Moderate halophilic bacteria colonizing the phylloplane of halophytes of the subfamily Salicornioideae (Amaranthaceae)

Mora-Ruiz, Merit del Rocío; Font-Verdera, Francisca; Díaz‐Gil, Carlos; Urdiain, Mercedes; Rodríguez-Valdecantos, Gustavo; González, Bernardo; Orfila, Alejandro; Rosselló‐Móra, Ramon

doi:10.1016/j.syapm.2015.05.004

Cited by 53 publications

(39 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OTUs were clustered into OPUs as recommended by Mora‐Ruiz et al . (). Rarefaction curves and statistical indices were calculated using the PAST statistical tool (Hammer et al ., ), and divergence between samples was estimated based on phylogenetic distances between corresponding OPUs using non‐parametric Kolmogorov–Smirnov tests (Jarek, ).…”

Section: Summary Of the Methodsmentioning

confidence: 99%

Predominance of deterministic microbial community dynamics in salterns exposed to different light intensities

Viver

Orellana

Díaz

et al. 2019

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Summary While the dynamics of microbial community assembly driven by environmental perturbations have been extensively studied, our understanding is far from complete, particularly for light‐induced perturbations. Extremely halophilic communities thriving in coastal solar salterns are mainly influenced by two environmental factors—salt concentrations and high sunlight irradiation. By experimentally manipulating light intensity through the application of shading, we showed that light acts as a deterministic factor that ultimately drives the establishment of recurrent microbial communities under near‐saturation salt concentrations. In particular, the stable and highly change‐resistant communities that established under high‐light intensities were dominated (>90% of metagenomic reads) by Haloquadratum spp. and Salinibacter spp. On the other hand, under 37‐fold lower light intensity, different, less stable and change‐resistant communities were established, mainly dominated by yet unclassified haloarchaea and relatively diverse photosynthetic microorganisms. These communities harboured, in general, much lower carotenoid pigment content than their high‐irradiation counterparts. Both assemblage types appeared to be highly resilient, re‐establishing when favourable conditions returned after perturbation (i.e. high‐irradiation for the former communities and low‐irradiation for the latter ones). Overall, our results revealed that stochastic processes were of limited significance to explain these patterns.

show abstract

Section: Summary Of the Methodsmentioning

confidence: 99%

Predominance of deterministic microbial community dynamics in salterns exposed to different light intensities

Viver

Orellana

Díaz

et al. 2019

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…A second short PCR (five cycles) was performed in a final volume of 25 l in triplicate to incorporate tags and linker into the amplicon using 1:25 dilution of the original products as templates, and also using the same protocol that for the first PCR, primers GM3-PS and a variant of 907-PS (Supplementary Table S1). The PCR products were purified and sequenced as previously published [27]. The set of sequences has been deposited at the ENA sequence repository under the study project accession number PRJEB8518.…”

Section: Microbial Dna Extraction Pcr Amplification and Pyrosequencingmentioning

confidence: 99%

“…Sequences were aligned with the SINA aligner [37], using LTPs115 database [54]. The OPU design by phylogenetic inference was performed as previously described [27,50].…”

Section: Sequence Trimming Operational Taxonomic Unit (Otu) Clusterimentioning

confidence: 99%

“…The gastric cavity is tightly connected with the surrounding waters, and thus a source of microorganisms' exchange that may constitute an effective dispersal mechanism, and a source of biological indicators. To this purpose, we combined high quality pyrosequencing (long nucleotide sequences and large amounts of reads; [27]) with culturing of aerobic heterotrophs to reveal the microbial composition of the gastric cavity. The identification approach was based on the recognition of the Operational Phylogenetic Units (OPUs) that rely on phylogenetic inferences rather than sequence identity clustering, to better reflect the diversity observed [27,50].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prokaryotic microbiota in the digestive cavity of the jellyfish Cotylorhiza tuberculata

Cortés-Lara

Urdiain

Mora-Ruiz

et al. 2015

Systematic and Applied Microbiology

Self Cite

View full text Add to dashboard Cite

“…The taxonomic structure of bacterial communities in plant endophytic compartments is dominated by the phyla Proteobacteria and Actinobacteria Carrell & Frank, 2015;Mora-Ruiz et al, 2015;Yu et al, 2015). Firmicutes, Bacteroidetes, Gemmatimonadetes, Verrucomicrobia, Planctomycetes, Fusobacteria and some the other bacterial phyla may also be present but in lower abundances Sessitsch et al, 2012).…”

Section: Biodiversity Of Endophytic Bacteriamentioning

confidence: 99%

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

Liu¹

View full text Add to dashboard Cite

The main aim of this thesis was to characterise microbial properties of microbiomes associated with wheat (Triticum aestivum) soils and wheat plants. In particular my objective was to better understand the effects on microbial communities following strategic tillage (ST) in wheat field soils and the activation of plant defence pathways in wheat plants. Throughout the thesis, multiple culture-independent methods, especially next-generation sequencing of 16S rRNA gene were used to profile soil microbial communities. Over half of the arable land in the northern grains region of Australia is managed using no-till (NT), a farming method which has improved crop yields and soil quality while reducing the input and labour costs. However, in recent years, concerns have arisen among farmers over the weed control in continuous NT systems. Strategic tillage has been touted as a potential solution, in particular for the severe weed infestations of long-term NT. Nevertheless, there is little information on the influence of ST on the microbial properties of Australian NT soils.In the present study, ST applications and soil sampling were performed in Moonie, Moree and Condamine during the fallow period in eastern Australia. These sites were chosen based on their long history of repeated wheat cultivation under NT farming practices and their different soil types.Overall, results show that in the Moonie trial on a Calcisol, one-time ST with either chisel or offset disc did not significantly influence the composition of soil bacterial communities when measured 13 months after tillage. However, relative to the NT, chisel tillage led to significant increases in microbial biomass carbon (MBC), abundances of Alphaproteobacteria, Bacteroidetes and Firmicutes as well as the utilisation of D+cellubiose and mannitol at 0-10 cm depth. In the Moree trial on a grey Vertosol, ST with different timing and implement demonstrated great potential in weed control and did not influence wheat yield and soil physicochemical and biological properties in the short-term. In the Condamine site on a Solonetz soil, one-or two-time chisel tillage did not influence soil MBC, total microbial enzymatic activity (MEA) or utilisation of C substrates.Likewise, ST did not change the soil microbial community structure and the abundance of genes encoding enzymes involved in key steps of C and N reactions. However, one-time chisel increased relative abundance of Acidobacteria RB41 and Acidobacteria iii1-15, and two-time chisel slightly increased the average C utilisation, at 10-20 cm depth. Overall, this thesis suggests that ST does not cause major impacts on soil properties of long-term NT and could be potentially used to address the long-term NT-associated issues without impacting overall soil properties.Recent reports demonstrate the importance of microbiomes associated with plants and their soil they are cultivated in. Beneficial microbes can significantly increase crop yields and provide biocontrol functions against plant pathogens, but prior to this stu...

show abstract

Moderate halophilic bacteria colonizing the phylloplane of halophytes of the subfamily Salicornioideae (Amaranthaceae)

Cited by 53 publications

References 64 publications

Predominance of deterministic microbial community dynamics in salterns exposed to different light intensities

Predominance of deterministic microbial community dynamics in salterns exposed to different light intensities

Prokaryotic microbiota in the digestive cavity of the jellyfish Cotylorhiza tuberculata

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

Contact Info

Product

Resources

About