The present study aimed to investigate the effect of cold plasma on the developed bio-nanocomposite film. A dielectric barrier discharge system was utilized to generate the cold plasma. The films were treated under three different times (5, 10 and 15 min) and the characteristics of the films were evaluated. Results showed that the mechanical properties (tensile strength and elongation at break), water vapor permeability, oxygen transmission rate, moisture content and water contact angle characteristics were improved by cold plasma treatment. In addition, the cold plasma treatment influence the thermal stability and crystallization of the films. Also, the shelf life of the strawberries which were packed in the treated films under modified atmosphere condition was assessed. The results revealed that the mechanical properties (firmness and Young's modulus), chemical attributes (pH, soluble solid content and total ascorbic acid), physical characteristics (weight loss and color features), microbial activities (bacteria. yeast and mould) of the samples were affected by the treated films. As a result, cold plasma modification can be applied as an effective method to maintain and preservation of fresh fruit.