Modeling gradient elution of proteins in ion‐exchange chromatography

Abstract

The stenc mass action (SMA) model of ion exchange is employed in concert with

Binary protein adsorption on gel‐composite ion‐exchange media

“…The z-value determined for cytochrome-C is close to those reported by other authors Ž . Brooks and Cramer, 1992;Gallant et al, 1996 . The value of z for lysozyme, however, is smaller than the value reported Ž .…”

Unique selectivity windows using selective displacers/eluents and mobile phase modifiers on hydroxyapatite

“…A Jupiter 5 m C4 300A column (4.6 mm × 50 mm) was purchased from Phenomenex (Torrance, CA). Ribonuclease A from bovine pancreas (RNaseA), ribonuclease B from bovine pancreas (RNaseB), ␣-chymotrypsinogen A from bovine pancreas (␣-ChyA), cytochrome C from equine heart (CytC), lysozyme from chicken egg white (Lys), conalbumin from chicken egg white (Conal), hemoglobin from bovine blood (Hemo), myoglobin from equine heart (Myo), avidin from chicken egg white, subtilisin A from Bacillus, elastase from porcine pancreas, papain from papaya latex, bromelain from pineapple stem, alcohol dehydrogenase from equine liver, trypsinogen from bovine pancreas, catalase from bovine liver, aprotinin from bovine lung, aconitase from porcine heart, albumin from bovine serum, neomycin sulfate (displacer 1), paromomycin sulfate (2), bekanamycin sulfate (3), amikacin sulfate (4), spermine (5), bis(hexamethylene)triamine (7), spermidine (8), 1,4-bis(3-aminopropyl)piperazine (9), diethylenetriamine (10), 4,7,10-trioxa-1,13-tridecanediamine (11), N,Ndiethyl-1,3-propanediamine (12), N,N-diethyldiethylenetriamine (13), 2-(2-aminoethylamino)ethanol (14), spectinomycin dihydrochloride pentahydrate (15), l-arginine methyl ester dihydrochloride (16), l-lysine methyl ester dihydrochloride (17), N-hexylethylenediamine (18), piperazine (19), cyclohexylamine (20), acetic acid (21), malonic acid (22), succinic acid (23), adipic acid (24), isocitric acid lactone (25), trans-aconitic acid (26), 1,2,4-butanetricarboxylic acid (27), 1,2,3,4-butanetetracarboxylic acid (28), glycine (29), 3-guanidinopropionic acid (30), 5-aminovaleric acid (31), pantothenic acid (32), aspartic acid (33), l-␤-homoglutamic acid hydrochloride (34), guanidinosuccinic acid (35), l-2,3-diaminopropionic acid hydrochloride (36), lysine (37), arginine (38), meso-2,3,-diaminosuccinic acid (39), ethylenediaminetetrapropionic acid (40), glycerol (41), threitol (42), adonitol (43), dulcitol (44), malic acid (45), tartaric acid (46), mucic acid …”

“…Mass action selective displacers typically have an affinity for the resin that lies between that of the two solutes being separated and can be readily predicted using the steric mass action formalism [23,24]. While selective displacement chromatography has been successfully employed in ion exchange systems [5,9,11,[14][15][16][17][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], to date it has not been applied to CHA or any other multi-modal resin system.…”

Elution relationships to model affinity chromatography using a general rate model

“…Several papers can be found in the literature that present models to simulate protein elution curves of different types of chromatography (Yamamoto et al, 1983;Gallant et al, 1996;Li et al, 1998;Gu and Zheng, 1999;Gu et al, 2003;Shene et al, 2006;Lienqueo et al, 2009;Orellana et al, 2009). In particular, for affinity chromatography, there is work from Gu and coworkers (2003) who use a general rate model with a second-order kinetic binding reaction including size exclusion factors.…”