Mitochondrial DNA single nucleotide polymorphism associated with weight estimated breeding values in Nelore cattle (Bos indicus)

Biase, Fernando H.; Meirelles, Flávio Vieira; Gunski, Ricardo José; Vozzi, Pedro Alejandro; Bezerra, Luiz Antônio Framartino; Vila, Reginaldo Aparecido; Rosa, A. do N.; Lôbo, Raysildo Barbosa; Martelli, Lúcia Regina

doi:10.1590/s1415-47572007000600005

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…As mtDNA haplotypes have been suggested to influence milk [ 66 ] and meat [ 67 ] quality, and estimated breeding values [ 68 ] in cattle, growth and physical performance in mice [ 69 ], fertility in beef cattle [ 70 ] and pigs [ 42 ], development to term of cattle produced by somatic cell nuclear transfer [ 32 ] and expression levels of heat shock proteins in human cells [ 19 ], the combination of knowledge about mtDNA, assisted reproductive technologies and breeding strategies could lead to the generation of prime founder animals with the desired genotypes that could then be dispersed and maintained through the breeding industry. It has long been debated whether somatic cell nuclear transfer and other more sophisticated assisted reproductive technologies could be employed to generate super breeds of cattle and, specifically, new lineages of cattle (reviewed by [ 18 , 52 ]).…”

Section: Discussionmentioning

confidence: 99%

Cattle phenotypes can disguise their maternal ancestry

et al. 2017

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BackgroundCattle are bred for, amongst other factors, specific traits, including parasite resistance and adaptation to climate. However, the influence and inheritance of mitochondrial DNA (mtDNA) are not usually considered in breeding programmes. In this study, we analysed the mtDNA profiles of cattle from Victoria (VIC), southern Australia, which is a temperate climate, and the Northern Territory (NT), the northern part of Australia, which has a tropical climate, to determine if the mtDNA profiles of these cattle are indicative of breed and phenotype, and whether these profiles are appropriate for their environments.ResultsA phylogenetic tree of the full mtDNA sequences of different breeds of cattle, which were obtained from the NCBI database, showed that the mtDNA profiles of cattle do not always reflect their phenotype as some cattle with Bos taurus phenotypes had Bos indicus mtDNA, whilst some cattle with Bos indicus phenotypes had Bos taurus mtDNA. Using D-loop sequencing, we were able to contrast the phenotypes and mtDNA profiles from different species of cattle from the 2 distinct cattle breeding regions of Australia. We found that 67 of the 121 cattle with Bos indicus phenotypes from NT (55.4%) had Bos taurus mtDNA. In VIC, 92 of the 225 cattle with Bos taurus phenotypes (40.9%) possessed Bos indicus mtDNA. When focusing on oocytes from cattle with the Bos taurus phenotype in VIC, their respective oocytes with Bos indicus mtDNA had significantly lower levels of mtDNA copy number compared with oocytes possessing Bos taurus mtDNA (P < 0.01). However, embryos derived from oocytes with Bos indicus mtDNA had the same ability to develop to the blastocyst stage and the levels of mtDNA copy number in their blastocysts were similar to blastocysts derived from oocytes harbouring Bos taurus mtDNA. Nevertheless, oocytes originating from the Bos indicus phenotype exhibited lower developmental potential due to low mtDNA copy number when compared with oocytes from cattle with a Bos taurus phenotype.ConclusionsThe phenotype of cattle is not always related to their mtDNA profiles. MtDNA profiles should be considered for breeding programmes as they also influence phenotypic traits and reproductive capacity in terms of oocyte quality.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0523-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Cattle phenotypes can disguise their maternal ancestry

et al. 2017

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“…The results of the Bonferroni test were found to be significant (5%), and each animal that received the C allele tended to weigh on average 10.2 kg less than its contemporary. Several studies have investigated allelic profiles by SNP genotyping in cattle populations (Damiani et al, 2000;Flisikowski et al, 2005;de Souza et al, 2007;Andréa et al, 2007;Biase et al, 2007;Curi et al, 2009;Bagnicka et al, 2010;Souza et al, 2010). Up to 2009, around 500 bulls from 123 farms participating in the Genetic Improvement Program of the Nellore Breed in Brazil have been evaluated for the future multiplication of "positive animals" in commercial herds by artificial insemination, and of this total number, 25% were evaluated in the present study.…”

Section: Resultsmentioning

confidence: 99%

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

Martins-da-Silva¹,

Rios²,

Ramos³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We analyzed two single nucleotide polymorphisms (SNPs) of the IGF2 and CYP21 genes in Nellore cattle participating in the Brazilian Animal Breeding Program. The SNPs were found in exon 6 of the IGF2 (insulin-like growth factor 2) gene (RFLP/MboII) as well as in the promoter region of the CYP21 (steroid 21-hydroxylase) gene (RFLP/HpaII) of these animals. The TC heterozygotes were significantly more frequent than CC and TT homozygotes in the RFLP/ MboII polymorphism. The T allele was significantly more frequent than the C allele in RFLP/HpaII polymorphism. This population was found IGF2 and CYP21 gene polymorphisms in Nellore cattle to be in Hardy-Weinberg equilibrium for these SNPs. Association of these polymorphisms with expected progeny differences of reproductive and productive traits was investigated, but proved to be significant only for DP550 (expected progeny differenced for weight at 365 days -IGF2 -RFLP/MboII) and DP450 (expected progeny differenced for weight at 450 days -CYP21 -RFLP/HpaII). This is the first study on the occurrence of these two polymorphisms in this Zebu breed of cattle. A total of 147 Nellore animals participating in the Breeding Program of the Nellore Breed (PMGRN) under the management of the National Association of Breeders and Researchers (ANCP) in the city of Ribeirão Preto were analyzed.

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“…Mitochondrial haplotypes have been shown to predispose individuals to and protect them against several diseases and phenotypic traits. For example, mtDNA haplotypes have been suggested to in uence milk and meat quality and estimated breeding values in cattle, growth and physical performance in mice, fertility in beef cattle and pigs, development to the term of cattle produced by somatic cell nuclear transfer and expression levels of heat shock proteins in human cells [17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA D-loop A/G Transition at Position 869 as a Reproductive Performance Indicator in Gir Cattle

Malik,

E.M

2023

Preprint

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Reproduction is a critical aspect of livestock production, with mitochondria playing a pivotal role in providing the necessary energy for this high-demanding process. Maternally inherited mitochondria are known to influence various cellular functions, including ATP production and apoptotic regulation, and possess their own genome, including the Displacement loop (D-loop). This study aims to explore the mitochondrial DNA (mtDNA) D-loop SNPs and their potential association with reproductive traits in Gir cattle, an indigenous breed. Two groups of animals were categorized based on their reproductive performance, with good performers demonstrating shorter calving intervals, gestation lengths, days open, and fewer services per conception. Total DNA was isolated from blood samples, and mtDNA D-loop sequences were obtained through Sanger sequencing, resulting in 29 haplotypes. Comparative analysis revealed that animals with poor reproductive performance exhibited a higher number of polymorphic sites, transition/transversion biases, and occurrences of insertions/deletions (INDELs) compared to the good performers. Specifically, good performers showed higher A/G and G/A substitutions, while poor performers exhibited higher T/A, C/A, A/T, G/T, and G/C substitutions. The A/G transition at Position 869 was significantly associated with poor reproductive performance, impacting secondary structure stability and likely affecting energy efficiency. Validation of these findings in a larger Gir cattle population and exploring mtDNA polymorphisms in coding regions may establish a maternal lineage-based biomarker for assessing reproductive efficiency in this indigenous breed. This study sheds light on the significance of mitochondrial genetic factors in shaping reproductive traits and offers insights into sustainable livestock production.

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Mitochondrial DNA single nucleotide polymorphism associated with weight estimated breeding values in Nelore cattle (Bos indicus)

Cited by 7 publications

References 29 publications

Cattle phenotypes can disguise their maternal ancestry

Cattle phenotypes can disguise their maternal ancestry

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

Mitochondrial DNA D-loop A/G Transition at Position 869 as a Reproductive Performance Indicator in Gir Cattle

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