Mitochondrial dysfunction in resveratrol-induced apoptosis in QGY-7701 cells

Li, J P; Ma, Qing; Chen, C M

doi:10.4238/gmr.15017490

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…11 However, whether δ-TOCO possesses anticancer activity against CML has not yet been explored. Resveratrol (RES), a potent antioxidant, induced in QGY-7701 cells apoptosis by excessive production of ROS 12 and in T-cell Acute Lymphoblastic Leukemia cells inhibiting AKT/ mTOR/p7056K/4E-BP1pathways 13 as well as, in K562/ ADR cells, it exerts its antiproliferative activity by suppressing the PIK/Akt/mTOR signaling pathway. 14 Recently, Azhar et al 15 demonstrated that apoptotic activity of RES involved a suppression of AKT/PKB pathway via generation of ROS in large B cell lymphoma.…”

Section: Introductionmentioning

confidence: 99%

Effects of antioxidants on apoptosis induced by dasatinib and nilotinib in K562 cells

Damiano

Montagnaro²,

Puzio³

et al. 2018

J of Cellular Biochemistry

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In clinical practice for the treatment of chronic myeloid leukemia, second generation of tyrosine kinase inhibitors such as Nilotinib (NIL) specific and potent inhibitor of the BCR/ABL kinase and Dasatinib (DAS) a inhibitor of BCR/ABL and Src family kinase were developed to clinically overcome imatinib resistance. In this study, we wanted to test the ability of some antioxidants such Resveratrol (RES) or a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) or δ-tocotrienol (δ-TOCO) to interact with DAS and NIL on viability, reactive oxygen species (ROS) production, lipid peroxidation, and apoptosis. To test the possible mechanisms of action of such antioxidants, we utilized N-acetyl-L-cysteine (NAC) a specific inhibitor ROS production or PP1 a specific Src tyrosine kinase inhibitor or BAPTA a specific chelator of intracellular calcium. Our data demonstrated: 1) RES, rMnSOD, δ-TOCO, and NAC, at dose used, significantly reduced the intracellular levels of MDA induced by DAS or NIL; 2) RES, rMnSOD, and δ-TOCO increased the intracellular ROS levels; 3) The increase ROS levels is related to higher levels of oligonucleosomesi induced by DAS and NIL and that NAC significantly reduced this activity. Interestingly, our data showed that apoptotic activity of DAS and NIL have significantly increased the production of oligonucleosomes by triggering excessive ROS generation as well as functionality of SERCA receptors.

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