservation and management

Genetic characterization of minor carp (Labeo gonius) from Indian rivers revealed through mitochondrial ATPase 6/8 and D-loop region analysis: implications for conservation and management

Integrating indoor and outdoor breeding systems to achieve year‐round seed production in Pearlspot cichlid, Etroplus suratensis

High Genetic Differentiation and Genetic Diversity in Endangered Mahseer Tor khudree (Sykes, 1839) as Revealed from Concatenated ATPase 6/8 and Cyt b Mitochondrial Genes

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