Mitochondrial filaments and clusters as intracellular power-transmitting cables

Vp, Skulachev

doi:10.1016/s0968-0004(00)01735-7

Cited by 412 publications

(337 citation statements)

References 35 publications

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“…63 Thus it is possible that the Arp2/3 complex subunit 4 identified here plays a role in cytoskeletal rearrangements and/or mitochondrial changes in the ultrastructural organization during the process of apoptosis. 64 Finally, we also found the type-I RNA helicase Upf1 as being released under influence of tBid. Upf1 is involved in regulation of translation by operating in the nonsensemediated decay of mRNA.…”

Section: Mit (Mom)mentioning

confidence: 57%

A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid

et al. 2002

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A crucial event in the process of apoptosis is caspasedependent generation of truncated Bid (tBid), inducing release of cytochrome c. In an in vitro reconstitution system we combined purified recombinant tBid with isolated liver mitochondria and identified the released proteins using a proteomic matrix-assisted laser desorption ionization postsource decay (MALDI-PSD) approach. In order to meet physiological conditions, the concentration of tBid was chosen such that it was unable to induce cytochrome c release in mitochondriaderivedfrom liver-specificBcl-2-transgenicmice. Several mitochondrial proteins were identified to be released in a tBid-dependent way, among which cytochrome c, DIABLO/ Smac, adenylate kinase 2, acyl-CoA-binding protein, endonuclease G, polypyrimidine tract-binding protein, a type-I RNA helicase, a WD-40 repeat-containing protein and the serine protease Omi. Western blotting confirmed the absence of adenylate kinase 3, a matrix mitochondrial protein. These results demonstrate that a physiologically relevant concentration of tBid is sufficient to induce release of particular intermembrane mitochondrial proteins belonging to a broad molecular-mass range.

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Section: Mit (Mom)mentioning

confidence: 57%

A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid

et al. 2002

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“…On one hand, mitochondrial fusion would either allow the complementation between different mitochondria that mix their matrix contents or the electric coupling of different mitochondria. On the other hand, fission would either generate small mitochondria that can be delivered to distant regions of the cell where energy is required, as the synaptic boutons in neuronal axons, or would allow the segregation of pre-existing mitochondria between daughter cells during mitosis (Nakada et al, 2001;Skulachev, 2001;Detmer and Chan, 2007;Twig et al, 2008a). Pioneering works from several laboratories have described some of the proteins involved in the fusion and fission events, amongst which the best characterized are: mitofusin1 and mitofusin 2 (Mfn1, Mfn2), that are involved in the fusion of the outer mitochondrial membrane; OPA1, that mediates mitochondrial inner membrane fusion; and Drp1, known to be essential for mitochondrial fission (reviewed in Chen and Chan, 2009;Sauvanet et al, 2010).…”

Section: Alterations In Mitochondrial Dynamicsmentioning

confidence: 99%

Mitochondrial respiratory chain dysfunction: Implications in neurodegeneration

Morán

Moreno-Lastres

Marín-Buera

et al. 2012

Free Radical Biology and Medicine

144

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For decades mitochondria have been considered static round shaped organelles in charge of energy production. On the contrary, they are highly dynamic cellular components that undergo continuous cycles of fusion and fission influenced, for instance, by oxidative stress, cellular energy requirements, or the cell cycle state. New important functions beyond energy production have been attributed to mitochondria, such as the regulation of cell survival due to their role in the modulation of apoptosis, autophagy and aging. Primary mitochondrial diseases due to mutations in genes involved in these new mitochondrial functions, and the implication of mitochondrial dysfunction in multifactorial human pathologies like cancer, Alzheimer's and Parkinson's diseases, or diabetes has been demonstrated. Therefore, mitochondria are set at a central point of the equilibrium between health and disease, and a better understanding of mitochondrial functions will open new fields to explore the role of these mitochondrial pathways in human pathologies. The present review will dissect the relationships between the activity and assembly defects of the mitochondrial respiratory chain, oxidative damage, and alterations in mitochondrial dynamics, with special focus at their implications in neurodegeneration.

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“…Each swelling contained six to nine mitochondria, of which four or five were interconnected. This arrangement could reflect individual mitochondria connected with each other (Brodin et al, 1999;Skulachev, 2001) or may result from the segmentation of a single mitochondrion (King et al, 1996;Rowland et al, 2000). Because the formation of tubular structures could be Figure 4.…”

Section: Ultrastructural Correlate Of Sv Donutsmentioning

confidence: 99%

“…These mitochondria were tightly packed and interconnected. Distinct subcellular localization and interconnection of mitochondria have been described in various cell types including neurons and may allow functional coupling (King et al, 1996; Skulachev, 2001;Hollenbeck, 2005). Boosting mitochondrial function by such coupling could affect several processes essential for synaptic transmission.…”

Section: A Central Cluster Of Interconnected Mitochondriamentioning

confidence: 99%

Donut-Like Topology of Synaptic Vesicles with a Central Cluster of Mitochondria Wrapped into Membrane Protrusions: A Novel Structure–Function Module of the Adult Calyx of Held

et al. 2006

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Structural and functional properties of synapses are intricately and reciprocally coupled. To cope with the functional requirements in auditory processing, the calyx of Held developed distinct structural specializations such as a large number of active zones, large size, elaborate morphology, and defined distribution of ion channels. These specializations typically appear during postnatal maturation within the first 3 weeks of life and are accompanied by marked changes in the properties of synaptic transmission. We examined the arrangement of synaptic vesicles at different postnatal stages of maturation by genetically labeling vesicles with the fluorescent fusion protein synaptophysin-enhanced green fluorescent protein. Fluorescence and electron microscopy-based analyses revealed a new anatomical specialization in the mature calyx of Held. Within small, membrane-delimited compartments (swellings), synaptic vesicles formed donut-like assemblies around a central cluster of interconnected mitochondria. Adult calyces contained ϳ100 such structural units, each of them consisting of ϳ800 synaptic vesicles, six to nine mitochondria, and five to nine active zones. A donut of synaptic vesicles measured ϳ1 m in diameter and was placed in a swelling with a volume of ϳ5 fl. Conspicuously, this structural specialization appears with the onset of hearing and may contribute to maturational changes in presynaptic function.

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Mitochondrial filaments and clusters as intracellular power-transmitting cables

Cited by 412 publications

References 35 publications

A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid

A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid

Mitochondrial respiratory chain dysfunction: Implications in neurodegeneration

Donut-Like Topology of Synaptic Vesicles with a Central Cluster of Mitochondria Wrapped into Membrane Protrusions: A Novel Structure–Function Module of the Adult Calyx of Held

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