Mitochondrial DNA-based identification of some forensically important blowflies in Thailand

Preativatanyou, Kanok; Sirisup, Nantana; Payungporn, Sunchai; Poovorawan, Yong; Thavara, Usavadee; Tawatsin, Apiwat; Sungpradit, Sivapong; Siriyasatien, Padet

doi:10.1016/j.forsciint.2010.04.043

Cited by 17 publications

(12 citation statements)

References 12 publications

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“…Adult flies can carry intestinal worm eggs viz. Ascaris sp., hookworm, Trichuris trichura, Oxyiuris vermicularis, Taenia solium, Taenia saginata, Mycobacterium tuberculosis which causes pathogenicity in human (Preativatanyou et al, 2010;Sembel, 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Rain

Howlader

Aslam

2019

APJMBB

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Flies are considered serious pests which cause health problems of human and animal, transmitting many pathogenic microbes. Pest management programs depend on proper identification of pests. The present research work is an initiative to identify the medically and veterinary important flies based on mitochondrial COI gene sequences. Eleven species of the fly pests were identified. Among them, four fly species were the first record from Bangladesh. The phylogenetic analysis of retrieved sequences confirmed that the evolution of these species occurred from a common ancestor. Highest AT percentage (69.9%) was found in Haematobia irritans exigua and lowest GC percentage (30.4%) was found in Haematobia irritans exigua. The substitution rate of codon was found 1.88 in 1st position, 0.73 in 2nd position and 1.22 in 3rd position, respectively. Interspecific genetic divergence range of flies sequences was 5-20%. Haplotype network showed that Atylotus agrestis was mostly diverged from its common ancestors by 37 mutational steps. This research is the first molecular approach to identify the medically and veterinary important flies based on MT-COI gene sequences along with the establishment of first DNA barcode dataset for accurate identification in Bangladesh.

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Section: Introductionmentioning

confidence: 99%

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Rain

Howlader

Aslam

2019

APJMBB

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“…The COI gene has been widely studied in this species, and 28 COI sequences of this species from the NCBI GenBank (Table 3) are highly homologous to conspecific sequences in this study (average distance = 0.2%) [19, 20]. …”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences

Kim

Shin

Ham

et al. 2014

The Scientific World Journal

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Identification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed very low intraspecific sequence distances and species-level monophylies. However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation. To the best of our knowledge, this study is the first report of COI nucleotide sequences from Hydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, and Phaonia aureola.

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“…W analizach taksonomicznych i identyfikacyjnych najczęściej wykorzystuje się cechy zewnętrzne lub wewnętrzne organizmów [8]. Nieprawidłowo zabezpieczone próbki, a także duże podobieństwo większości preimaginalnych stadiów rozwojowych (jaj, larw, poczwarek) i fragmentów owadów mogą jednak utrudniać identyfikację [17][18][19][20][21][22]. Mimo zwiększonej dokładności identyfikacji gatunków na poziomie morfologicznym dzięki analizom wykonywanym za pomocą skaningowego mikroskopu elektronowego (SEM) podobne cechy stwierdzano u gatunków należących do różnych rodzin: plujkowatych (Calliphoridae), ścierwicowatych (Sarcophagidae) i muchowatych (Muscidae) [23].…”

Section: Identyfikacja Morfologicznaunclassified

“…The external or internal features of an organism are most utilized process in both taxonomy and identification [8]. However, in morphology-based approaches, badly preserved samples, the similarity of most immature stages (such eggs, larvae or pupae) or insect fragments are considered the major challenges in species identification [17][18][19][20][21][22]. In spite of the improve accuracy in identification of insect species on morphological level via scanning electron microscope (SEM), alike features were found in species belonging to Calliphoridae, Sarcophagidae and Muscidae [23].…”

Section: Morphological Identificationmentioning

confidence: 99%

Applications of forensic entomology: overview and update

Aly

Aldeyarbi

2020

amsik

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Streszczenie Mimo ogromnych postępów w badaniach podstawowych i stosowanych entomologia sądowa stale się rozwija i jest uznawana za interdyscyplinarną dziedzinę wiedzy. W zestawieniu z innymi naukami prawnymi i biologicznymi publikacje z zakresu entomologii sądowej są jednak stosunkowo skromne, podobnie jak liczba entomologów sądowych. Głównym celem niniejszej pracy jest przedstawienie znaczenia entomologii sądowej przez omówienie jej zastosowań, czynności podejmowane przez entomologów mogą bowiem dostarczać istotnych informacji dotyczących czasu, miejsca i przyczyny śmierci, ułatwiać identyfikację sprawców i/lub ofiar przestępstwa, a w niektórych przypadkach także określać stan zaniedbania ofiar przed śmiercią. W pracy omówiono też możliwość wykorzystywania zdobyczy najnowocześniejszych technologii. Interdyscyplinarność prac badawczych umożliwi dalsze zgłębianie tej dziedziny, przyczyniając się do wytyczania nowych obszarów badań z korzyścią dla rozwoju nauki oraz zastosowań kryminalistycznych.

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Mitochondrial DNA-based identification of some forensically important blowflies in Thailand

Cited by 17 publications

References 12 publications

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences

Applications of forensic entomology: overview and update

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