Mixed-function oxidase involvement in the biochemistry of insecticide synergists

Casida, John E.

doi:10.1021/jf60171a013

Cited by 360 publications

(157 citation statements)

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“…Mixed function oxidase, glutathione-s-transferase and esterase are involved in many insects in insecticide resistance mechanisms (Narahashi et al, 1995) due to their ability to detoxify insecticides and other xenobiotics (Li et al, 2007). Many synergists such as piperonyl butoxide (PBO), diethyl maleate (DEM) and S,S,S-tributyl phosphoro trithioate (DEF) used at non-toxic doses are known to inhibit monoxygenase, glutathione-s-transferase and esterase activities, respectively (Casida, 1970;Scott, 1990). However, work on insecticide resistance and mechanisms of resistance in chrysopid predators is very scanty and this is first kind of such study in India.…”

Section: Introductionmentioning

confidence: 99%

Detection of insecticide resistance and mechanisms of resistance in field populations of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) collected from different geographical locations in India

Venkatesan

Mahiba²,

Jalali

et al. 2018

Journal of Biological Control

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ABSTRACT:The toxic effect of commonly used insecticides in cotton fields was studied on 9 populations of Chrysoperla zastrowi sillemi (Esben-Petersen), an important predator of sucking pests collected in India. The dose mortality bioassay against 3-days old larvae was determined using three insecticides viz., endosulfan, fenvalerate and acephate by topical bioassay method. Mechanism of resistance to the above mentioned insecticides were determined without and with three metabolic inhibitors (synergists), viz., piperonyl butoxide (PBO), S,S,S-tributyl-phosphorotrithioate (DEF) and diethyl maleate (DEM). Among the populations, resistant ratios (RR) of CZS-8 was significantly higher i.e. 50.36., 66.11 and 277.51-fold for endosulfan, fenvalerate and acephate, respectively compared to susceptible population (CZS-10). The CZS-8 was selected for synergism study it showed higher LC 50 values and resistance ratio for all three insecticides. It showed 8.97-fold, 18.49-fold and 6.38-fold increase in synergism ratio for endosulfan indicating the resistance was strongly synergised by PBO, DEF and DEM. Similarly for fenvalerate, CZS-8 showed 8.69-fold and 3.63-fold significant increase in synergism ratio by DEF and DEM, respectively and for acephate, CZS-8 showed 54.82-fold, 150.87-fold and 113.52-fold significant increase in synergism ratio indicating that the resistance could be due to cytochrome p-450, esterase and glutathione s-transferase activity. The study indicated that the field population of C. z. sillemi developed resistance to different groups of insecticides. Among different geographical populations, CZS-8 collected from Sriganganagar, was recorded as most resistant.

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Section: Introductionmentioning

confidence: 99%

Detection of insecticide resistance and mechanisms of resistance in field populations of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) collected from different geographical locations in India

Venkatesan

Mahiba²,

Jalali

et al. 2018

Journal of Biological Control

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“…Such information is a prerequisite to develop strategies to maximize its efficacy in the field. It is well established that piperonyl butoxide (PBO) is an effective synergist for synthetic pyrethroids due to its ability to inhibit the monooxygenases as detoxifying enzymes (Casida, 1970;Jao, 1974). Recently, Gunning et al (1998) have reported that in the cotton aphid, Aphis gossypii (Hemiptera: Aphididae), piperonyl butoxide (PBO) acted as an inhibitor of esterases, as it is a well known inhibitor of microsomal monooxygenase, which are involved in the metabolism and detoxification of virtually all insecticides (Feyereisen, 1999;Hemingway and Ranson, 2000).…”

Section: Introductionmentioning

confidence: 99%

Synergist efficacy of piperonyl butoxide with deltamethrin as pyrethroid insecticide on Culex tritaeniorhynchus (Diptera: Culicidae) and other mosquitoe species

Fakoorziba

Eghbal

Vijayan

2008

Environmental Toxicology

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Continuous and indiscriminate use of pesticides, especially in tropical countries for public health or agriculture purpose, has led many vector populations to become resistant to organochlorides, organophosphates, and even to carbamates and pyrethroids. Development of resistance by a vector population has been one of the reasons for the failure of the control measures in many countries. This investigation demonstrates the efficacy of piperonyl-butoxide (PBO) with deltamethrin, as pyrethroid insecticide, against the field-collected mosquitoe larvae of five species, Aedes aegypti, Anopheles culicifacies, An. stephensi, An. vagus, and Culex quinqufasciatus, and two morphological variants of Cx. tritaeniorhynchus (type A from grand pools of Mysore city and type B from rice fields of Mandya district). For testing the synergistic effect of PBO, stock solutions of deltamethrin and PBO were mixed in 1:6 ratio. The synergistic ratio and the percent suppression in deltamethrin tolerance were calculated by using LC 50 values. From the results, it is clear that, PBO is an effective synergist with deltamethrin against all of species undertaken in this investigation. So, it is suggested that PBO is a good synergist in this area for decreasing the use of pesticides in environment in vector control. #

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“…Pode ainda reagir com sítios do sistema enzimático, inibindo a excreção do inseticida (CASIDA, 1970).…”

Section: Introductionunclassified

Sinérgico alternativo para inseticidas inibidores de acetilcolinesterase

Fazolin

Estrela

Medeiros

et al. 2017

AGRO@MBIENTE ON-LINE

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A importância da utilização de sinergistas está relacionada à minimização da quantidade de inseticida químico necessária para o controle de insetos, podendo contribuir com a diminuição da contaminação ambiental e preservação de insetos benéficos. Assim, objetivou-se com este trabalho avaliar o efeito sinérgico e da homogeneidade de resposta de lagartas de Spodoptera frugiperda (J. E. Smith, 1797) às doses subletais do óleo essencial de Piper aduncum L. (OEPA) em combinações com formulações de organofosforados e carbamato, comparadas àquelas com butóxido de piperonila (PBO). Foram obtidos fatores de sinergismo (FS) para comparação dos tratamentos entre si. Por contato residual, evidenciou-se significativa potencialização dos inseticidas Profenofós (FS= 5,4 – 7,7), Fenitrotiona (FS= 3,9 – 29,2) e Clorpirifós (FS= 3,4 – 7,8). Já por contato tópico ocorreu significativa potencialização dos inseticidas: Profenofós + ¼ da DL50 do OEPA (FS= 5,4), Fenitrotiona + ¼ da DL50 do OEPA (FS= 33,8) e Clorpirofós + 1/2 da DL50 do OEPA (FS= 2,5). O metomil não foi sinergizado pelo OEPA nas duas vias de contaminação e não apresentou aumento da pressão de seleção para resistência na população avaliada de lagartas de S. frugiperda. Não foi possível concluir sobre a homogeneidade de resposta e consequentemente da pressão de seleção com relação aos inseticidas organofosforados para S. frugiperda. Os resultados indicam que o óleo essencial de Piper aduncum apresenta potencial como sinergista para Fenitrotiona, Profenofós e Clorpirifós, podendo ser uma alternativa ao butóxido de piperonila.

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Mixed-function oxidase involvement in the biochemistry of insecticide synergists

Cited by 360 publications

References 134 publications

Detection of insecticide resistance and mechanisms of resistance in field populations of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) collected from different geographical locations in India

Detection of insecticide resistance and mechanisms of resistance in field populations of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) collected from different geographical locations in India

Synergist efficacy of piperonyl butoxide with deltamethrin as pyrethroid insecticide on Culex tritaeniorhynchus (Diptera: Culicidae) and other mosquitoe species

Sinérgico alternativo para inseticidas inibidores de acetilcolinesterase

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