MnSOD expression inhibited by electromagnetic pulse radiation in the rat testis

Abstract

Male Sprague Dawley rats were exposed to EMP irradiation of 100 kV/m peak-to-peak e-field intensity and different numbers of pulses. Rat sperm samples were prepared for analysis of sperm qualities; Testes were assessed by transmission electron microscopy and serum hormone concentrations were examined by radioimmunoassay; Enzymatic activities of Total-superoxide dismutase(T-SOD) and manganese-superoxide dismutase (MnSOD), the mRNA levels of MnSOD and cuprozinc-superoxide dismutase (CuZnSOD), and the density of malondialdehyde (MDA) were also determined. EMP irradiation did not affect spermatozoon morphology, micronucleus formation rate, sperm number or viability, but the acrosin reaction rate decreased at 24 h and 48 h and recovered by 72 h after irradiation as compared to the controls. The ultrastructure of rat testis displayed more serious damage at 24 h than at other time points (6 h, 12 h, 48 h). Serum levels of luteotrophic hormone (LH) and testosterone (T) were elevated in irradiated rats as compared to controls. After irradiation, enzymatic activities of T-SOD and MnSOD were reduced by 24 h, consistent with the changes observed in MnSOD mRNA expression; MDA content increased at 6 h in turn. These studies have quantified the morphological damage and dysfunction in the rat reproductive system induced by EMP. The mechanism of EMP induced damage may be associated with the inhibition of MnSOD expression.

1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells

“…Several studies have hypothesized that the imbalance between intracellular free radicals and antioxidant systems leading to oxidative stress may play an important role in the process of damage stemming from different types of exposure [ 12 , 13 , 14 , 29 , 30 , 31 ]. Especially in some experiments in vivo, the results indicated that anti-oxidative stress could induce by electromagnetic radiation [ 32 , 33 , 34 ]. Also, there is some negative effects which RF-EMR couldn’t induce oxidative stress [ 35 ].…”

Effects of extremely low-frequency electromagnetic fields (ELF-EMF) exposure on B6C3F1 mice

“…EMF inhibited MnSOD expression in the rat testis and induced oxidative stress on heart, lung, testis and liver tissues and testicular apoptosis [32][33][34]. Related animal study has also shown that exposure to ELF induced males mice spermatagonial cells apoptosis [35].…”

Pulsed microwave exposure real time and dynamically reduces reactive oxygen species in rat pheochromocytoma cells in vivo

“…Although RF‐EMF does not cause DNA strand breaks like ionizing radiation, it has been reported to result in damages of multiple targets such as in learning and memory, blood‐brain barrier, and reproductive systems . Most of the recorded effects of microwave are its oxidative stress, and central nervous system is believed to be vulnerable to microwave exposure . To date, several studies have shown that microwave causes various biological effects depending on its field strength, frequency, waveform, modulation, and duration of exposure.…”