Modelo experimental do carcinossarcoma 256 de Walker em bexiga de ratos

Dornelas, Conceição Aparecida; Almeida, Paulo Roberto Carvalho de; Nascimento, Germana Lopes do; Lima, Érika Bastos; Moraes, Manoel Odoríco de

doi:10.1590/s0102-86502006000100009

Cited by 4 publications

(1 citation statement)

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“…Without previous acid acetic inoculation the lesion is inconsistent and uneven, with tumor growth on few rats. That agrees with Oliveira et al 7 fi ndings where the inoculation on gastric mucosa was only made possible with a previous lesion and Dornelas et al 10 fi ndings where tumor implantation happened only on the spot where the bladder was injured, demonstrating that unharmed mucosa does not allow tumor implantation. On both groups B and C the tumor implantation rate was 100%, without difference between concentrations, similar to other inoculation Walker 256 carcinoma models, like kidney, 9 bladder, 10 stomach, 7 mouth 11 and others.…”

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confidence: 86%

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Brito

Carvalho

et al. 2007

Acta Cir. Bras.

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Purpose:To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. Methods: Fifteen female rats were used, and assigned to three groups each one with fi ve rats: group A -rats with 4x10 6 cells of Walker 256 carcinoma without acid acetic inoculation; group B -rats with 2x10 6 cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10 6 cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance -ANOVA. P values < 0.05 were taken to indicate statistical signifi cance. Results: Implantation and growth on GB and GC was 100% and on GA 20%. There was no statistical difference between GB and GC averages. Conclusion: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100% when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10 6 or 4x10 6 cells on its inoculation. Key words: Carcinoma 256, Walker. Cervix uteri. Vagina. Disease models, animal. Rats. RESUMOObjetivo: Estabelecer um modelo de inoculação de Tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 15 ratas fêmeas, virgens, adultas, pesando entre 200-250g, distribuídas em três grupos de estudo com cinco animais cada: grupo A (GA): ratas com tumor de Walker 256 em concentração de 4x10 6 sem ácido acético; grupo B (GB): ratas com tumor de Walker 256 em concentração de 2x10 6 células com ácido acético; grupo C (GC): ratas com tumor de Walker 256 em concentração de 4x10 6 células com ácido acético. No dia anterior à inoculação do tumor, foi realizada a inoculação de 0,3 ml de ácido acético a 10% na vagina das ratas de GB e GC; no dia seguinte, tanto estas como as ratas do grupo GA foram anestesiadas, feita a escarifi cação da parede vaginal com uma escova de endocérvice e inoculado 0,3ml de tumor na concentração de 4x10 6 células nos grupos GA e GC e 2x10 6 células no grupo GB. Após 12 dias, foi realizada a eutanásia e removido o tumor em bloco com vagina e cornos uterinos para análise, sendo pesado e averiguado seu volume e calculado as relaçõe...

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Section: Discussionsupporting

confidence: 86%

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Brito

Carvalho

et al. 2007

Acta Cir. Bras.

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Avaliação dos efeitos do azul de metileno fotoativado no modelo experimental do Tumor de Walker 256.

Petrellis¹

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Aos meus pais e familiares pelo estímulo, confiança e apoio recebido ao longo da minha vida.Ao Prof. Dr. Rodrigo Álvaro Brandão Lopes Martins, pela oportunidade, confiança e por me aceitar como aluna de doutorado. Aos professoresDr.Durvanei Augusto Maria do Instituto Butantan e Dra. Marília Cerqueira Leite Seelaender do Departamento de Biologia Celular e do Desenvolvimento do ICB, pelo apoio científico e experiência em pesquisas com câncer durante a realização deste trabalho. A Simone Aparecida Teixeira e Rosangela Aparecida dos Santos Eichler pelo apoio e auxílio técnico em análises de biologia molecular recebido durante a realização dos experimentos. A toda e equipe do laboratório, em especial Patrícia de Almeida, pela amizade, carinho, apoio, compreensão, paciência, incentivo e convivência durante este período. Aos amigos Rodney Capp Pallotta e Rodrigo Labat, pela amizade, paciência e ajuda científica recebida durante a realização deste trabalho. Agradeço aos demais professores do Departamento de Farmacologia, em especial ao Prof. Dr. Marcelo Nícolas Muscará e Profa. Dra. Maria Helena Catelli de Carvalho, pela oportunidade e confiança na realização dos experimentos em seus laboratórios. A todos os funcionários do Departamento de Farmacologia do ICB, em especial a Mônica Nunes e Camila Gonçalves Trindade, pela ajuda e orientação nos prazos e datas durante o doutorado. Agradeço a Capes pela conceção da bolsa de doutorado no Departamento de Farmacologia do ICB -USP. "A mente que se abre a uma nova idéia jamais voltará ao seu tamanho original". Albert Einstein Palavras-chave: Terapia Fotodiâmica. Laser. Agentes Fotosensibilizantes. Azul de Metileno. Câncer.Tumor de Walker 256.

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Efficacy of Intrathecal Morphine in a Model of Surgical Pain in Rats

et al. 2016

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Concerns over interactions between analgesics and experimental outcomes are a major reason for withholding opioids from rats undergoing surgical procedures. Only a fraction of morphine injected intravenously reaches receptors responsible for analgesia in the central nervous system. Intrathecal administration of morphine may represent a way to provide rats with analgesia while minimizing the amount of morphine injected. This study aimed to assess whether morphine injected intrathecally via direct lumbar puncture provides sufficient analgesia to rats exposed to acute surgical pain (caudal laparotomy).In an initial blinded, randomised study, pain-free rats received morphine subcutaneously (MSC, 3mg.kg-1, N = 6), intrathecally (MIT, 0.2mg.kg-1, N = 6); NaCl subcutaneously (NSC, N = 6) or intrathecally (NIT, N = 6). Previously validated pain behaviours, activity and Rat Grimace Scale (RGS) scores were recorded at baseline, 1, 2, 4 and 8h post-injection. Morphine-treated rats had similar behaviours to NaCl rats, but their RGS scores were significantly different over time and between treatments. In a second blinded study, rats (N = 28) were randomly allocated to one of the following four treatments (N = 7): MSC, 3mg.kg-1, surgery; MIT, 0.2mg.kg-1, surgery; NIT, surgery; NSC, sham surgery. Composite Pain Behaviours (CPB) and RGS were recorded as previously. CPB in MIT and MSC groups were not significantly different to NSC group. MSC and MIT rats displayed significantly lower RGS scores than NIT rats at 1 and 8h postoperatively. RGS scores for MIT and MSC rats were not significantly different at 1, 2, and 8h postoperatively. Intraclass correlation value amongst operators involved in RGS scoring (N = 9) was 0.913 for total RGS score. Intrathecal morphine was mostly indistinguishable from its subcutaneous counterpart, providing pain relief lasting up to 8 hours in a rat model of surgical pain. Further studies are warranted to clarify the relevance of the rat grimace scale for assessing pain in rats that have received opioid analgesics.

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Modelo experimental do carcinossarcoma 256 de Walker em bexiga de ratos

Cited by 4 publications

References 11 publications

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Avaliação dos efeitos do azul de metileno fotoativado no modelo experimental do Tumor de Walker 256.

Efficacy of Intrathecal Morphine in a Model of Surgical Pain in Rats

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