Modeling the kinetics of vinyl chloride cometabolism by an ethane-grownPseudomonas sp.

Verce, Matthew F.; Freedman, David L.

doi:10.1002/1097-0290(2000)71:4<274::aid-bit1017>3.0.co;2-z

Cited by 23 publications

(21 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preferential use of a growth substrate prior to consumption of a nongrowth substrate is different from the more common pattern of simultaneous consumption of growth substrate and nongrowth substrate. Another pattern, in which the nongrowth substrate is consumed preferentially to the growth substrate, has also been observed (16,41). Thus, it appears there is a spectrum of substrate and nongrowth substrate consumption patterns.…”

Section: Discussionmentioning

confidence: 99%

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Verce

Gunsch

Danko

et al. 2002

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

An aerobic enrichment culture was grown on vinyl chloride (VC) as the sole source of carbon and energy. In the absence of VC, the enrichment culture cometabolized cis-1,2-dichloroethene (cDCE) and, to a lesser extent, trans1,2-dichloroethene (tDCE), beginning with oxidation to the corresponding DCE-epoxides. When provided with VC (1.3 mM) and cDCE (0.2-0.3 mM), the enrichment culture cometabolized repeated additions of cDCE for over 85 days. Cometabolism of repeated additions of tDCE was also demonstrated but at a lower ratio of nongrowth substrate to VC. VC-grown Pseudomonas aeruginosa MF1 (previously isolated from the enrichment culture) also readily cometabolizes cDCE, with an observed transformation capacity (Tc,obs) of 0.82 micromol of cDCE/mg of total suspended solids (TSS). When provided with VC and cDCE, MF1 did not begin cometabolizing cDCE until nearly all of the VC was consumed. The presence of cDCE reduces the maximum specific rate of VC utilization. A kinetic model was developed that describes these phenomena via Monod parameters for substrate and nongrowth substrate, plus inactivation and inhibition coefficients. MF1 did not show any cometabolic activity on tDCE or trichloroethene and very limited activity on 1,1-DCE (Tc,obs = 2 x 10(-5) micromol/mg TSS). Above 40 microM, tDCE and TCE noticeably increased the maximum specific rate of VC utilization, even though neither compound was consumed during or after VC consumption. High concentrations of 1,1-DCE (950 microM) completely inhibited VC biodegradation. As there is currently no evidence for aerobic biodegradation of cDCE as a sole source of carbon and energy, the results of this study provide a potential explanation for in situ disappearance of cDCE when the only other significant substrate available is VC. It is fortuitous that the VC-grown cultures tested exhibit their highest cometabolic activity toward cDCE, because it is the predominant DCE isomer formed during anaerobic reductive dechlorination of trichloroethene and tetrachloroethene.

show abstract

Section: Discussionmentioning

confidence: 99%

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Verce

Gunsch

Danko

et al. 2002

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, this concept has yet to be validated by pure culture or in situ studies. Earlier studies (Verce & Freedman, 2000; Freedman et al ., 2001) using ethene‐assimilating enrichments and an ethane‐assimilating Pseudomonas sp. found that addition of VC was not inhibitory to ethene degradation, but completely inhibited ethane degradation.…”

Section: Introductionmentioning

confidence: 92%

Aerobic biodegradation of the chloroethenes: pathways, enzymes, ecology, and evolution

2010

View full text Add to dashboard Cite

Extensive use and inadequate disposal of chloroethenes have led to prevalent groundwater contamination worldwide. The occurrence of the lesser chlorinated ethenes [i.e. vinyl chloride (VC) and cis-1,2-dichloroethene (cDCE)] in groundwater is primarily a consequence of incomplete anaerobic reductive dechlorination of the more highly chlorinated ethenes (tetrachloroethene and trichloroethene). VC and cDCE are toxic and VC is a known human carcinogen. Therefore, their presence in groundwater is undesirable. In situ cleanup of VC- and cDCE-contaminated groundwater via oxidation by aerobic microorganisms is an attractive and potentially cost-effective alternative to physical and chemical approaches. Of particular interest are aerobic bacteria that use VC or cDCE as growth substrates (known as the VC- and cDCE-assimilating bacteria). Bacteria that grow on VC are readily isolated from contaminated and uncontaminated environments, suggesting that they are widespread and influential in aerobic natural attenuation of VC. In contrast, only one cDCE-assimilating strain has been isolated, suggesting that their environmental occurrence is rare. In this review, we will summarize the current knowledge of the physiology, biodegradation pathways, genetics, ecology, and evolution of VC- and cDCE-assimilating bacteria. Techniques (e.g. PCR, proteomics, and compound-specific isotope analysis) that aim to determine the presence, numbers, and activity of these bacteria in the environment will also be discussed.

show abstract

“…Monod kinetic constants were determined using an approach similar to ones reported for other volatile organics, including vinyl chloride, ethene, and ethane (35)(36)(37). In order to provide a consistent source of culture for the kinetic experiments, NB1 was maintained in an anoxic reactor operated in a semicontinuous, draw-and-fill mode, at a hydraulic retention time of 20 days.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of Chloromethane by Pseudomonas aeruginosa Strain NB1 under Nitrate-Reducing and Aerobic Conditions

Freedman

Swamy

Bell

et al. 2004

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Modeling the kinetics of vinyl chloride cometabolism by an ethane-grownPseudomonas sp.

Cited by 23 publications

References 60 publications

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Aerobic biodegradation of the chloroethenes: pathways, enzymes, ecology, and evolution

Biodegradation of Chloromethane by Pseudomonas aeruginosa Strain NB1 under Nitrate-Reducing and Aerobic Conditions

Contact Info

Product

Resources

About