Modeling and Forecasting Vibrio Parahaemolyticus Concentrations in Oysters

Namadi, Peyman; Deng, Zhiqiang

doi:10.1016/j.watres.2020.116638

Cited by 13 publications

(3 citation statements)

References 37 publications

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“…Vibrios are naturally occurring halophilic bacteria that can accumulate in oysters when they filter water [ 18 ]. Thus, exposure to vibrios is a frequent cause of seafood-borne illnesses [ 19 , 20 , 21 ]. Vibrio densities vary with environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…We and others have demonstrated that vibrio concentrations are higher during warm months and lower during colder months [ 5 , 12 , 22 ]. Their densities are also related to salinity, chlorophyll, turbidity, and other factors [ 5 , 6 , 20 , 23 , 24 , 25 , 26 , 27 , 28 ]. Because vibrios naturally occur in marine and estuarine environments, the most effective way to minimize infections is to minimize exposure to these bacteria when their concentrations are high.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Alternative Colony Hybridization Methods for Pathogenic Vibrios

Schwartz

Marcotte

Johnson

2023

Foods

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Vibrios, such as Vibrio parahaemolyticus, are naturally occurring halophilic bacteria that are a major cause of foodborne illness. Because of their autochthonous nature, managing vibrio levels in marine and estuarine environments is impossible. Instead, it is crucial to reliably enumerate their abundance to minimize human exposure. One method of achieving this is the direct plating/colony hybridization (DP/CH) method, which has been used to efficiently quantify pathogenic vibrios in oysters and other seafood products. Although successful, the method relies on proprietary resources. We examined alternative approaches, assessed the influence of the reagent suppliers’ source on enumeration accuracy, and made experimental adjustments that maximized efficiency, sensitivity, and specificity. We report here that in-house conjugation via Cell Mosaic is a viable alternative to the previously available sole-source distributor of the alkaline phosphatase-conjugated probes used to enumerate vibrios in oysters. We also report that milk was a viable alternative as a blocking reagent, pH must be eight, an orbital shaker was a viable alternative to a water bath, and narrow polypropylene containers were a viable alternative to Whirl-Pak bags. These modifications will be crucial to scientists enumerating vibrios and other pathogens in food products.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Alternative Colony Hybridization Methods for Pathogenic Vibrios

Schwartz

Marcotte

Johnson

2023

Foods

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“…Previous findings in the PNW have laid the groundwork to characterize space–time residual dependencies for the relationships between environmental measures and V. parahaemolyticus abundance in the region ( Paranjpye et al, 2015 ; Flynn et al, 2019 ). Statistical assessments accounting for spatial heterogeneity across the varied aquatic “zones” found in Washington state and sampling autocorrelation are also important to consider ( Davis et al, 2021 ; Namadi and Deng, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters

et al. 2022

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The Pacific Northwest (PNW) is one of the largest commercial harvesting areas for Pacific oysters (Crassostrea gigas) in the United States. Vibrio parahaemolyticus, a bacterium naturally present in estuarine waters accumulates in shellfish and is a major cause of seafood-borne illness. Growers, consumers, and public-health officials have raised concerns about rising vibriosis cases in the region. Vibrio parahaemolyticus genetic markers (tlh, tdh, and trh) were estimated using an most-probable-number (MPN)-PCR technique in Washington State Pacific oysters regularly sampled between May and October from 2005 to 2019 (N = 2,836); environmental conditions were also measured at each sampling event. Multilevel mixed-effects regression models were used to assess relationships between environmental measures and genetic markers as well as genetic marker ratios (trh:tlh, tdh:tlh, and tdh:trh), accounting for variation across space and time. Spatial and temporal dependence were also accounted for in the model structure. Model fit improved when including environmental measures from previous weeks (1-week lag for air temperature, 3-week lag for salinity). Positive associations were found between tlh and surface water temp, specifically between 15 and 26°C, and between trh and surface water temperature up to 26°C. tlh and trh were negatively associated with 3-week lagged salinity in the most saline waters (> 27 ppt). There was also a positive relationship between tissue temperature and tdh, but only above 20°C. The tdh:tlh ratio displayed analogous inverted non-linear relationships as tlh. The non-linear associations found between the genetic targets and environmental measures demonstrate the complex habitat suitability of V. parahaemolyticus. Additional associations with both spatial and temporal variables also suggest there are influential unmeasured environmental conditions that could further explain bacterium variability. Overall, these findings confirm previous ecological risk factors for vibriosis in Washington State, while also identifying new associations between lagged temporal effects and pathogenic markers of V. parahaemolyticus.

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Deep learning-based ensemble modeling of Vibrio parahaemolyticus concentration in marine environment

Namadi

Deng

2022

Environ Monit Assess

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Modeling and Forecasting Vibrio Parahaemolyticus Concentrations in Oysters

Cited by 13 publications

References 37 publications

Evaluation of Alternative Colony Hybridization Methods for Pathogenic Vibrios

Evaluation of Alternative Colony Hybridization Methods for Pathogenic Vibrios

Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters

Deep learning-based ensemble modeling of Vibrio parahaemolyticus concentration in marine environment

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