Mobility Inhibition and Nematocidal Activity of Asarone and Related Phenylpropanoids on Second-Stage Larvae of Toxocara canis.

Sugimoto, Naoki; Goto, Yoshihisa; Akao, Nobuaki; Kiuchi, Fumiyuki; Kondo, Kaoru; Tsuda, Yoshisuke

doi:10.1248/bpb.18.605

Cited by 16 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, we demonstrated that β‐asarone decreases the growth of Sf9 cells in a dose‐dependent manner (Figure ). The toxic effects of β‐asarone are not restricted to insect cells but extend to other organisms including some plant pathogenic fungi (Lee, Lee, Yun, & Hwang, ), nematodes such as Toxocara canis (Sugimoto et al, ), and gram‐positive bacteria (McGaw, Jäger, van Staden, & Eloff, ). These findings thus suggest that the toxicologic target of β‐asarone is a common process conserved over a broad variety of species.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of β‐asarone on Sf9 insect cells

Yooboon

Kuramitsu

Bullangpoti

et al. 2019

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

β-Asarone is the predominant component of the essential oil of rhizomes of Acorus calamus Linn ( Sweet flag). Although rhizome extracts from this plant have long been used for insect pest control, their cytotoxic effects on insect cells are not well understood. In this study, we evaluated the potency of β-asarone as a natural insecticide by using a Spodoptera frugiperda cell line (Sf9). To assess the cytotoxic effects of β-asarone on Sf9 cells, we observed morphologic changes in treated cells and performed a cell proliferation assay and a DNA fragmentation assay. After 24 and 48 h of treatment with β-asarone, the proliferation of the Sf9 cells was inhibited in a dose-dependent manner, with IC 50 values of 0.558 mg/ml at 24 h and 0.253 mg/ml at 48 h. Morphologic changes in β-asarone-treated cells were typical of apoptosis and included loss of adhesion, cell shrinkage, and small apoptotic bodies. The DNA laddering present in β-asarone-treated SF9 cells and annexin V assay confirmed that this compound can induce apoptosis in insect cells.Together, these findings suggest that apoptosis induction may be one mechanism through which β-asarone inhibits the proliferation of insect cells and thus exerts insecticidal effects. K E Y W O R D S apoptosis, botanical pesticide, cell toxicity, Sf9, β-asarone

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of β‐asarone on Sf9 insect cells

Yooboon

Kuramitsu

Bullangpoti

et al. 2019

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…It is also used as the antifungal agent (Lee et al, 2004), anthelmintic or nematocide (Sugimoto et al, 1995). To our knowledge, the action of β-asarone in colon cancer cell lines has never been studied.…”

Section: Discussionmentioning

confidence: 99%

Beta-asarone Induces LoVo Colon Cancer Cell Apoptosis by Up-regulation of Caspases through a Mitochondrial Pathway in vitro and in vivo

Zou¹,

Liu²,

Zhou³

et al. 2012

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Beta-asarone is one of the main bioactive constituents in traditional Chinese medicine Acorus calamu. Previous studies have shown that it has antifungal and anthelmintic activities. However, little is known about its anticancer effects. This study aimed to determine inhibitory effects on LoVo colon cancer cell proliferation and to clarify the underlying mechanisms in vitro and in vivo. Dose-response and time-course anti-proliferation effects were examined by MTT assay. Our results demonstrated that LoVo cell viability showed dose-and time-dependence on β-asarone. We further assessed anti-proliferation effects as β-asarone-induced apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide assay usinga flow cytometer and observed characteristic nuclear fragmentation and chromatin condensation of apoptosis by microscopy. Moreover, we found the apoptosis to be induced through the mitochondrial/caspase pathway by decreasing mitochondrial membrane potential (MMP) and reducing the Bcl-2-to-Bax ratio, in addition to activating the caspase-9 and caspase-3 cascades. Additionally, the apoptosis could be inhibited by a pan-caspase inhibitor, carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK). When nude mice bearing LoVo tumor xenografts were treated with β-asarone, tumor volumes were reduced and terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assays of excised tissue also demonstrated apoptotic changes. Taken together, these findings for the first time provide evidence that β-asarone can suppress the growth of colon cancer and the induced apoptosis is possibly mediated through mitochondria/caspase pathways.

show abstract

“…The essential oil from the rhizomes is also used in production of beer and alcoholic beverages such as bitters, liqueurs, vermouths and at lower level in foods such as frozen desserts, yoghurts, cakes and confectionery (Raina et al, 2003). The rhizome contains active constituents possessing insecticidal properties (Singh & Upadhyay, 1993;Schmidt & Streloke, 1994;Perrett & Whitfield, 1995;Sugimoto et al, 1995;Paneru et al, 1997;Rham & Schmidt, 1999;Raina et al, 2003;Lahlou, 2004). However, to the best of our knowledge, little work has been done on considering its insecticidally active constituents.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of insecticidal activity of (Z)‐asarone from Acorus calamus L.

et al. 2008

View full text Add to dashboard Cite

The insecticidal activity of Acorus calamus L. rhizome‐derived material against adults of Sitophilus zeamais Motschulsky was examined by using repellency method and contact toxicity. The biologically active constituent of the A. calamus rhizome was separated and identified. The results showed that the ethanol extract of A. calamus had strong repellency and contact effect to S. zeamais and the active constituent of the A. calamus was characterized as (Z)‐asarone by spectroscopic analysis. Responses from the tests varied with exposure times and doses. In the repellency test, ethanol extract of A. calamus had 93.92% repellency at 629.08 μg/cm2 but only 71.38% at 157.27 μg/cm2 12 h after treatment. As a contrast, (Z)‐asarone showed 84.50% repellency at 314.54 μg/cm2 and 77.02% at 78.63 μg/cm2 12 h after treatment. In the filter paper diffusion test, ethanol extract of A. calamus caused 95.56% and 17.78% mortality to S. zeamais at 314.54 μg/cm2 and 78.63 μg/ cm2 4 days after treatment, while (Z)‐asarone brought about 100.00% and 15.56% mortality at 40.89 μg/cm2 and 15.73 μg/cm2 respectively. These results indicate that the insecticidal activity of the A. calamus extract may be due to (Z)‐asarone.

show abstract

Mobility Inhibition and Nematocidal Activity of Asarone and Related Phenylpropanoids on Second-Stage Larvae of Toxocara canis.

Cited by 16 publications

References 0 publications

Cytotoxic effects of β‐asarone on Sf9 insect cells

Cytotoxic effects of β‐asarone on Sf9 insect cells

Beta-asarone Induces LoVo Colon Cancer Cell Apoptosis by Up-regulation of Caspases through a Mitochondrial Pathway in vitro and in vivo

Isolation and characterization of insecticidal activity of (Z)‐asarone from Acorus calamus L.

Contact Info

Product

Resources

About