Mitochondrial OPA1 cleavage is reversibly activated by differentiation of H9c2 cardiomyoblasts

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

“…Meanwhile, the S-OPA1 significantly increased in model group and honokiol treatment reversed this change. Taken L-OPA1/S-OPA1 ratio as an index 89 , we found the ratio was significantly decreased in the gastrocnemius muscle of the SOD1-G93A mice while honokiol eliminated the reduction. No significant alterations were found in PINK1 protein levels SOD1-G93A mice, indicating mitophagy shows slight effect on honokiol-regulated mitochondrial function.…”

“…Long OPA1 isoforms (L-OPA1) carry out inner membrane fusion when MMP is intact. However, L-OPA1 isoforms are cleaved to short (S-OPA1) isoforms upon loss of MMP, collapsing the mitochondrial network and promoting apoptosis 89 , 90 . In the SOD1-G93A cells and the spinal cord of the SOD1-G93A mice, the L-OPA1 was decreased obviously in ALS model group and honokiol clearly reversed the changes, while the S-OPA1 was unchanged in each group.…”

The Role of Mitochondria in Metabolic Syndrome–Associated Cardiomyopathy

“…These findings are consistent with those reported by Quirós et al; they found that OPA1 disturbance causes insulin resistance, impaired glucose homeostasis, and disordered thermogenesis in Oma1 knockout mice [ 188 ]. Oma1 –/– mice developed metabolic disorders after HFD, highlighting the role of OPA1 in regulating mitochondrial function [ 189 ]. And Parra et al also reported that the impact of insulin on mitochondrial metabolism was impaired in OPA1 −/− and Mfn2 −/− cells [ 187 ].…”