Mitochondrial DNA variation in indigenous sheep (Ovis aries) breeds of Nepal

Gorkhali, Neena Amatya; Han, Jianlin; H., Y.

doi:10.4038/tar.v26i4.8125

Cited by 14 publications

(9 citation statements)

References 25 publications

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“…This pattern is similar to that observed for the five Egyptian sheep breeds [37], Romanian Racha Sheep [49], and Iberian sheep [57] where Hap B is predominant over Hap A. However, this in contrast to the observations of the four Nepal indigenous sheep breeds [51], Tibetan sheep [26], and native Mongolian sheep [3], where Hap A is predominant over Hap B.…”

Section: Haplotype Analysissupporting

confidence: 86%

“…This is in line with the previous studies which states that several sheep breeds in Indonesia have high genetic diversity within [39] and between [50] populations. Unique patterns in the distribution of haplotypes could be attributed to the total reproductive isolation because of the harsh geographical structure of and unique husbandry practice on Java Island, that allow farmers to select animals with unique phenotypic characters associated with the adaptability to different ecological conditions [51,52]. Hap A is mainly represented in Asian breeds, whereas Hap B is found in high frequencies in European breeds [54,55].…”

Section: Haplotype Analysismentioning

confidence: 99%

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The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

et al. 2020

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Background and Aim: Java Island is one of the islands in Indonesia which has local sheep breeds with specific characteristics and native development geography in certain regions. This study aimed to determine the genetic profiles and maternal origin of six local sheep breeds on Java Island. Materials and Methods: This study was conducted by identifying the profiles of complete mitochondrial DNA (mtDNA) displacement loop (D-loop) region sequences on a total of 22 individual in six local sheep breeds on Java Island, including Javanese thin-tailed (JTT), Javanese Fat-Tailed (JFT), Batur (BTR), Wonosobo (WSB), Garut (GRT), and Priangan (PRG) sheep. The D-loop region was amplified using specific primers, and the polymerase chain reaction (PCR) was performed. The PCR products were purified and sequenced. Results: The mtDNA D-loop analysis identified 21 haplotypes in the analyzed 22 animals with 123 polymorphic sites (V) consisting of 60 singleton variable sites (S) and 63 parsimony informative sites (P). Within all breeds tested, the haplotype diversity, the average number of pairwise differences (K), and nucleotide diversity (Pi) were 0.99567, 25.36364, and 0.02153, respectively. The genetic distance (D) within groups and between groups was 0.001-0.006 and 0.004-0.036, respectively. The phylogeny resulted in the presence of two haplogroups (Hap), which are 5 Hap A and 16 Hap B. All JTT, JFT, BTR, and WSB breeds were in the same cluster in Hap B, whereas GRT and PRG breeds were in clusters in both Hap A and Hap B. Conclusion: The high genetic diversity in six local sheep breeds on Java Island suggests that they originated from different genetic sources. JTT sheep have closer genetic relationships to JFT, BTR, and WSB sheep, and they are close to European sheep, whereas GRT sheep have closer genetic relationships to PRG sheep. Both are closer to Asian sheep than to European sheep.

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Section: Haplotype Analysissupporting

confidence: 86%

Section: Haplotype Analysismentioning

confidence: 99%

The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

et al. 2020

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“…Studies of many scientists on sheep from the Egyptian and Italian sheep breeds (Othman et al, 2015), Iranian sheep (Rafia and Tarang, 2016), Polish sheep (Koseniuk and Slota, 2016), Nepalese sheep (Gorkhali et al, 2015) were presented. The fragment of the D-loop region used in these studies varied from 76 to 721 b.p., with 55-132 polymorphic sites.…”

Section: Resultsmentioning

confidence: 99%

Genetic Characterization of Kazakh Native Sheep Breeds Using Mitochondrial DNA

Mukhametzharova¹,

Islamov²,

Shauyenov³

et al. 2018

OnLine Journal of Biological Sciences

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In this study, we used mitochondrial (mt) DNA to reveal patterns of genetic diversity among six breeds of sheep native to Kazakhstan, including the Chu and Kalchengel types of Kazakh mutton-semifine-wool breed of sheep, Akzhayik, Edilbay, Kazakh fat-rumped semi-course-wool and South Kazakh Merino breeds. Mitochondrial DNA D-loop was studied in 64 samples to determine the genetic diversity of the local sheep breeds. The analysis identified 54 haplotypes and 113 polymorphic loci of mtDNA D-loop. For the studied breeds, haplotype diversity and nucleotide diversity were 0.993±0.005 and 0.039±0.001, respectively. The results indicated higher level of nucleotide diversity in the South Kazakh Merino (SKM) breed (0.047), while the lowest diversity was observed in the Edilbay breed (0.029). In addition, the average number of nucleotide differences (Kxy) and the average number of nucleotide substitutions (Dxy) were determined among the studied breeds. The minimal value of genetic distance was observed between Akzhayik mutton-wool and Kazakh fat-rumped semicourse-wool breeds (Kxy: 16.956 and Dxy: 0.039), while the maximum value was observed between the Edilbay and SKM breeds (Kxy: 24.600 and Dxy: 0.040). Phylogenetic analysis revealed the presence of three haplogroups, namely, A, B and C in the collected sample, while the haplogroups D and E were not found. The results indicated that 28 of 64 tested animals belong to haplogroup A (43.75%), whereas 25 of investigated animals were attributed to haplogroup B (39.06%) and 11 animals to haplogroup C (17.19%).

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“…Autohtoni genotipovi ovaca i koza se uglavnom gaje u brdsko-planinskom području, pretežno ekonomski nerazvijenih regiona sa skromnim izvorima hrane (Žujović i sar, 2011). Lokalno prilagođeni genotipovi predstavljaju značajan faktor održanja u pogledu ekonomskog opstanka, naročito u zemljama u razvoju, budući da su to genotipovi kombinovanih proizvodnih svojstava, te predstavljaju potencijalan izvor mesa, vune, mleka, stajnjaka i kože (Gorkhali et al, 2015). Ovi genotipovi su važan element regionalnog agro-biodiverziteta po značaju koji imaju na agro-ekosisteme koji određuju kulturno nasleđe datog regiona (Jovanović i sar., 2011).…”

Section: Uvodunclassified

Sheep and goat genetic resources in Central Serbia

Cekić¹,

Petrovic²,

Ružić-Muslić³

et al. 2018

Selekcija i semenarstvo

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Na teritoriji Centralne Srbije od lokalno prilagođenih rasa ovaca, prisutna je pramenka sa diferenciranim sojevima, a od koza balkanska i srpska bela koza. Ove rase se međusobno razlikuju po morfološkim, reproduktivnim i proizvodnim osobinama i na njihovo nastajanje su uticali specifični klimatski, hidrološki i ishrambeni uslovi, zahvaljujući kojima se one odlikuju dobrom adaptabilnošću, otpornošću na bolesti, izraženim materinskim instinktom, relativno dobrom plodnošću i dugim proizvodnim vekom. Produktivnost ovih genotipova je relativno niska zbog čega su u proizvodnji potencirani produktivniji genotipovi. To je dovelo do drastičnog smanjenja broja grla lokalno prilagođenih rasa, a samim tim i do gubitka genetičke raznovrsnosti. Kao posledica navedenog, većina lokalno adaptiranih rasa je prema važećem Pravilniku ugrožena u različitom stepenu (od visoko do kritično ugrožene). Na osnovu godišnjeg izveštaja Instituta za stočarstvo za 2017. godinu, brojno stanje kvalitetnih priplodnih grla većine lokalno prilagođenih rasa je u slabom porastu i dinamika porasta nije zadovoljavajuća. Tako, broj aktivnih grla upisanih u glavnu matičnu evidenciju ovaca i koza za 2017. godinu po rasama iznosi 10386 za svrljišku pramenku, lipska pramenka 686, krivovirska pramenka 616, karakačanska pramenka 139, pirotska pramenka 124, bardoka 94 grla, balkanska koza 121 grlo i srpska bela koza 145 grla. Kontrola produktivnih osobina je obavljena na ukupno 6186 grla kod tri genotipa ovaca (lipska, svrljiška i krivovirska pramenka) i 237 grla kod dva genotipa koza (balkanska i srpska bela). Najveće vrednosti telesnih masa jagnjadi utvrđene su kod lipske pramenke (TM na rođenju 3,44 kg; TM sa 30 dana 11,79 kg i TM na odlučenju 27,92 kg), a jaradi kod srpske bele koze (TM na rođenju 2,56, TM sa 30 dana 7,68 kg i TM na odlučenju 16,04 kg). Najveći indeksi plodnosti su utvrđeni kod grla svrljiške pramenke (1,28), odnosno balkanske koze (1,51). Zaštita genetičkih resursa u Republici Srbiji još uvek nije dovoljna, iako ima pomaka u njihovom očuvanju. Ipak, hitne mere zaštite se moraju preduzeti u vidu In-Situ i Ex-Situ konzervacije kako bi se sprečio gubitak njihovog genofonda.

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Mitochondrial DNA variation in indigenous sheep (Ovis aries) breeds of Nepal

Cited by 14 publications

References 25 publications

The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

Genetic Characterization of Kazakh Native Sheep Breeds Using Mitochondrial DNA

Sheep and goat genetic resources in Central Serbia

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