Mobile Phone Microscopy for the Diagnosis of Soil-Transmitted Helminth Infections: A Proof-of-Concept Study

Bogoch, Isaac I.; Andrews, Jason R; Speich, Benjamin; Utzinger, Jürg; Shaali, M.; Ali, Said M.; Keiser, Jennifer

doi:10.4269/ajtmh.12-0742

Cited by 103 publications

(93 citation statements)

References 17 publications

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“…17,18 Newer models of handheld and mobile-phonebased microscopes have recently been designed and evaluated for several infections with global health significance, including malaria, schistosomiasis, soil-transmitted helminthiasis, giardiasis, and Loa loa. [8][9][10]12,13,19,20 To date, most of these studies have been conducted in controlled laboratory settings, and the few field studies conducted used expert microscopists to operate novel diagnostic tools. Digitization of images (in this case, with a mobile phone camera) may be useful for several reasons, including integrating machine learning software for automated identification and quantification of pathogens, or for automated diseases mapping with geographic information systems.…”

Section: Discussionmentioning

confidence: 99%

“…Handheld microscopy and mobile phone microscopy have recently been evaluated to overcome some of the logistic issues with laboratory-based diagnostics in resource-constrained settings. [8][9][10][11][12][13] The aim of these compact diagnostic tools is to provide portable quality laboratory testing in rural, remote, or underserviced settings rather than transferring ill individuals (or specimens) to distant reference laboratories. The goal of this study was to evaluate the "real-world" diagnostic operating characteristics of a handheld light microscope with mobile phone attachment integrated into a communitybased screening program for malaria in rural Côte d'Ivoire.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire

Coulibaly

Ouattara

Keiser

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Portable microscopy may facilitate quality diagnostic care in resource-constrained settings. We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of Plasmodium falciparum in a cross-sectional study in rural Côte d'Ivoire. Single Giemsa-stained thick blood film from 223 individuals were prepared and read by local laboratory technicians on both microscopes under 1,000× magnification with oil. Of the 223 samples, 162 (72.6%) were P. falciparum positive, and the overall mean parasite count was 1,392/μL of blood. Sensitivity and specificity of the handheld microscope was 80.2% (95% confidence interval [CI]: 73.1-85.9%) and 100.0% (95% CI: 92.6-100.0%), respectively, with a positive and negative predictive value of 100.0% (95% CI: 96.4-100.0%) and 65.6% (95% CI: 54.9-74.9%), respectively. If sensitivity can be improved, handheld light microscopy may become a valuable public health tool for P. falciparum diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire

Coulibaly

Ouattara

Keiser

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…[1][2][3][4] Recently, handheld and mobile phone-based microscopes have been used in public health settings to screen for endemic parasitic diseases, such as malaria, schistosomiasis, and soil-transmitted helminthiasis. [5][6][7][8][9][10] Such devices may improve access to laboratory diagnoses for clinical and public health practices in resource-constrained settings given their portability and ease of use, thus facilitating point-of-care diagnosis and subsequent patient management.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Opisthorchis viverrini Infection with Handheld Microscopy in Lao People's Democratic Republic

Bogoch¹,

Sayasone²,

Vonghachack³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Opisthorchiasis is a neglected tropical disease, yet it is of considerable public health importance in Southeast Asia given the predilection for chronically infected persons to develop cholangiocarcinoma. We evaluated a handheld microscope for the diagnosis of Opisthorchis viverrini in a community-based setting in Lao People's Democratic Republic in comparison with conventional light microscopy. In stool samples collected from 104 individuals, handheld microscopy revealed a sensitivity of 70.6% and a specificity of 89.5% for O. viverrini infection. Pearson's correlation for quantitative fecal egg counts between the two devices was 0.98 (95% confidence interval: 0.98-0.99). With small adjustments to further increase diagnostic sensitivity, a handheld microscope may become a helpful tool to screen for O. viverrini and other helminth infections in public health settings.

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“…Although these approaches demonstrated high-resolution imaging, they typically lack the flexibility and versatility of a stereo microscope where the user can easily adjust the focus and magnification or the illumination conditions depending on the sample that is being imaged. An add-on optical module, e.g., a lens attachment for high-resolution imaging [21,[31][32][33][34], would be an attractive solution in order to maintain the intended generic use of a stereo-microscope.…”

Section: Introductionmentioning

confidence: 99%

An Optofluidic Lens Array Microchip for High Resolution Stereo Microscopy

2014

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Abstract:We report the development of an add-on, chip-based, optical module-termed the Microfluidic-based Oil-immersion Lenses (μOIL) chip-which transforms any stereo microscope into a high-resolution, large field of view imaging platform. The μOIL chip consists of an array of ball mini-lenses that are assembled onto a microfluidic silicon chip. The mini-lenses are made out of high refractive index material (sapphire) and they are half immersed in oil. Those two key features enable submicron resolution and a maximum numerical aperture of ~1.2. The μOIL chip is reusable and easy to operate as it can be placed directly on top of any biological sample. It improves the resolution of a stereo microscope by an order of magnitude without compromising the field of view; therefore, we believe it could become a versatile tool for use in various research studies and clinical applications.

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Mobile Phone Microscopy for the Diagnosis of Soil-Transmitted Helminth Infections: A Proof-of-Concept Study

Cited by 103 publications

References 17 publications

Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire

Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire

Diagnosis of Opisthorchis viverrini Infection with Handheld Microscopy in Lao People's Democratic Republic

An Optofluidic Lens Array Microchip for High Resolution Stereo Microscopy

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