Modeling Tetrahymena thermophila growth and protease production

Coninck, Joëlle De; Verdier-Denantes, I; Duyme, Florent; Bouquelet, Stéphane; Dumortier, V.

doi:10.1038/sj.jim.7000013

Cited by 7 publications

(8 citation statements)

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“…However, an increased biomass was obtained when aeration was 2 vvm. In the same way, aeration and agitation were previously found to influence growth and protease production (DeConink et al, 2000 and.…”

Section: Response Surfaces Methodolgy (Rsm)mentioning

confidence: 87%

Optimization of Extracellular Lipase Production by Penicillium chrysogenum Using Factorial Design

Shafei¹,

Mohamed²,

Elsalam³

2011

MJM

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The effect of oxygen on lipase production by Penicillium chrysogenum was studied under two operating modes, controlled aeration rate tested and controlled agitation at dissolved oxygen concentration (DO) 1.00 vvm. Lipase production and cell dry weight were tested in a stirred batch fermenter 5 L. Improvement in oxygen transfer rate (OTR) either by aeration or agitation resulted in an increase in lipase production. Growth curves and lipase activities of P.chrysogenum were examined at agitation rates (200,400,600 rpm), aeration rates (2,4 vvm) at different fermentation periods (24,48,72,96,120 h). Response Surface Methodology (RSM) using Design Expert software was used to study the effect of aeration, agitation, and fermentation time on lipase activity and cell dry weight. These factors were analyzed using 2 1 . 3 2 level factorial design. An optimal set of conditions that maximize lipase production: (2 vvm aeration; 600 rpm agitation after 72 h) was obtained. The maximum lipase activity obtained was 240 U/mL. Beside lipase activity, this paper also studies the optimal combination of the controllable factors (aeration; agitation and fermentation time) that will maximize the cell dry weight.

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Section: Response Surfaces Methodolgy (Rsm)mentioning

confidence: 87%

Optimization of Extracellular Lipase Production by Penicillium chrysogenum Using Factorial Design

Shafei¹,

Mohamed²,

Elsalam³

2011

MJM

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“…However, our results showed an improvement of biomass when oxygen was regulated at 50%. In the same way, aeration and agitation influenced growth and protease production, and have been previously optimised [7].…”

Section: Resultsmentioning

confidence: 97%

“…Aeration and agitation were not tested because their influence on growth and protease production has been optimised previously [7].…”

Section: Choice Of Factorsmentioning

confidence: 99%

Factorial designs: an efficient approach to choosing the main factors influencing growth and hydrolase production by Tetrahymena thermophila

Coninck

Leclercq

Exbrayat

et al. 2004

J IND MICROBIOL BIOTECHNOL

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A fractional factorial design with eight trials was applied to select and model the effects of major factors, individually and in combination, on improving Tetrahymena thermophila growth and enzyme production. Regulated pH at 6.8 and olive oil at 0.5% (v/v) showed positive effects on fermentation, and increased cell growth parameters including generation time and maximal population formation. Lipase and protease production were also improved by these factors and were favoured by cultivation of Tetrahymena in darkness. This statistical experiment offers a beneficial and rapid screening procedure to select the most effective combination of factors influencing fermentation processes.

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“…This was attributed to the loss of amino acids during fish powder preparation [179]. On the other hand, yeast extract together with 0.5 % glucose supported ciliate densities as high as 1.7 9 10 6 cells/mL in 100 L fermentors (bio-reactors) at 28°C [189]. Other milestones in Tetrahymena scale up have been the use of a defined medium and of an airlift reactor with a working volume of 1,200 L [195].…”

Section: Scaling Up Cultures Of Freshwater Ciliatesmentioning

confidence: 99%

“…The mass cultivation or scale up of freshwater ciliates has been done primarily to produce commercially important enzymes [189][190][191], but the methods might also be used to produce ciliates for use as fish larval feed. Large-scale production has been explored with T. thermophila, T. pyriformis, and T. rostrata [192].…”

Section: Scaling Up Cultures Of Freshwater Ciliatesmentioning

confidence: 99%

Use of Cell Cultures to Study the Interactions of Ciliates with Fish

Pinheiro¹,

Bols²

2013

Springer Science Reviews

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How the ciliates of fish can be cultured and be used to study ciliate/fish interactions are reviewed. The culturing of ciliates is currently done in basal solutions based on either freshwater, seawater, or bodily fluids of vertebrates. These are supplemented either with bacteria, fish cells, or organic matter, which can be defined or more commonly undefined, with proteose peptone being a prominent example. Among pathogenic ciliates, the most difficult to culture has been Ichthyophthirius multifiliis. To contrast, Cryptocaryon irritans caused a similar disease and has been maintained successfully in co-cultures with fish cells. Pathogenic scuticociliates and tetrahymenas are more amenable to culture, and can be grown axenically. These cultures have been used to study pathogenic mechanisms and to screen drugs for their potential chemotherapeutic value. Ciliates may act directly on fish to cause disease, but may also influence fish health indirectly through their interactions with other types of fish pathogens, for example bacteria and fungi. For example, in culture Tetrahymena spp. have been shown to phagocytose pathogenic bacteria, Yersinia ruckerii, and microsporidia, Glugea hertwigi, where ciliates are not linked to disease, very different ciliate/fish interactions are possible. For some fish larvae, free-living ciliates are a source of nutrients. Large-scale cultures of both freshwater and marine ciliates have been achieved and could be a source of feed for fish larvae in aquaculture. Finally, ciliates have the potential to feed on fish carcasses and in doing so make nutrients available to the ecosystem. In the future cell cultures should be invaluable in studying these and other possible relationships between fish and ciliates.

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Modeling Tetrahymena thermophila growth and protease production

Cited by 7 publications

References 0 publications

Optimization of Extracellular Lipase Production by Penicillium chrysogenum Using Factorial Design

Optimization of Extracellular Lipase Production by Penicillium chrysogenum Using Factorial Design

Factorial designs: an efficient approach to choosing the main factors influencing growth and hydrolase production by Tetrahymena thermophila

Use of Cell Cultures to Study the Interactions of Ciliates with Fish

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