Mitosis-related gene CENP-U as a potential biomarker in malignancy

Shao, Changjian; Wang, Yuanyong; Duan, Hongtao; Ding, Peng; Zhang, Yimeng; Ning, Jiayi; Han, Jing; Jiang, Tao; Yan, Xiaolong

doi:10.21037/atm-21-6516

Cited by 5 publications

(5 citation statements)

References 46 publications

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“…These results suggest that CENPO plays an important immunomodulatory role and may serve as a potential immunotherapeutic relevant biomarker. Previous studies reported that the CENP family is associated with immune function [6,43], and our bioinformatics results also demonstrated that CENPO is associated with immune regulation. We then demonstrated by in vitro experiments that CENPO was highly expressed in A549 and H1299 cell lines and that the knockdown of CENPO significantly inhibited the proliferative activity, migration, and invasive ability of A549 and H1299 cells, suggesting that CENPO may affect LUAD metastasis.…”

Section: Discussionsupporting

confidence: 81%

Prognostic and immune infiltrative biomarkers of CENPO in pan–cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Wang

Liu

et al. 2023

BMC Cancer

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Background The centromere protein O (CENPO) is an important member of the centromere protein family. However, the role of CENPO in pan–cancer and immune infiltration has not been reported. Here, we investigated the role of CENPO in pan–cancer and further validated its role in lung adenocarcinoma (LUAD) by in vitro experiments. Method The UCSC Xena database and The Cancer Genome Atlas (TCGA)–LUAD data were used to assess the expression levels of CENPO. The potential value of CENPO as a diagnostic and prognostic biomarker for pan–cancer was evaluated using TCGA data and the GEPIA database. The -expression profiles of LUAD patients and the corresponding clinical data were downloaded for correlation analysis. The role of CENPO in immune infiltration was investigated using the UCSC Xena database. Subsequently, qRT–PCR was performed to detect the expression of CENPO. Cell proliferation, migration, and invasion were determined using CCK–8, wound–healing assay, and transwell assay, respectively. Results CENPO is highly expressed in most cancers, and the upregulation of CENPO is associated with poor prognosis in many cancers. CENPO expression correlates with age, TNM stage, N stage, T stage, and receipt of radiotherapy in LUAD patients, and LUAD patients with high CENPO expression have poorer overall survival (OS) and disease–free survival (DFS). In addition, CENPO expression is associated with immune cell infiltration and immune checkpoint inhibitors. Moreover, the expression of CENPO was closely related to the expression of tumor mutational load and microsatellite instability. In vitro experiments showed that CENPO expression was increased in LUAD cell lines and that knockdown of CENPO significantly inhibited the proliferation, cell invasion, and migration ability of LUAD cells. Conclusion CENPO may be a potential pan–cancer biomarker and oncogene, especially in LUAD. In addition, CENPO is associated with immune cell infiltration and may serve as a new molecular therapeutic target and effective prognostic marker for LUAD.

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Section: Discussionsupporting

confidence: 81%

Prognostic and immune infiltrative biomarkers of CENPO in pan–cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Wang

Liu

et al. 2023

BMC Cancer

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“…These results suggest that CENPO plays an important immunomodulatory role and may serve as a potential immunotherapeutic relevant biomarker. Previous studies reported that the CENP family is associated with immune function [6,40], and our bioinformatics results also demonstrated that CENPO is associated with immune regulation. We then demonstrated by in vitro experiments that CENPO was highly expressed in A549 and H1299 cell lines and that the knockdown of CENPO signi cantly inhibited the proliferative activity, migration, and invasive ability of A549 and H1299 cells, suggesting that CENPO may affect LUAD metastasis.…”

Section: Discussionsupporting

confidence: 81%

Prognostic and immune infiltrative biomarkers of CENPO in pan- cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Wang¹,

Liu³

et al. 2023

Preprint

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Background The centromere protein O (CENPO) is an important member of the centromere protein family. However, the role of CENPO in pan–cancer and immune infiltration has not been reported. Here, we investigated the role of CENPO in pan–cancer and further validated its role in lung adenocarcinoma (LUAD) by in vitro experiments. Method The UCSC Xena database and The Cancer Genome Atlas (TCGA)–LUAD data were used to assess the mRNA expression levels of CENPO. The potential value of CENPO as a diagnostic and prognostic biomarker for pan–cancer was evaluated using TCGA data and the GEPIA database. The mRNA expression profiles of LUAD patients and the corresponding clinical data were downloaded for correlation analysis. The role of CENPO in immune infiltration was investigated using the UCSC Xena database. Subsequently, RT–QPCR was performed to detect the expression of CENPO. Cell proliferation, migration, and invasion were determined using CCK–8, wound–healing assay, and transwell assay, respectively. Results CENPO is highly expressed in most cancers, and the upregulation of CENPO is associated with poor prognosis in many cancers. CENPO expression correlates with age, TNM stage, N stage, T stage, and receipt of radiotherapy in LUAD patients, and LUAD patients with high CENPO expression have poorer overall survival (OS) and disease–free survival (DFS). In addition, CENPO expression is associated with immune cell infiltration and immune checkpoint inhibitors. Moreover, the expression of CENPO was closely related to the expression of tumor mutational load and microsatellite instability. In vitro experiments showed that CENPO expression was increased in LUAD cell lines and that knockdown of CENPO significantly inhibited the proliferation, cell invasion, and migration ability of LUAD cells. Conclusion CENPO may be a potential pan–cancer biomarker and oncogene, especially in LUAD. In addition, CENPO is associated with immune cell infiltration and may serve as a new molecular therapeutic target and effective prognostic marker for LUAD.

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“…CENPs are important members of the centromere and kinetochore, which determine the separation of chromosomes during mitosis and meiosis. Many CENPs have been currently identified, including CENPA, CENPH, CENPI, and CENPU (7)(8)(9)(10)(11). Among them, CENPA plays a significant role in cancer.…”

Section: Introductionmentioning

confidence: 99%

Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker

et al. 2022

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BackgroundCentromere protein L (CENPL) is an important member of the centromere protein (CENP) family. However, the correlation between CENPL expression and cancer development and immune infiltration has rarely been studied. Here, we studied the role of CENPL in pan-cancer and further verified the results in lung adenocarcinoma (LUAD) through in vitro experiments.MethodsThe CENPL expression level was studied with TIMER 2.0 and Oncomine databases. The potential value of CENPL as a diagnostic and prognostic biomarker in pan-cancer was evaluated with the TCGA database and GEPIA. The CENPL mutation character was analyzed using the cBioPortal database. The LinkedOmics and CancerSEA databases were used to carry out the function analysis of CENPL. The role of CENPL in immune infiltration was studied using the TIMER and TISIDB websites. Moreover, the expression of CENPL was detected through RT-qPCR and Western blotting. Immunohistochemistry was used to evaluate the infiltration level of CD8+ T cells. Cell proliferation was detected by EdU and CCK8. A flow cytometer was used to analyze the influence of CENPL in cell cycle and apoptosis.ResultsCENPL was increased in most of the cancers. The upregulation and mutation of CENPL were associated with a poorer prognosis in many cancers. The results showed a significant positive correlation between CENPL and myeloid-derived suppressor cell (MDSC) infiltration and a negative correlation between CENPL and T-cell NK infiltration in most of the cancers. CENPL regulated cell proliferation and cell cycle, and was negatively correlated with the inflammation level of LUAD. The in vitro experiments suggested that CENPL was increased in LUAD tissue and cell lines. There was a negative correlation between CENPL expression and CD8+ T-cell infiltration. The knockdown of CENPL significantly suppressed the expression of CDK2 and CCNE2, and induced G0/G1 arrest and apoptosis of LUAD.ConclusionsCENPL may function as a potential biomarker and oncogene in pan-cancer, especially LUAD. Furthermore, CENPL was associated with immune cell infiltration in pan-cancer, providing a potential immune therapy target for tumor treatment.

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Mitosis-related gene CENP-U as a potential biomarker in malignancy

Cited by 5 publications

References 46 publications

Prognostic and immune infiltrative biomarkers of CENPO in pan–cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Prognostic and immune infiltrative biomarkers of CENPO in pan–cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Prognostic and immune infiltrative biomarkers of CENPO in pan- cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Pan-Cancer and Single-Cell Analysis Reveals CENPL as a Cancer Prognosis and Immune Infiltration-Related Biomarker

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