While increased tourist demand has brought economic benefits to tourist destinations, it has also put tremendous pressure on tourism-related ecological environments. Therefore, the relationship between regional urban tourism’s economic benefits and tourism ecologies must be considered by increasing the ecological security levels of regional urban tourism and promoting sustainable tourism development. This paper defines the ecological security of regional urban tourism, constructs an indicator system of said ecological security based on a pressure-state-response social-economic-environment (PSR-SEE) model, uses the entropy method to objectively assign weights to the indicator system, constructs a dynamic multi-scenario simulation model to assess ecological security under continuous, economic priority and various ecological protection scenarios, employs ArcGIS software to analyze the spatial distribution patterns of the ecological security of regional urban tourism, and uses a standard deviation ellipse method to analyze the space–time evolutionary track of the ecological security of regional urban tourism. The index system is applied to 16 cities in Hubei Province to measure the ecological security levels and test the viability of the system. The present research focuses on the space–time evolution of the ecological security of regional urban tourism, addresses the contradictions between socioeconomic development and the ecological security of regional urban tourism, provides an important reference for tourism destination planning, and can assist in improving the levels of the ecological security of regional urban tourism to promote sustainable tourism development.