Modeling of batch experimental kinetics and application to fed-batch fermentation of Clostridium tyrobutyricum for enhanced butyric acid production

Song, Hyohak; Eom, Moon-Ho; Lee, Sira; Lee, Jung Hwa; Cho, Jung-Hee; Seung, Doyoung

doi:10.1016/j.bej.2010.09.010

Cited by 48 publications

(26 citation statements)

References 42 publications

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“…Cell mass concentrations reported for batch-and fed-batch cultivations using glucose as the carbon source and a similar basal salts medium (no peptones) as the present study range between 3 and 10.1 g/L (Jaros et al, 2013;Michel-Savin et al, 1990b). However, using reinforced clostridia medium, a dry cell concentration of 17 g/L has been reported for fed-batch cultivation (Song et al, 2010). The comparison therefore suggests that the growth promotive properties of the sorghum hydrolysate juice may be linked to its protein or amino acid content.…”

Section: Fermentation Using the Hydrolysate Juice As Feed-stockcontrasting

confidence: 52%

“…However, these fermentation results were obtained with glucose concentrations of up to 150 g/L which may severely prolong the initial lag phase (Michel-Savin et al, 1990b). In fed-batch fermentations using suspended cells and glucose as the carbon source, a butyrate concentration of 73 g/L, a productivity of 1.4 g/L h and a yield of 0.46 g/g have been reported (Song et al, 2010). With continuous systems, the productivity is generally higher but the butyrate and cell mass concentrations are lower.…”

Section: Introductionmentioning

confidence: 81%

“…The feed profile was empirically developed for non-limiting sugar feeding, and to avoid excessive sugar inhibition. The highest specific growth rate of C. tyrobutyricum has been reported to occur at a glucose concentration between 10 and 60 g/L (Song et al, 2010). In this interval, the specific growth rate was shown to vary only between 0.38 and 0.48 h −1 and the maximum occurred at 23.6 g/L of glucose.…”

Section: Feed Profile For the Hydrolysatementioning

confidence: 95%

“…Several different cultivation techniques have been investigated for butyrate production including batch, (Fayolle et al, 1990), fed-batch (Mitchell et al, 2009) and continuous systems (Du et al, 2014). Batch fermentations have been reported to give butyrate titers of up to 62.5 g/L, volumetric productivities of 0.99 g/L h and butyrate yields of 0.466 g/g with glucose as carbon source (Song et al, 2010). However, these fermentation results were obtained with glucose concentrations of up to 150 g/L which may severely prolong the initial lag phase (Michel-Savin et al, 1990b).…”

Section: Introductionmentioning

confidence: 99%

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Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Sjöblom

Μάτσακας

Christakopoulos

et al. 2015

Industrial Crops and Products

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a b s t r a c tEnzymatically liquefied sweet sorghum stalks and beet molasses were evaluated for butyrate production using Clostridium tyrobutyricum in 1 L scale fed-batch fermentations. The hydrolysates used for the fermentations were prepared separately by liquefying the sorghum stalks at 50 • C, pH 5.0 for 18 h, with 30% (w/v) DM content using the enzyme preparation Cellic ® CTec2 at an activity of 26.5 FPU/g DM. To enhance sucrose consumption, the fermentations were supplemented with invertase at an activity equivalent to 8.3 U/g DM. With the hydrolysate as the feedstock, a butyrate concentration of 37.2 ± 0.8 g/L, a productivity of 0.86 ± 0.02 g/L h and a yield of 0.39 ± 0.02 g/g (p = 0.05) consumed sugars were obtained. Finally, a butyrate concentration of 58.8 g/L, a productivity of 1.9 g/L h, a butyrate yield of 0.52 g/g consumed sugars and a dry cell mass concentration of 15.7 g/L were obtained with fed-batch cultivation and a constant feed consisting of 64% sorghum hydrolysate juice and 36% molasses. Evidence for inducible saccharolytic activity was also proven, as the cellulase activity in the culture supernatant was found more than double during feed with limiting sugar concentrations. The present study clearly demonstrates that combinations of low cost raw materials can be used for efficient butyrate production, also without cell immobilization.

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Section: Fermentation Using the Hydrolysate Juice As Feed-stockcontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 81%

Section: Feed Profile For the Hydrolysatementioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Sjöblom

Μάτσακας

Christakopoulos

et al. 2015

Industrial Crops and Products

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“…The characteristics of these Clostrium species are gram-positivity, chemo-organotrophy, strict anaerobiosity, and spore formation (Zigová et al 1999). Clostridium tyrobutyricum is one of the most promising strains due to its ability to produce butyric acid with high selectivity, the tolerance of high concentrations of products, and relatively high and stable productions (Zigová and Šturdík 2000;Liu and Yang 2006;Najafpour 2006;Song et al 2010). This strain is able to utilize some common carbon sources, such as glucose, xylose, fructose, and lactate (Dwidar et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Butyric Acid Fermentation in Xylose and Glucose by Clostridium tyrobutyricum

et al. 2017

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Adaptive evolution for fast growth on glucose and the effects on the regulation of glucose transport system in Clostridium tyrobutyricum

Jiang

et al. 2011

Biotech & Bioengineering

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Laboratory adaptive evolution of microorganisms offers the possibility of relating acquired mutations to increased fitness of the organism under the conditions used. By combining a fibrous-bed bioreactor, we successfully developed a simple and valuable adaptive evolution strategy in repeated-batch fermentation mode with high initial substrate concentration and evolved Clostridium tyrobutyricum mutant with significantly improved butyric acid volumetric productivity up to 2.25 g/(L h), which is the highest value in batch fermentation reported so far. Further experiments were conducted to pay attention to glucose transport system in consideration of the high glucose consumption rate resulted from evolution. Complete characterization and comparison of the glucose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) were carried out in the form of toluene-treated cells and cell-free extracts derived from both C. tyrobutyricum wide-type and mutant, while an alternative glucose transport route that requires glucokinase was confirmed by the phenomena of resistance to the glucose analogue 2-deoxyglucose and ATP-dependent glucose phosphorylation. Our results suggest that C. tyrobutyricum mutant is defective in PTS activity and compensates for this defect with enhanced glucokinase activity, resulting in the efficient uptake and consumption of glucose during the whole metabolism.

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Modeling of batch experimental kinetics and application to fed-batch fermentation of Clostridium tyrobutyricum for enhanced butyric acid production

Cited by 48 publications

References 42 publications

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Butyric Acid Fermentation in Xylose and Glucose by Clostridium tyrobutyricum

Adaptive evolution for fast growth on glucose and the effects on the regulation of glucose transport system in Clostridium tyrobutyricum

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