Mitochondrial DNA Polymorphism in Candida albicans

Pd, Olivo; Ej, McManus; Ws, Riggsby; Jm, Jones

doi:10.1093/infdis/156.1.214

Cited by 56 publications

(29 citation statements)

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“…DNA length polymorphisms have also been reported for C. albicans mitochondrial DNA by Olivo et al (78). Wholecell DNA, prepared by producing spheroplasts, followed by lysing, was digested with restriction enzymes, and the digests were electrophoresed in agarose or polyacrylamide.…”

Section: Electrophoretic Karyotypingmentioning

confidence: 79%

Candida albicans strain delineation

Merz

1990

Clin Microbiol Rev

118

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Candida albicans is a major opportunistic pathogen causing a wide spectrum of disease in human beings. Methods for strain delineation of this species to assess or predict virulence or to conduct epidemiologic or pathogenetic investigations have been developed. Although factors associated with virulence have been identified, there is no rapid system to quantitate them in a clinical laboratory. Therefore, many typing methods are based on variable phenotypic characteristics within this species including morphotyping, serotyping, antibiogram, resistogram typing, biotyping, biotyping based on commercial carbon assimilation patterns, enzyme profiles, sensitivity to yeast killer toxins, and typing based on protein variability. Phenotypically defined strains generally do not correlate with the pathogenic potential of a strain with the exception of morphotyping. However, these methods can be useful in epidemiologic investigations; for example, they have revealed that most individuals harbor one strain and that infections are frequently due to an endogenous strain. Problems with these methods usually relate to their discriminatory power. When this is maximized, reproducibility (especially between laboratories) suffers. Recently, methods based on differences in DNA structure (genotyping) for strain delineation have been developed, including electrophoretic karyotyping and restriction enzyme fragment length polymorphisms. The development of a computer-assisted data bank and analysis for these genotypic strain delineators will open investigations into the pathogenesis of this infection and permit epidemiologic studies previously not possible with this important human pathogen.

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Section: Electrophoretic Karyotypingmentioning

confidence: 79%

Candida albicans strain delineation

Merz

1990

Clin Microbiol Rev

118

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“…Fungi also contain multiple copies of the mitochondrial genome (see, e.g., reference 373). rDNA sequences (326) and to a lesser extent mitochondrial DNA sequences (251,427) represent the majority of intense bands in an RFLP pattern. A particularly good ethidium bromide-stained gel containing RFLP patterns of a number of C. albicans isolates is presented in Fig.…”

Section: Restriction Fragment Length Polymorphism Without Hybridizationmentioning

confidence: 99%

“…Wills et al (427) obtained five distinct bands, and the pattern varied among isolates sufficiently to suggest that Southern blot hybridization with a mitochondrial DNA probe would be effective in identifying the same strain in independent isolates and in distinguishing among unrelated strains of C. albicans (251). In an analysis of type I and type II Candida stellatoidea, a close relative of C. albicans, the hybridization patterns of type I isolates were identical but those of type II isolates varied (170).…”

Section: Restriction Fragment Length Polymorphisms With Hybridizationmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Soll

2000

Clinical Microbiology Reviews

283

317

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“…A physical map of the mitochondrial genome of C. albieans has been constructed wherein six EeoRI fragments have been cloned [123]. Givo et al analyzed a variety of C. albieans isolates with these EeoRI fragments [82] and found a restriction pattern of polymorphism in the mtDNA of C. albieans. mtDNA polymorphism has also been seen in a number of other eukaryotic organisms [14,39,97].…”

Section: Mitochondrial Dnamentioning

confidence: 99%