Mitogenic activity from trout embryos.

Collodi, Paul; Barnes, David W.

doi:10.1073/pnas.87.9.3498

Cited by 59 publications

(28 citation statements)

References 26 publications

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“…Trout embryo extract promotes the serum-free growth of several established piscine cell lines, and its effect on the cells cannot be mimicked by purified mammalian growth factors (Collodi and Barnes, 1990). The partially purified mitogenic activity is heat stable and associates with two fractions of 25 and 60 kilo-daltons when analyzed by gel filtration chromatography or SDS polyacrylamide gel electrophoresis.…”

Section: Exposure Of Zemh Cells To Tcddmentioning

confidence: 99%

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Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues

et al. 1992

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The zebrafish is a popular model for studies of vertebrate development and toxicology. However, in vitro approaches with this organism have not been fully exploited because cell culture systems have been unavailable. We developed methods for the culture of cells from blastula-stage diploid and haploid zebrafish embryos, as well as cells from the caudal and pelvic fin, gill, liver, and viscera of adult fish. The haploid embryo-derived cells differentiated in culture to a pigmented phenotype and expressed, upon exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin, a protein that was immunologically and functionally similar to rainbow trout cytochrome P450IA1. Zebrafish cultures were grown in a complex basal nutrient medium supplemented with insulin, trout embryo extract, and low concentrations of trout and fetal bovine serum; they could not be maintained in conventional culture medium containing a high concentration of mammalian serum. Using calcium phosphate-mediated transfection, a plasmid constructed for use in mammalian cells was introduced into zebrafish embryo cell cultures and expressed in a stable manner. These results indicated that the transfection procedures utilized in mammalian systems can also be applied to zebrafish cell cultures, providing a means for in vitro alteration of the genotype and phenotype of the cells.

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Section: Exposure Of Zemh Cells To Tcddmentioning

confidence: 99%

“…Previously, we reported mitogenic activity in trout embryo extract that promotes growth of established fish cell lines and primary salmonid embryo cell cultures in medium containing little or no serum (Collodi and Barnes, 1990). In this paper we describe application of this approach to the culture of zebrafish cells.…”

Section: Introductionmentioning

confidence: 99%

Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues

et al. 1992

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“…For the culture of established cell lines, culture medium was supplemented with the following four components: fish serum, FBS, bFGF, and embryo extracts, which promote proliferation (Collodi and Barnes, 1990;Boilly et al, 2000;Freshney, 2010). Knowledge of the dependence of the cell lines on these factors makes it possible to evaluate cellular properties and optimize culture conditions.…”

Section: A C D Bmentioning

confidence: 99%

Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

Lee¹,

Kim²,

Nam³

et al. 2013

Fisheries and aquatic sciences

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The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established from 41 total trials (4.9%). The two cell lines displayed typical in vitro morphology and have been cultured for >121 passages, which corresponds to 293 days. The doubling times of the cell lines were 29.84 and 28.59 h, respectively, and both possessed the potential to expand in a clonal manner, albeit with significant differences between the two cell lines. The absence of any of the four main medium supplements; i.e., fish serum, fetal bovine serum, basic fibroblast growth factor, and medaka embryo extract, significantly inhibited growth. The proportion of cells possessing normal chromosome number was 45% and 46.7% of the cell lines, respectively. Taken together, two cell lines that proliferate continuously were established from marine medaka and these cell lines may provide a basic tool for characterizing the unique features of this fish species.

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“…, , (Catla catla; Dash et al, 2010) (Oryzias dancena; Lee et al, 2013) 7 , (Lateolabrax japonicas; Chen et al, 2003a) (Chrysophrys major; Chen et al, 2003b) 36 , (Scophtalmus maximus, Holen and Hamre, 2003) 3 . , , (Collodi and Barnes, 1990;Na et al, 2009), (Assouline et al, 2002;Maures et al, 2002;Schimmang, 2007;Hanington et al, 2008), .…”

Section: 서 론 [Embryonic Stem Cells (Escs)]mentioning

confidence: 99%

“…(Oncorhynchus mykiss) 21 28 (Oncorhynchus tshawytscha) , 21 (Collodi and barnes, 1990), , (Cucina et al, 2006;Na et al, 2009). …”

Section: 서 론 [Embryonic Stem Cells (Escs)]mentioning

confidence: 99%

Effects of the Developmental Stage of Extract Donor Embryos on the Culture of Marine Medaka Oryzias dancena Embryonic Stem Cell-like Cells

Ryu¹,

Gong²

2017

Korean Journal of Fisheries and Aquatic Sciences

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Optimizing the conditions for stem cell culture is an essential prerequisite for the efficient utilization of stem cells. In the culture of fish embryonic stem cells (ESCs) or ESC-like cells, embryo extracts are important for stable growth, but there is no rule for determining the developmental stage of the embryos used to obtain extracts. Therefore, this study investigated the effects of the developmental stage of extract donor embryos on the culture of Oryzias dancena ESC-like cells. O. dancena ESC-like cells were cultured in different media containing each of four types of embryo extract depending on the developmental stage of the extract donor embryos. Growth, morphology, colony-forming ability, alkaline phosphatase (AP) activity, and embryoid body (EB) formation of the cells were investigated. While the developmental stage of the extract donor embryos did not influence the growth, morphology, AP activity, or EB formation of ESC-like cells, colony-forming ability was affected and the pattern of the effects differed completely between the two ESC-like cells investigated. These results suggest that the developmental stage of extract donor embryos should be selected carefully for the culture of ESC-like cells, according to the research purpose and type of cell line.

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Mitogenic activity from trout embryos.

Cited by 59 publications

References 26 publications

Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues

Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues

Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

Effects of the Developmental Stage of Extract Donor Embryos on the Culture of Marine Medaka Oryzias dancena Embryonic Stem Cell-like Cells

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