Mitochondrial movement and morphology depend on an intact actin cytoskeleton inAspergillus nidulans

Suelmann, Rüdiger; Fischer, Reinhard

doi:10.1002/(sici)1097-0169(200001)45:1<42::aid-cm4>3.0.co;2-c

Cited by 83 publications

(8 citation statements)

References 51 publications

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“…Wild type (strain BN38.9) and Δatg17 mutant (strain AW20.10) showed vacuolar localization of mitochondrially targeted GFP upon carbon starvation, whereas both Δatg1 and Δatg8 mutants (strains BN39.2 and BN40.8, respectively) showed cytosolic localization. Under nutrient-rich conditions, all strains showed fluorescent signals resembling tubular mitochondrial networks as described by Suelmann and Fischer (2000), as exemplarily shown for the Δatg1 and Δatg8 strains in Fig. 7.…”

Section: Scale Bar 5 μMsupporting

confidence: 66%

“…2) were cultured in carbon-limited bioreactor batch cultures and monitored using fluorescent microscopy. Hyphae from the exponential growth phase showed fluorescent tubular structures resembling those described by Suelmann and Fischer (2000), and no difference in mitochondrial morphology was observed between the three strains (see Fig. 7a).…”

Section: Scale Bar 5 μMmentioning

confidence: 60%

“…To monitor autophagy-mediated turnover of the mitochondria, also referred to as mitophagy (Kanki et al 2011), induced by carbon starvation, wild type and atg deletion strains with constitutive expression of mitochondrially targeted GFP (see Table 1) were generated. The approach described by Suelmann and Fischer (2000), who showed that N-terminal fusion of the mitochondrial targeting sequence from the citrate synthase A to a fluorescent protein efficiently labeled mitochondria in A. nidulans, was followed. Similar to A. nidulans, wild type (strain BN38.9) and atg mutant reporter strains (strains BN39.2, BN40.8, and AW20.10) showed fluorescent tubular structures inside hyphae under nutrient-rich conditions (as examplarily shown for Δatg1 and Δatg8 in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The vector for the constitutive expression of mitochondrially targeted green fluorescent protein (GFP) was constructed as follows. A 1.1-kb NcitA::gfp fragment was PCR-amplified from genomic DNA of the Aspergillus nidulans strain SRS29 (Suelmann and Fischer 2000), blunt-end ligated into pJET1.2 (Fermentas, St. Leon-Rot, Germany), and sequenced. Subsequently, the fragment was excised using BglII and BamHI restriction enzymes and ligated into the 3.5-kb BglII-BamHI backbone of pAN52-1N (GenBank: Z32697.1).…”

Section: Construction Of Strainsmentioning

confidence: 99%

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Autophagy promotes survival in aging submerged cultures of the filamentous fungus Aspergillus niger

Nitsche

Welzen

Lamers

et al. 2013

Appl Microbiol Biotechnol

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Autophagy is a well-conserved catabolic process constitutively active in eukaryotes that is involved in maintaining cellular homeostasis by the targeting of cytoplasmic content and organelles to vacuoles. Autophagy is strongly induced by the limitation of nutrients including carbon, nitrogen, and oxygen and is clearly associated with cell death. It has been demonstrated that the accumulation of empty hyphal compartments and cryptic growth in carbon-starved submerged cultures of the filamentous fungus Aspergillus niger is accompanied by a joint transcriptional induction of autophagy genes. This study examines the role of autophagy by deleting the atg1, atg8, and atg17 orthologs in A. niger and phenotypically analyzing the deletion mutants in surface and submerged cultures. The results indicate that atg1 and atg8 are essential for efficient autophagy, whereas deletion of atg17 has little to no effect on autophagy in A. niger. Depending on the kind of oxidative stress confronted with, autophagy deficiency renders A. niger either more resistant (menadione) or more sensitive (H2O2) to oxidative stress. Fluorescence microscopy showed that mitochondrial turnover upon carbon depletion in submerged cultures is severely blocked in autophagy-impaired A. niger mutants. Furthermore, automated image analysis demonstrated that autophagy promotes survival in maintained carbon-starved cultures of A. niger. Taken together, the results suggest that besides its function in nutrient recycling, autophagy plays important roles in physiological adaptation by organelle turnover and protection against cell death upon carbon depletion in submerged cultures.

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Section: Scale Bar 5 μMsupporting

confidence: 66%

Section: Scale Bar 5 μMmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 99%

Section: Construction Of Strainsmentioning

confidence: 99%

See 2 more Smart Citations

Autophagy promotes survival in aging submerged cultures of the filamentous fungus Aspergillus niger

Nitsche

Welzen

Lamers

et al. 2013

Appl Microbiol Biotechnol

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“…The nucleotide sequence corresponding to the first 76 amino acids of the L. maculans citrate synthase gene ( Lema_T101280.1 ) was amplified using primers AU268 and AU269 (Supplementary Table S1) off the genomic DNA of strain M1. Suelmann and Fischer (2000) showed that the corresponding sequence from A. nidulans was sufficient to direct GFP localization to the mitochondria. The coding region of the GFP gene was amplified using primers AU108 and AU68 off plasmid PLAU17 (Idnurm et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces variotii (Eurotiales)

et al. 2018

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Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared to have been mutated by repeat-induced point (RIP) mutations. We developed methods for genetic transformation of P. variotii, including forward and reverse genetics as well as crossing techniques. Using transformation and crossing, RIP activity was identified, demonstrating for the first time that RIP is an active process within the order Eurotiales. A consequence of RIP is likely reflected by a reduction in numbers of genes within gene families, such as in cell wall degradation, and reflected by growth limitations on P. variotii on diverse carbon sources. Furthermore, using these transformation tools we characterized a conserved protein containing a domain of unknown function (DUF1212) and discovered it is involved in pigmentation.

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Structural Organization and Dynamics of Mitochondria in the Cells in Vivo

Kuznetsov

2007

Molecular System Bioenergetics

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Mitochondrial movement and morphology depend on an intact actin cytoskeleton inAspergillus nidulans

Cited by 83 publications

References 51 publications

Autophagy promotes survival in aging submerged cultures of the filamentous fungus Aspergillus niger

Autophagy promotes survival in aging submerged cultures of the filamentous fungus Aspergillus niger

Genomic and Genetic Insights Into a Cosmopolitan Fungus, Paecilomyces variotii (Eurotiales)

Structural Organization and Dynamics of Mitochondria in the Cells in Vivo

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