Mitochondrial integrity during early reperfusion in an isolated rat heart model of donation after circulatory death—consequences of ischemic duration

Heart transplantation from donation after circulatory death donors: Present and future

“…In reanimation studies of heart, with shorter duration of WIT (10 minutes) the hearts regained full function but with WIT of over 30 minutes, heart function remains suboptimal 12 . Data from our group and others established a maximum WIT of 25 minutes for rat hearts beyond which hearts would not regain optimal function 14,15 . Ischemia duration of 20, 25, and 30 minutes lead to a decrease in mitochondrial function (rate of oxidative phosphorylation) by 33%, 38%, and 60%, respectively.…”

“…| 877 maximum WIT of 25 minutes for rat hearts beyond which hearts would not regain optimal function. 14,15 Ischemia duration of 20, 25, and 30 minutes lead to a decrease in mitochondrial function (rate of oxidative phosphorylation) by 33%, 38%, and 60%, respectively. With ischemia duration of 25 minutes hearts generated 57% of developed pressure compared to control hearts; however, with 30 minutes of ischemia, the heart function decreased to less than 50% of control hearts and deteriorated further.…”

The Commonalities and Differences in Mitochondrial Dysfunction Between ex vivo and in vivo Myocardial Global Ischemia Rat Heart Models: Implications for Donation After Circulatory Death Research

“…Based on these observations, we selected 25 min of ischemia time as the maximal duration of ischemia from which the heart has the potential to recover function. In a study by Wyss et al (2019), a correlation was sought between the varying warm ischemia times (21, 24, 27, 30, and 33 min) and myocardial/mitochondrial function in an ex vivo ischemia rat heart model. They noticed that ex vivo ischemia time of 27 min or more correlated with a significant decline in myocardial and mitochondrial function, including an increase in cytochrome c release (Wyss et al, 2019).…”

Mechanical Postconditioning Promotes Glucose Metabolism and AMPK Activity in Parallel with Improved Post-Ischemic Recovery in an Isolated Rat Heart Model of Donation after Circulatory Death

“…An isolated heart model of DCD heart transplantation was used, as previously reported [26,45,47,53,54], with male Wistar rats (Janvier Labs, Le Genest-Saint-Isle, France) of 10 to 11 weeks of age to ensure mature cardiac metabolism [55] and to represent young, adult human DCD donors. Rats were housed under standard conditions at a controlled room temperature with a 12-h light-dark cycle and access to water and food ad libitum.…”

“…To obtain data for cardiac function, a micro-tip pressure catheter (Millar, Houston, TX, USA) placed in the left ventricle was used to continuously measure heart rate (HR), peak systolic pressure, developed pressure (DP), minimum and maximum first derivatives of left ventricular pressure (dP/dt min and dP/dt max). Left ventricular work (LV work) was calculated as previously described [45,47,53,54]: DP × HR.…”