Mitral and Tufted Cells Are Potential Cellular Targets of Nitration in the Olfactory Bulb of Aged Mice

Yang, Myung Jae; Sim, Sooyeon; Jeon, Ji Hyun; Jeong, Eojin; Kim, Hyoung-Chin; Park, Yong‐Jin; Kim, In‐Beom

doi:10.1371/journal.pone.0059673

Cited by 14 publications

(15 citation statements)

References 74 publications

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“…All animals were maintained at Korea Research Institute of Bioscience and Biotechnology (Ochang, Korea). According to Yang et al (2013), mice aged 2-, 6-, 18-or 30-month old were classified as young, adult, middle-aged, and aged animals, respectively. The mice were housed in temperature (22 ± 2°C)-and humidity (50 ± 5 %)-controlled rooms with a 12-h light/dark cycle for at least 1 week before experimentation.…”

Section: Animalsmentioning

confidence: 99%

Effects of age increase on hepatic expression and activity of cytochrome P450 in male C57BL/6 mice

Kwak

Kim

et al. 2014

Arch. Pharm. Res.

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Effects of aging on hepatic expression and activity of cytochrome P450 (CYP) isoforms were investigated in male mice aged 2, 6, 18, and 30 months. Microsomal protein, total CYP, cytochrome b5 and NADPH-dependent cytochrome P450 reductase contents in liver were fully expressed in young (2-month-old) mice. Neither Cyp1a1 nor Cyp2c was detected in any aged mice. And Cyp1a2 was maximally expressed at 2 months and decreased with age. Hepatic levels of Cyp2b10 and Cyp3a11 were decreased in 30-month-old mice. Hepatic Cyp2e1 levels were constantly maintained from 2-month to 30-month old mice. Hepatic activities of ethoxyresorufin-O-deethylase and methoxyresorufin-O-demethylase were gradually decreased after 6 months. The 30-month-old mice exhibited the lowest activity of midazolam 1'-hydroxylase. Pentoxyresorufin-O-depenthylase activity was decreased in 30-month-old mice, but not statistically significant. There were no significant differences in hepatic activities of chlorzoxazone 6-hydroxylase and midazolam 4-hydroxylase. The present study shows that increasing age, especially 30-month-old mice, leads to decrease in expression and activity of hepatic CYP isoforms, suggesting that aging mice exhibit poor hepatic drug-metabolizing capacity.

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Section: Animalsmentioning

confidence: 99%

Effects of age increase on hepatic expression and activity of cytochrome P450 in male C57BL/6 mice

Kwak

Kim

et al. 2014

Arch. Pharm. Res.

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“…Murdaugh et al [23] reported that 3-nitrotyrosine (a neurochemical marker of peroxynitrite) and nitrated A2E levels increase in human Bruch's membrane with age. Yang et al [24] examined the age-related changes in 3-nitrotyrosine in the olfactory bulb of mice. They found increased 3-nitrotyrosine expression in mitral and tufted cells in the olfactory bulb of aged mice.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Age-Related Macular Degeneration and Olfactory Function

et al. 2015

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Background: Olfactory dysfunction is a common symptom of many neurodegenerative diseases, and age-related macular degeneration (AMD) is a late-onset neurodegenerative disease. Objective: Thus, the aim of this study was to investigate olfactory functions in patients with AMD. Methods: A total of 69 subjects with AMD and 69 age- and sex-matched healthy controls were enrolled. After a complete ophthalmic evaluation, the AMD patients were subclassified as early- and late-stage AMD. Psychophysical testing of olfactory function was performed using the validated Sniffin' Sticks test. Results: This study was carried out in 138 subjects, with a mean age of 74.3 ± 8.9 years (range 51-89). The current investigation showed the following two major findings: (1) patients with AMD had decreased olfactory abilities, especially in odor discrimination and odor identification, even at early stages compared to controls, whereas patients had decreased olfactory abilities in all subtasks of olfactory testings in advanced stages of AMD disease, and (2) as the visual acuity of AMD patients decreased, the olfactory abilities of these patients worsened. Conclusion: This study demonstrated that AMD had significant negative effects on all orthonasal olfactory tasks, particularly in advanced stages. Similar to other neurodegenerative diseases, odor discrimination and identification seemed to be more affected than odor detection threshold tasks.

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“…While some authors report “middle-age” as early as 8 month old [51], [52], others have considered 10–12 month as the appropriate range [53], [54]. Yet other investigators have gone beyond these age ranges and used 17–18 month mice to refer to “middle-age” [55], [56]. In our study, we used 6–8 month old mice to characterize the inflammatory response associated with the upper age range for the mature adult group, which is typically approximately 6 months [ 57 ] .…”

Section: Discussionmentioning

confidence: 99%

Combined In Silico, In Vivo, and In Vitro Studies Shed Insights into the Acute Inflammatory Response in Middle-Aged Mice

et al. 2013

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We combined in silico, in vivo, and in vitro studies to gain insights into age-dependent changes in acute inflammation in response to bacterial endotoxin (LPS). Time-course cytokine, chemokine, and NO2 −/NO3 − data from “middle-aged” (6–8 months old) C57BL/6 mice were used to re-parameterize a mechanistic mathematical model of acute inflammation originally calibrated for “young” (2–3 months old) mice. These studies suggested that macrophages from middle-aged mice are more susceptible to cell death, as well as producing higher levels of pro-inflammatory cytokines, vs. macrophages from young mice. In support of the in silico-derived hypotheses, resident peritoneal cells from endotoxemic middle-aged mice exhibited reduced viability and produced elevated levels of TNF-α, IL-6, IL-10, and KC/CXCL1 as compared to cells from young mice. Our studies demonstrate the utility of a combined in silico, in vivo, and in vitro approach to the study of acute inflammation in shock states, and suggest hypotheses with regard to the changes in the cytokine milieu that accompany aging.

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Mitral and Tufted Cells Are Potential Cellular Targets of Nitration in the Olfactory Bulb of Aged Mice

Cited by 14 publications

References 74 publications

Effects of age increase on hepatic expression and activity of cytochrome P450 in male C57BL/6 mice

Effects of age increase on hepatic expression and activity of cytochrome P450 in male C57BL/6 mice

The Relationship between Age-Related Macular Degeneration and Olfactory Function

Combined In Silico, In Vivo, and In Vitro Studies Shed Insights into the Acute Inflammatory Response in Middle-Aged Mice

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