mmunohistochemistry, ultrastructure and pathology of gills of<i>Abramis brama</i>from Lake Mondsee, Austria, infected with<i>Ergasilus sieboldi</i>(Copepoda)

Dezfuli, Bahram Sayyaf; Giari, Luisa; Konecny, R.; Jaeger, Paul T.; Manera, Maurizio

doi:10.3354/dao053257

Cited by 81 publications

(82 citation statements)

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“…Martins et al (2001) analisaram brânquias de várias espécies como tambacu híbrido, pacu, tambaqui, piauçu, tilápia e curimbatá infectado por parasitas (dinoflagelados), e constataram aumento da produção de muco em todas as espécies. Resultado semelhante quanto ao número de células mucosas foi encontrado por Dezfuli et al (2003), utilizando a espécie Abramis brama infectada por ectoparasitas (Ergasilus sieboldi). Desta forma, a literatura mostra que as alterações na morfometria e no número de cé-lulas mucosas dependem do agente, cujo os peixes são expostos.…”

Section: Discussionunclassified

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

et al. 2009

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The behavior of the gill epithelium of tilapias cultured in tanks at different altitudes and interconnected with PVC pipes was analyzed. Gill filaments of four specimens from four tanks (T 1 , T 2 , T 3 e T 4 ) sequentially interconnected were submitted to histological routine to obtain 5-mm-thick cuts that were stained with HE or submitted to histochemistry reactions PAS + diastase solution or Alcian Blue pH 2.5 or Alcian Blue pH 1.0. Considering the intermediary, apical and basal regions of the filaments, the lamellar area was measured and the amount of mucous cells was counted. It was verified that oxygen, pH, and temperature decreased progressively as the water flew from one tank to another. Thus, an increase was realized of the amount of mucous cells and the lamellar area in T 2, as well as a progressive decrease of these measures on the tanks which received water from T 2 . Moreover, detachment of the gill epithelium, cellular hyperplasia in the interlamellar space, and telangectasias were observed in the fishes from T 2 , T 3 e T 4. It was concluded that the aquatic environment in tanks sequentially interconnected with PVC pipes suffers alterations from one tank to another, as that physico-chemical fluctuations reflect on the behavior of the gill epithelium through variations of the lamellar area and the amount of mucous cells.

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Section: Discussionunclassified

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

et al. 2009

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“…Schäperclaus (1954), Rogers (1969) and Alston and Lewis (1994) described the fusion of filaments by a solid mass of proliferated epithelial cells as well. Alston and Lewis (1994), who studied E. sieboldi infection of the tench, reported also haemorrhages and inflammation accompanied by granulocytosis, while Dezfuli et al (2003) found mucous cell proliferation and an increase in the number of eosinophilic granular cells and rodlet cells. These latter authors regarded rodlet cells as cells representing an inflammatory cell type.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and pathology of Sinergasilus lieni (Copepoda: Ergasilidae), a parasite of the silver carp and bighead, in Hungarian ponds

Molnár

Székely

2004

Acta Veterinaria Hungarica

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Sinergasilus lieni Yin, 1949, a well-known and pathogenic parasitic copepod in China and Russia, has been detected in Hungarian carp farms for the first time. The parasite infected the third-year generation of silver carp (Hypophthalmichthys molitrix) and bighead (Aristichthys nobilis). The gills of the infected fish specimens showed severe pathological changes. At the attachment sites of female copepods clubbing and fusing of the gill filaments were observed and in some parts of the pale or whitish hemibranchia deep indentations were recorded in places where the tips of the damaged filaments had broken off. Silver carp and bighead were infected at a similar rate, having 8 to 27 copepods attached to the end of the clubbed filaments or the proliferated epithelium of 2 to 10 fused filaments. In histological sections the head part of the parasite was found in a deep cavity of the proliferated epithelium, piercing its antennae deep into the tissues. Only the end of the filaments showed changes. In this part the proliferated epithelium was infiltrated by eosinophilic granular cells. In the central and basal parts of the hemibranchia the original structure of the filaments was preserved with intact secondary lamellae.

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“…Cut sections (7 µm thick) were stained with either haematoxylin-eosin, Azan-Mallory, periodic acid Schiff (PAS) and alcian blue/PAS stains or used for immunohistochemical analysis. Heart sections were processed using the indirect immunohistochemical method (peroxidase-anti-peroxidase immunocomplex) as outlined in Dezfuli et al (2002aDezfuli et al ( , 2003b. The antisera, the working dilution and the incubation time used for each of the neuromodulators are detailed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Histopathology, ultrastructure and immunohistochemistry of Coregonus lavaretus hearts naturally infected with Ichthyocotylurus erraticus (Trematoda)

Dezfuli¹,

Giari²,

Simoni³

et al. 2005

Dis. Aquat. Org.

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Histopathological, ultrastructural and immunohistochemical investigations were conducted on 26 specimens of powan Coregonus lavaretus (L.) from Loch Lomond (Scotland). The hearts of all 26 powan (15 females and 11 males) investigated harboured metacercariae of the digenean trematode Ichthyocotylurus erraticus (Rudolphi, 1809). The vast majority of metacercariae were located either singly or as an aggregation of white cysts on the surface of the bulbus arteriosus. The intensity of infection ranged from 2 to 200 larvae heart -1 , although the number of metacercariae found on male powan did not exceed 13. Histochemically, the parasite cyst wall gave a strong positive reaction with periodic acid schiff (PAS) and a faint positive signal with Azan-Mallory stain. All the metacercariae cysts were embedded in a granulomatous proliferation of heart epicardium tissue, forming a reactive fibroconnective capsule around the parasite. The capsule enclosing the parasite (produced by the host's reaction to the parasite) measured 13.57 to 90.20 µm (37.43 ± 3.56) in thickness. Within the capsule wall, eosinophilic granular cells (EGCs), granulocytes, melanocytes and, in some instances, partially degenerated or vacuolated epithelioid cells were observed in close proximity to the cyst wall. Pigment-bearing macrophages were scattered throughout the granulomatous host-tissue reaction and as macrophage aggregates (MAs) within the capsules surrounding parasites. Immunohistochemical tests were applied to infected heart sections using 12 different antisera. Nerve fibres immunoreactive to bombesin, substance P (SP), and atrial natriuretic peptide (ANP) antisera were observed in close proximity to the parasite larvae. The presence of a serotonin-like substance was also observed within host immune-cells surrounding trematode cysts. Large cells of the epicardium were found to be immunoreactive to met-enkephalin and vasoactive intestinal peptide (VIP) antisera but not immunoreactive to anti-protein gene-product 9.5 (PGP9.5) sera. KEY WORDS: Trematode infection · Immunohistochemistry · Neuromodulators · Heart · Coregonus lavaretusResale or republication not permitted without written consent of the publisher

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mmunohistochemistry, ultrastructure and pathology of gills ofAbramis bramafrom Lake Mondsee, Austria, infected withErgasilus sieboldi(Copepoda)

Cited by 81 publications

References 19 publications

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo

Occurrence and pathology of Sinergasilus lieni (Copepoda: Ergasilidae), a parasite of the silver carp and bighead, in Hungarian ponds

Histopathology, ultrastructure and immunohistochemistry of Coregonus lavaretus hearts naturally infected with Ichthyocotylurus erraticus (Trematoda)

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