Modelling as a tool of enzyme reaction engineering for enzyme reactor development

Vasić-Rački, Đurđa; Findrik, Zvjezdana; Presečki, Ana Vrsalović

doi:10.1007/s00253-011-3414-0

Cited by 44 publications

(40 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Applying the mechanistic model for its description would result with a number of parameters and it is hard to get intrinsic control of its correctness. The formal kinetic model includes the data‐driven or the empirical model which has fewer kinetic constants . Those constants can be evaluated independently and have a defined physical meaning.…”

Section: Resultsmentioning

confidence: 99%

2‐Deoxyribose‐5‐phosphate aldolase from Thermotoga maritima in the synthesis of a statin side‐chain precursor: characterization, modeling and optimization

Švarc

Blažević

Vasić-Rački

et al. 2019

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

BACKGROUND Producing statin side‐chains by sequential aldol condensation catalyzed by 2‐deoxyribose‐5‐phosphate aldolase (DERA) (EC 4.1.2.4) is the best‐known and most promising synthetic route. The advantage of this process is the formation of two stereocenters in a single step starting from inexpensive achiral components, acetaldehyde and chloroacetaldehyde. The limitation for the industrial‐scale application is enzyme inactivation caused by substrates as well by the intermediate produced by single aldol addition. RESULTS The DERA enzyme from Thermotoga maritima (DERATm) was investigated extensively in this work. The influence of aldehydes on DERATm stability was studied in detail. Mathematical models in different reactor configurations were developed based on experimentally determined kinetic parameters of all reactions included in the synthesis of statin side‐chain. Models also included enzyme inactivation by all compounds with negative impact on its stability. Mathematical model‐based optimization enabled optimization of process conditions and reactor configuration. The fed‐batch reactor proved to be the most suitable choice achieving product concentration of 78 g L−1, productivity of 56 g L day−1 and yield of 95% under an optimal condition. CONCLUSION The validated mathematical model and applied methodology can be exploited for further process improvement and for process design of similar systems. © 2019 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

show abstract

Section: Resultsmentioning

confidence: 99%

2‐Deoxyribose‐5‐phosphate aldolase from Thermotoga maritima in the synthesis of a statin side‐chain precursor: characterization, modeling and optimization

Švarc

Blažević

Vasić-Rački

et al. 2019

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

show abstract

“…This approach suffers the difficulty of producing a model that is both cumbersome and challenging to calibrate. Thus, an intermediate approach involving the use of simplified reactions that reflect the dominant reactions of the catalytic cycle along with the use of a minimum number of kinetic coefficients requiring calibration is particularly attractive when faced with reaction condition constraints and complex enzyme reaction systems [83]. This is the approach that has been used in the present study.…”

Section: Model Developmentmentioning

confidence: 98%

A model of the transient kinetics of laccase-catalyzed oxidation of phenol at micromolar concentrations

Rangelov

Nicell

2015

Biochemical Engineering Journal

View full text Add to dashboard Cite

“…51 For instance, whether it would be beneficial to run a reaction in a batch reactor, a packed bed reactor, or a continuous stirred tank reactor 52 or likewise evaluating opportunities for in situ product removal. 53,54 It can also be used for optimisation of reaction conditions, for instance finding the optimal ratio of enzyme and/or cosubstrate concentrations to provide the highest product yield. 55,56 For example, Zňidarsǐc-Plazl and Plazl 57 modelled the continuous extraction of steroids using ethyl acetate in a microsystem and achieved good agreement between model calculations and experimental data.…”

Section: ■ Process Modelsmentioning

confidence: 99%

Advances in the Process Development of Biocatalytic Processes

Tufvesson

Ramos

Al-Haque

et al. 2013

Org. Process Res. Dev.

View full text Add to dashboard Cite

Biocatalysis is already established in chemical synthesis on an industrial scale, in particular in the pharmaceutical sector. However, the wider implementation of biocatalysis is currently hindered by the extensive effort required to develop a competitive process. In order that resources spent on development are used in the most efficient manner for these challenging systems, a holistic view on process development and a more in-depth understanding of the underlying constraints (process related as well as biocatalyst related) are required. In this concept article a systematic approach to solve this problem is proposed, involving the use of process tools and methods to assist in development.

show abstract

Modelling as a tool of enzyme reaction engineering for enzyme reactor development

Abstract: Strategy of the development of model for enzyme reactor at laboratory scale with respect to the modelling of kinetics is presented. The recent literature on the mathematic modelling on enzyme reaction rate is emphasized.

Cited by 44 publications

References 63 publications

2‐Deoxyribose‐5‐phosphate aldolase from Thermotoga maritima in the synthesis of a statin side‐chain precursor: characterization, modeling and optimization

2‐Deoxyribose‐5‐phosphate aldolase from Thermotoga maritima in the synthesis of a statin side‐chain precursor: characterization, modeling and optimization

A model of the transient kinetics of laccase-catalyzed oxidation of phenol at micromolar concentrations

Advances in the Process Development of Biocatalytic Processes

Contact Info

Product

Resources

About