Mitochondrial DNA of chloramphenicol-resistant mouse cells contains a single nucleotide change in the region encoding the 3' end of the large ribosomal RNA.

Blanc, Hugues; Wright, Catharine T.; Bibb, Maureen J.; Wallace, Douglas C.; Clayton, David A.

doi:10.1073/pnas.78.6.3789

Cited by 97 publications

(43 citation statements)

References 30 publications

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“…The four mutation sites detected in this study differ from the other six sites which have been reported in mammalian CAP-R mutants (Fig. 7) (Blanc et al, 1981a and1981b;Kearsey and Craig, 1981;Koike et al, 1983;Slott et al, 1983;Howell and Lee, 1989;Howell and Kubacka, 1993;Hashiguchi and Ikushima, 1998). It is suggested that mutation sites outside the domain may be also implicated in binding of chloramphenicol, and thus in producing the CAP-R phenotype.…”

Section: Discussioncontrasting

confidence: 50%

“…The result suggests the likelihood that the six CAP-R mutants were clonal expansions of a very rare genotype that was present at low frequency in the V79 cells prior to CAP-R selection, though they were independently isolated from the different cultures. All of the four mutations may not be indispensable for the CAP-R phenotype, but any one of them may be enough to express CAP-R phenotype, because single mutation sites have been identified in mammalian CAP-R mutants (Blanc et al, 1981a and1981b;Howell and Kubacka, 1993). The CAP-R mutants isolated in this study are much more resistant to chloramphenicol than the known Chinese hamster CAP-R mutants (Howell and Kubacka, 1993).…”

Section: Discussionmentioning

confidence: 85%

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Novel point mutations in mitochondrial 16S rRNA gene of Chinese hamster cells.

Hashiguchi

Ikushima

2000

Genes Genet. Syst.

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To know the nature and mechanisms of spontaneous mutations in mitochondrial DNA (mtDNA), we determined, by direct cycle sequencing, the nucleotide sequence of the 3' terminal region of the mitochondrial 16S rRNA gene from chloramphenicol-resistant (CAP-R) mutants isolated in Chinese hamster V79 cells. Four different base substitutions were identified in common for the six CAP-R mutants. All mutations were heteroplasmic. One A to G transition was mapped at a site within the putative peptidyl transferase domain, the target region for chloramphenicol, and one G to A transition and two T to G transversions were located within the two different segments which form the stems of the hairpin loop structures attached to this key domain in the predicted secondary structure of 16S rRNA. The mutations detected in this study do not map to the same sites where CAP-R mutations were found previously in mammalian cells. Allele specific-PCR analyses revealed that all four mutations occurred on a single mutant-DNA molecule, but not on several ones independently. Together with the other previous reports, our data suggest that spontaneous mtDNA mutations may not be caused exclusively by oxidative DNA damage at least in 16S rRNA gene.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 85%

Novel point mutations in mitochondrial 16S rRNA gene of Chinese hamster cells.

Hashiguchi

Ikushima

2000

Genes Genet. Syst.

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“…Oligomycin resistance in S. cerevisiae can also be conferred by a mutation in the nuclear DNA, although no increase in resistance of the mitochondrial ATPase has been observed (2,13,40,45 (9,51), antimycin (24), rutamycin (33), erythromycin (19), and oligomycin (7,27), and mutants with defects in mitochondrial protein synthesis (52). Chloramphenicol resistance has recently been shown to be due to a mutation in the 16S rRNA gene of the mitochondrial DNA (4,29). Cells bearing nuclear mutations affecting mitochondrial functions have also been isolated, including respirationdeficient cells (17,48) with defects in NADHcoenzyme Q reductase activity (6,16), succinate dehydrogenase activity (47), and mitochondrial protein synthesis (10,18).…”

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confidence: 99%

Genetics of the mammalian oxidative phosphorylation system: characterization of a new oligomycin-resistant Chinese hamster ovary cell line.

Breen¹

1982

Mol. Cell. Biol.

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“…The relationship between chloramphenicol resistance and mtDNA was then firmly established when the sequence of the 3' end of the 16S rRNA gene in CAPr mutants was compared with that observed in normal mouse and human mtDNA. In each of five CAPr mutants studied (4,5,12), a single base substitution was found within one of two, highly conserved, 10-t base pair segments. Substitutions in these regions were predicted because Dujon (9) discovered substitutions in homologous sequences within the large rRNA genes of two CAP' mutants of Saccharomyces cerevisiae.…”

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confidence: 99%

Sequence analysis of mitochondrial DNA in a mouse cell line resistant to chloramphenicol and oligomycin.

Slott¹,

Shade²,

Lansman³

1983

Mol. Cell. Biol.

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A mouse L-cell line, designated 111-OB3, is described which is resistant to two drugs, chloramphenicol and oligomycin. The cells contain two types of mitochondrial DNA molecules, in roughly equal proportions, which differ in that one is cleaved by endonuclease EcoRI at a novel site within the coding sequence for subunit 6 of the mitochondrial ATPase (ATPase-6). Sequence analysis reveals that the cleavage site was created by a single transversion which predicts a replacement of valine in the wild-type ATPase-6 by glutamic acid. The replacement occurs in a hydrophobic amino acid sequence which is highly conserved in mouse, human, and bovine proteins. The position of the replacement is similar to a substitution observed in one class of yeast mutants resistant to oligomycin. Both of the mitochondrial DNA molecules in 111-OB3 also have a single nucleotide change in the gene encoding the large (16S) rRNA. These observations are consistent with the hypothesis that oligomycin resistance in mammalian cells can be cytoplasmically determined and can result from alterations in ATPase-6. The appearance of the mutation before selection in oligomycin suggests a model for the origin of mitochondrial mutations in mammalian cells.

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Mitochondrial DNA of chloramphenicol-resistant mouse cells contains a single nucleotide change in the region encoding the 3' end of the large ribosomal RNA.

Cited by 97 publications

References 30 publications

Novel point mutations in mitochondrial 16S rRNA gene of Chinese hamster cells.

Novel point mutations in mitochondrial 16S rRNA gene of Chinese hamster cells.

Genetics of the mammalian oxidative phosphorylation system: characterization of a new oligomycin-resistant Chinese hamster ovary cell line.

Sequence analysis of mitochondrial DNA in a mouse cell line resistant to chloramphenicol and oligomycin.

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