Mixed Leucocyte Interaction involving Mouse Strains of Strong and Weak Allogeneic Specificities

Main, Raymond K.; Jones, Marjorie J.

doi:10.1038/2181251a0

Cited by 12 publications

(2 citation statements)

References 15 publications

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“…7744). A 50-ml sample of heparinized blood was layered onto a mixture of Hypaque (Winthrop) and methyl cellulose and the leukocyte-rich plasma thus obtained (Main and Jones, 1968) was shaken vigorously with silica gel (0.1 g/ml) for 90 min at 37" C; the suspension was then centrifuged at 50g for 3 min and the supernatant centrifuged on FicolljHypaque in the usual way. The monocyte-depleted (MD) subpopulation collected at the interface contained 0.05-0.3 % monocytes as indicated by non-specific esterase staining.…”

Section: Preparation and Fractionution Of Mononuclear Leukocytesmentioning

confidence: 99%

Long‐term T‐cell‐mediated immunity to Epstein‐Barr virus in man. II. Components necessary for regression in virus‐infected leukocyte cultures

Rickinson

Moss

Pope

1979

Intl Journal of Cancer

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Regression of EB-virus-induced transformation occurs exclusively in cultures of leukocytes from seropositive donors. Studies have shown that the strength of regression could be assayed in terms of the proportion of T cells which must be added to the autologous EB virus-infected T-cell-depleted population in order to establish regression in the culture. The in vitro regression phenomenon was strongly T-cell-dependent but did not require the presence of either monocytes or, as a potential antigenic stimulus, the residual viral envelope material on the surface of virus-infected B cells. The T-cell-depleted population from seropositive donors sometimes transformed (7/60 cultures) without the experimental addition of virus. Regression appeared to be independent of cytotoxic mechanisms involving anti-viral antibodies and not to be mediated by soluble factors released into culture medium.

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Section: Preparation and Fractionution Of Mononuclear Leukocytesmentioning

confidence: 99%

Long‐term T‐cell‐mediated immunity to Epstein‐Barr virus in man. II. Components necessary for regression in virus‐infected leukocyte cultures

Rickinson

Moss

Pope

1979

Intl Journal of Cancer

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“…Spleen and thymus cell suspensions were made by the same procedure as above. Peripheral blood lymphocytes were isolated from heparinized L LS7----r-rr -_ 7,-7-lX1o2 1~1 0~ iX1o4 iX1o5 1 x 1 0~ blood by the Hypaque-methocel method (Main & Jones 1968).…”

Section: Lymphocytesmentioning

confidence: 99%

The Mixed Kidney Cell – Lymphocyte Reaction in Rats

et al. 1976

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Optimal conditions for obtaining the proliferative response in mixed kidney cell‐lymphocyte culture (MKLC) have been determined in rats. The addition of 2‐mercaptoethanol to the culture medium was essential for successful stimulation of lymphocytes by allogeneic kidney cells. Proliferative responses by standard MLC and MKLC were compared in rats with the same or a different Ag‐B locus. In most strain combinations, there was a significant correlation between MLC and MKLC reactions. However, the DA‐ACI combination, compatible at the Ag‐B locus, consistently showed negative MLC and positive MKLC reactions. This suggested the possibility that this technique allowed the expression of kidney specific antigens.

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An In Vitro Model System for the Study of the Induction of Cellular Immunity

Simmons

Manson

1971

Advances in Experimental Medicine and Biology

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Mixed Leucocyte Interaction involving Mouse Strains of Strong and Weak Allogeneic Specificities

Cited by 12 publications

References 15 publications

Long‐term T‐cell‐mediated immunity to Epstein‐Barr virus in man. II. Components necessary for regression in virus‐infected leukocyte cultures

Long‐term T‐cell‐mediated immunity to Epstein‐Barr virus in man. II. Components necessary for regression in virus‐infected leukocyte cultures

The Mixed Kidney Cell – Lymphocyte Reaction in Rats

An In Vitro Model System for the Study of the Induction of Cellular Immunity

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