Mobile genetic elements associated with carbapenemase genes in South American Enterobacterales

Reyes, Jorge E. Fernández; Melano, Roberto G.; Cárdenas, Paúl; Trueba, Gabriel

doi:10.1016/j.bjid.2020.03.002

Cited by 31 publications

(30 citation statements)

References 56 publications

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“…These issues could be related to the rapid dissemination of 3GC-R mediated by horizontal transfer in broiler farms (22). On the other hand, the (26). In Ecuador, bla KPC-2 is the most prevalent carbapenemase gene detected in E. coli in hospitals (27,28), and recently it was detected in an urban river in Citrobacter freundii (29).…”

Section: Discussionmentioning

confidence: 99%

Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador

et al. 2020

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Antimicrobial resistance (AMR) is a major health threat for public and animal health in the twenty-first century. In Ecuador, antibiotics have been used by the poultry industry for decades resulting in the presence of multi-drug resistant (MDR) bacteria in the poultry meat production chain, with the consequent risk for public health. This study evaluated the prevalence of ESBL/AmpC and mcr genes in third-generation cephalosporin-resistant Escherichia coli (3GC-R E. coli) isolated from broiler farms (animal component), broiler carcasses (food component), and human enteritis (human component) in Quito-Ecuador. Samples were collected weekly from November 2017 to November 2018. For the animal, food, and human components, 133, 335, and 302 samples were analyzed, respectively. Profiles of antimicrobial resistance were analyzed by an automated microdilution system. Resistance genes were studied by PCR and Sanger sequencing. From all samples, 122 (91.7%), 258 (77%), and 146 (48.3%) samples were positive for 3GC-R E. coli in the animal, food, and human components, respectively. Most of the isolates (472/526, 89.7%) presented MDR phenotypes. The ESBL blaCTX-M-55, blaCTX-M-3, blaCTX-M-15, blaCTX-M-65, blaCTX-M-27, and blaCTX-M-14 were the most prevalent ESBL genes while blaCMY-2 was the only AmpC detected gene. The mcr-1 gene was found in 20 (16.4%), 26 (10.1%), and 3 (2.1%) of isolates from animal, food, and human components, respectively. The implication of poultry products in the prevalence of ESBL/AmpC and mcr genes in 3GC-R must be considered in the surveillance of antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador

et al. 2020

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“…Moreover, mobile genetic elements (MGEs) can facilitate the spread of bla NDM genes, and the similarity of the bla NDM -flanking sequences in these plasmids suggests that horizontal mobilization of bla NDM via MGEs is responsible for its evolution and rapid transmission between plasmids and chromosomes ( 8 – 10 ). Furthermore, insertion sequences, such as IS Aba125 , IS 3000 , IS 26 , IS 5 , IS CR1 , Tn 3 , Tn 125 , Tn 3000 , and Tn 1548 , seem to play an important role in the dissemination of NDM-encoding genes ( 8 , 11 – 13 ). Most of the bla NDM sequences available in GenBank are flanked upstream by a complete or truncated copy of IS Aba125 and downstream by the ble MBL gene.…”

Section: Introductionmentioning

confidence: 99%

IS 26 Is Responsible for the Evolution and Transmission of bla _NDM -Harboring Plasmids in Escherichia coli of Poultry Origin in China

et al. 2021

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Carbapenem resistance in members of the order Enterobacterales is a growing public health problem that is associated with high mortality in developing and industrialized countries. Moreover, in the field of veterinary medicine, the occurrence of New Delhi metallo-β-lactamase-producing Escherichia coli isolates in animals, especially food-producing animals, has become a growing concern in recent years.

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“…Clonal spread in Brazil 65,66 and certain South American countries has been extensive 64,67 . Reyes et al reviewed publications (in English or Spanish) until 2019; data from seven South American countries noted that K. pneumoniae belonging to clonal complex 258 was most common; the most prevalent CPase genes were KPC and NDM 68 . A recent study in a hospital in Brazil reported 27 isolates of CP-resistant K. pneumoniae (CR-Kp); 24 (88.8%) produced KPC; 16 (59.2%) produced NDM; 13 (48.1%) expressed both genes 65 .…”

Section: Carbapenemases (Cpases) and International Spreadmentioning

confidence: 99%

Escalating antimicrobial resistance among Enterobacteriaceae: focus on carbapenemases

Lynch¹,

Clark

Zhanel

2021

Expert Opinion on Pharmacotherapy

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aminoglycosides (AG); antimicrobial resistance (AMR); -lactamase inhibitors (BLI); blood stream infections (BSIs) ; carbapenem (CP); carbapenemase (CPase); carbapenem-resistant (CR); carbapenemresistant Enterobacterales (CRE) ;-lactam inhibitor (BLI); CP-resistant K. pneumoniae (CRKP); CPsusceptible K. pneumoniae (CSKP); ceftazidime/avibactam (CAZ-AVI); cephalosporins (CEPHS); central line-associated (CLA); central venous catheter (CVC); community-acquired infection (CAI); complicated intra-abdominal infection (c-IAI); complicated urinary tract infection (c-UTI); defined daily dose (DDD) ; extended-spectrum -lactamases (ESBLs); extensively Drug Resistant (eXDR); fluoroquinolones (FQ); Gram negative bacteria (GNB); gross domestic product per capita (GDPPC); high-income countries (HICs); hospital acquired infection (HAI); imipenem-relebactam (IMI-REL); intensive care units (ICUs); Klebsiella pneumoniae carbapenemases (KPCs); long-term care facilities (LTCFs); low-and middleincome countries (LMICs); mechanical ventilation; metallo-lactamase (MBL); minimal inhibitory concentration (MIC); mobile genetic elements (MGEs); multidrug resistant (MDR); New Delhi Metalloproteinase-1 (NDM-1); non-Proteus Enterobacterales (NPE); organ transplant recipients (OTR); piperacillin/tazobactam (PTZ); solid organ transplant (SOT); tigecycline (TGC); ventilator-associated pneumonia (VAP)

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Mobile genetic elements associated with carbapenemase genes in South American Enterobacterales

Cited by 31 publications

References 56 publications

Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador

Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador

IS 26 Is Responsible for the Evolution and Transmission of bla _NDM -Harboring Plasmids in Escherichia coli of Poultry Origin in China

Escalating antimicrobial resistance among Enterobacteriaceae: focus on carbapenemases

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Mobile genetic elements associated with carbapenemase genes in South American Enterobacterales

Cited by 31 publications

References 56 publications

Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador

Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador

IS 26 Is Responsible for the Evolution and Transmission of bla NDM -Harboring Plasmids in Escherichia coli of Poultry Origin in China

Escalating antimicrobial resistance among Enterobacteriaceae: focus on carbapenemases

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IS 26 Is Responsible for the Evolution and Transmission of bla _NDM -Harboring Plasmids in Escherichia coli of Poultry Origin in China