2013
DOI: 10.1371/journal.pone.0065218
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Modification of Glycosylation Mediates the Invasive Properties of Murine Hepatocarcinoma Cell Lines to Lymph Nodes

Abstract: Among the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of glycosylation mediated the invasive properties of Hca-F and Hca-P murine hepatocarcinoma cell lines, which have high, low metastatic potential in the lymph nodes, respectively. Analysis revealed that the N-glycan co… Show more

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Cited by 22 publications
(22 citation statements)
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“…In agreement with this corollary hypothesis, we find here that despite the enhancement of MGAT5 expression in EMT in GE11 cells (data not shown), silencing of St6gal1 almost completely inhibited the increased migratory ability upon TGF-␤ stimulation. Furthermore, this idea is further supported by a recent study (56) indicating that ST6GAL1 is responsible for the different invasive properties in the murine hepatocarcinoma Hca-F (high metastatic potential) and Hca-P (low metastatic potential) cells, although both St6gal1 and Gnt-V are highly expressed in Hca-F cells as compared with in Hca-P cells.…”
Section: Discussionsupporting
confidence: 57%
“…In agreement with this corollary hypothesis, we find here that despite the enhancement of MGAT5 expression in EMT in GE11 cells (data not shown), silencing of St6gal1 almost completely inhibited the increased migratory ability upon TGF-␤ stimulation. Furthermore, this idea is further supported by a recent study (56) indicating that ST6GAL1 is responsible for the different invasive properties in the murine hepatocarcinoma Hca-F (high metastatic potential) and Hca-P (low metastatic potential) cells, although both St6gal1 and Gnt-V are highly expressed in Hca-F cells as compared with in Hca-P cells.…”
Section: Discussionsupporting
confidence: 57%
“…Through the use of MS technology, thousands of new formerly N-linked glycopeptides from different tissues, cells, and bodily fluids have been identified and quantified (29,30). Zhang et al have used MALDI-TOF-MS to investigate novel N-glycan changes involved in lymphatic metastasis in the murine HCC cell lines Hca-F and Hca-P with high and low metastatic potential in the lymph nodes (31). To identify sialylated N-glycans associated with the metastatic potential of human HCC, we used an MS method and analyzed the composition profiling of N-glycans.…”
Section: Discussionmentioning
confidence: 99%
“…ST6GAL1 is expressed ubiquitously and catalyzes the ␣-2,6-sialylation of aterminal Gal residues of Gal␤1-4GlcNAc disaccharide, and high ST6Gal1 levels are associated with increased metastasis and poor patient prognosis (34 -36). Our previous report indicated that the remodeled levels of ST6GAL1 mediated the invasive properties of murine HCC cell lines to lymph nodes (31). ST8SIA2 encodes Nacetylgalactosaminide ␣-2,8-sialyltransferase II (ST8Sia II), which catalyzes the polycondensation of ␣-2,8-linked sialic acid required for the synthesis of polysialic acid and is a modulator of the adhesive properties of neural cell adhesion molecule.…”
Section: Discussionmentioning
confidence: 99%
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“…Modulation of glycan expression, particularly with regard to changes in asparagine-linked oligosaccharides, has been shown to be essential for cellular transformations in colorectal cancer (CRC) [39,60]. Moreover, among the various posttranslational modifications, glycosylation is reported to be the most common, and approximately half of all known proteins are glycosylated [61]. Knockdown of the first N-glycosylation gene, ALG7, in two genetically distinct haploid yeast cells resulted in an unexpected phenotype of mitochondrial dysfunction, indicating promise for future drug development [62].…”
Section: Glycan Biosynthesis and Metabolismmentioning
confidence: 99%