Model for the amperometric enzyme electrode obtained through digital simulation and applied to the immobilized glucose oxidase system

Mell, Leroy D.; Maloy, J. T.

doi:10.1021/ac60352a006

Cited by 299 publications

(127 citation statements)

References 34 publications

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“…The response of the electrode exhibits characteristics of the Michaelis-Menten kinetic mechanism. The apparent Michaelis-Menten constant, Km, of tyrosinase on the tyrosinase, NG-MTP-Au electrode to substrate can be calculated according to the Lineweaver-Burk equation, 9,[17][18][19] …”

Section: Amperometric Resultsmentioning

confidence: 99%

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

et al. 2006

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Many studies on the use of enzymatic activity in nonaqueous solutions have been carried out after Dastoli and Price's report of their investigation of the catalytic activity of xanthine oxidase suspended in several organic media. 1 Updike and Hicks published the results of their experiment involving an enzyme electrode with cross-linked polyacrylamide (PAA) membrane as matrix for use in aqueous solutions.2 Enzymes have been found to have numerous applications as practical and specific catalysts in chemical and pharmaceutical syntheses and as recognition elements in biosensors. The discovery that they are catalytically active in organic solvents containing little or no water has expanded their repertoire of use still further. 3,4 Some of the benefits in utilizing enzymes in nonaqueous solvents over the use of conventional aqueous reaction media include the high solubility of many hydrophobic substrates in such solvents, the suppression of various side reactions promoted by water, altered enzymatic selectivity and the ability to control it with the solvent, and the comparative simplicity of immobilization procedures due to the insolubility of enzymes in organic solvents. Many studies have been conducted in order to gain an understanding of so-called solvent effects due to organic solvent properties. Factors such as solvent hydrophobicity, 5 solvent polarity, water content, 6 and substrate hydrophobicity 7 can affect the enzymatic reaction. It remains important to continue the search for new enzymatic reaction systems because of the use which might be made of such systems in both pure and applied research.Many enzyme-based electrochemical biosensors have been described for use in both aqueous and nonaqueous solutions. A tyrosinase-immobilized polyacrylamide membrane platinum electrode has been prepared and the electrochemical behavior of o-hydroxybenzene derivatives in acetonitrile 8 and dimethylacetamide 9 has been studied. Because of unique properties of nanoparticles, recent investigations have focused on the development of enzyme-modified nanoparticle-based electrochemical biosensors. [10][11][12] The ability of nanoparticles to stabilize enzymes, and to improve the efficiency of immobilized enzymes is extremely useful when preparing biosensors. Moreover, other characteristics of nanoparticles, such as their high surface-to-volume ratio, their high surface energy, and their ability to decrease the distance between enzyme and metal particles, may facilitate electron transfer between redox sites and the electrode surfaces.In our laboratory, a gold nanoparticle self-assembled electrode has been constructed by using 4,4′-bis(methanethiol)biphenyl (MTP), rigid rod dithiols, as a binder between a gold disk and gold nanoparticles. The effect of gold nanoparticles on the interaction of Co(phen)3 3+ with DNA, and the characteristics of the electrode in a nonaqueous solution were described in a recent report. 13 We have also devised a method of preparing density-controlled gold nano-particle self-assembled monolayer (SAM...

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Section: Amperometric Resultsmentioning

confidence: 99%

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

et al. 2006

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“…The flattening of the calibration curves at higher concentrations is dictated by Michaelis-Menten kinetics. It is possible to see that at lower levels the enzyme reaction approximates the first order kinetics whereas at highest concentrations the reaction order approaches zero and the measured current becomes independent of glucose (enzyme substrate) concentration (Mell & Maloy, 1974) The wide measured concentration range and extremely low limit of detection is the result of nanostructure as mentioned in section 1. The immobilization is made by this manner that www.intechopen.com …”

Section: Measurement Of Glucose Oxidase By Electrochemical Sensor Witmentioning

confidence: 90%

Screen printed electrodes improve mass transfer

Šejnohová¹,

Hanák²,

Krejčí³

2011

New Perspectives in Biosensors Technology and Applications

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“…The apparent Michaelis-Menten constant, K m, a reflection of enzymatic affinity, can be calculated according to the Lineweaver-Burk equation ([Mell and Maloy, 1975], [Shu and Wilson, 1976] and [Kamin and Wilson, 1980]). …”

Section: Amperometric Detection Of Superoxide Radicalmentioning

confidence: 99%

A sensor for superoxide in aqueous and organic/aqueous media based on immobilized cytochrome c on binary self-assembled monolayers

Ren

Jin

et al. 2007

Biosensors and Bioelectronics

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A method for the electrochemical detection of superoxide radical was developed, based on cytochrome c (cyt c) immobilized on the binary self-assembled monolayers (SAMs) of thioctic acid (T-COOH) and thioctic amide (T-NH 2 ) on gold electrode. The sensor works by electrochemically detecting cyt c reduced by the superoxide radical generated by a xanthine-XOD system. The electrochemical properties of immobilized cyt c were investigated in aqueous buffer and in a mixture of aqueous and organic solvents. The interaction of superoxide radical with the modified electrode was characterized in phosphate buffer solution (PBS) and in the mixtures of both PBS and dimethyl sulfoxide (DMSO) and PBS and glycerol (Gly). The results showed that the sensors responded immediately to superoxide radical in PBS and gave a steady-state anodic current within 10 s during the generation of superoxide radical. In 40% DMSO and in 30% Gly solution, the current response reached a steady-state anodic current within 20 s. The sensor could also be used to estimate superoxide dismutase (SOD).

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Model for the amperometric enzyme electrode obtained through digital simulation and applied to the immobilized glucose oxidase system

Cited by 299 publications

References 34 publications

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

Screen printed electrodes improve mass transfer

A sensor for superoxide in aqueous and organic/aqueous media based on immobilized cytochrome c on binary self-assembled monolayers

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