Modification of lily polysaccharide by selenylation and the immune-enhancing activity

Hou, Ranran; Chen, Jin; Yue, Chanjuan; Li, Xiuping; Liu, Jie; Gao, Zhenzhen; Liu, Cui; Lu, Yu; Wang, Deyun; Li, Hongquan; Hu, Yuanliang

doi:10.1016/j.carbpol.2016.01.032

Cited by 95 publications

(36 citation statements)

References 11 publications

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“…AMP was extracted by water decoction and ethanol precipitation, purified by trichloroacetic acid method to eliminate protein, and eluted with distilled water through DEAE cellulose-52 column. Nine sAMPs, sAMP 1 -sAMP 9 , were obtained by nitric acid-sodium selenite method on the basis of L 9 (3 4 ) orthogonal design of three factors (dosage of Na 2 SeO 3 , reaction time, and temperature), respectively, at three levels, 500 mg of AMP reacting with Na 2 SeO 3 at 200, 300, and 400 mg under 50, 70, and 90 ∘ C for 6, 8, and 10 h, respectively, according to our in-house protocol published before [12,13]. The modification conditions, yield, carbohydrate content, and selenium content were listed in 0.25, 0.125, 0.0625 mg/mL) in each group was added with 2 mL ABTS and reacted for 6 min at room temperature, and absorbance at 734 nm by microliter enzyme-linked immunosorbent assay reader was measured, PBS instead of polysaccharide as control group.…”

Section: Preparation Of Amp and Sampmentioning

confidence: 99%

Selenylation Modification of Atractylodes macrocephala Polysaccharide and Evaluation of Antioxidant Activity

Hou

Liu

et al. 2019

Advances in Polymer Technology

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The Atractylodes macrocephala polysaccharide (AMP) was extracted by water extracting-alcohol precipitating method and further purified by DEAE column. After that, the polysaccharides were modified by nitric acid-sodium selenite method, and nine kinds of selenizing AMPs (sAMPs) were obtained, namely, from sAMP1 to sAMP9. AMP and sAMP were characterized using FTIR spectrometry. Then their antioxidant activities in vitro were measured by free radical-scavenging test. Among these, sAMP6 presented the strongest antioxidant effect. For the test in vivo, the chickens at day 14 vaccinated with ND vaccine were repeatedly vaccinated at day 28. The chickens in sAMP and AMP were injected respectively with 1 mg of sAMP6 and AMP and, in vaccination control (VC) and BC groups, injected with equal volume of normal saline. Respectively, after the first vaccine, on days 7, 14, 21, and 28, the serum GSH-Px and SOD activities and MDA content were determined. The results suggested that sAMP6 could significantly promote GSH-Px and SOD activities and decrease MDA content. All these results indicated that selenylation modification could significantly enhance the antioxidant activity of AMP.

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Section: Preparation Of Amp and Sampmentioning

confidence: 99%

Selenylation Modification of Atractylodes macrocephala Polysaccharide and Evaluation of Antioxidant Activity

Hou

Liu

et al. 2019

Advances in Polymer Technology

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“…Therefore, immune-stimulants have been used as an adjuvant to gain long-lasting humoral and cellular immune responses and enhancement the response of vaccines. Strengthening of specific immunity (humoral and cell-mediated) will maximise the animal's ability to fight diseases (Hou et al, 2016;Yu, Shi, & Hu, 2015).…”

Section: Introductionmentioning

confidence: 99%

The ameliorating effect of some immune-stimulants on bursal and tracheal lesions of broiler chickens challenged with ND virus

Ali

Hafez

Mohamed

2019

JoBAZ

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Background: Nowadays, immuno-stimulants have great importance in improving the immune system and minimising the effect of infectious diseases in poultry flocks. This study was designed to demonstrate the impact of using different immunestimulants on the reduction of the harmful implications of virulent ND virus through evaluation of histopathological lesion scores on bursa and trachea of vaccinated and infected broiler chickens with Newcastle disease (ND). Results: Eighty Ross breed broiler chicks at the age of 1 day were randomly divided into four groups, each group formed of 20 birds. Group I was vaccinated against AI, ND and IBD and used as a control. Groups II, III and IV were immunised as group I and also had immuno-stimulant supplement, Imutrix® (oregano oils plus ß-glucan), Evit liquid® (Vitamin E plus selenium) and Immunair 17.5 (Propionibacterium acnes and E. coli lipopolysaccharides), respectively. All groups were experimentally infected with virulent ND virus at the age of 28 days. The morbidity rates for groups I, II, III and IV were 15%, 5%, 5% and 10% respectively. Histopathological examination of bursa revealed hyperplasia of lymphoid follicles in group I which became more evident in groups II, III and IV. Group III showed hyperplasia of tracheal epithelium with lymphocytes and heterophils infiltration in lamina propria. CD79A-positive cells were significantly higher in bursa of group III in comparison to other groups during an immunohistochemical examination. Conclusion: It was concluded that Evit liquid® (vitamin E plus selenium) supplementation might have potent immunomodulatory effect in chickens.

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“…This enhancement can be achieved by using of adjuvants and/or immunostimulant supplements. Therefore, immunostimulants have been used as adjuvants to give long lasting humoral and cellular immune responses and improving the response of vaccines [6,7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Some Immunostimulants on the Immune-response of Broiler Chickens Against Avian Influenza and Newcastle Diseases Vaccination

Abdelhafez

Mohamed

2016

Zagazig Veterinary Journal

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Immunostimulants get an increasing importance to enhance the immune system and allowing maximum performance in poultry production. The effects of using different immunostimulants on the immune response of broiler chickens to Newcastle disease (ND) and Avian influenza (AI) vaccination were investigated in this study. 100 day-old Ross broiler chicks were randomly divided into 4 groups, 25 birds for each group. Group A was not treated with any immunostimulants and used as a control, while groups B, C and D were supplemented with Imutrix ® (oregano oils plus β-glucan), Evit liquid ® (Vitamin E plus selenium) and inmunair ® 17.5 (Propionibacterium acnes and E. coli lipopolysaccharides), respectively. Statistical analysis results demonstrated significant divergence (P < 0.05) in body weight (BW) and feed conversion ratio among the applicable treatments. Birds of group B had the heaviest body weight (2.03 kg), followed by group C (1.87 Kg) and D (1.82 Kg) in comparison to the birds of group A (control, 1.79 Kg). Bursa of Fabricius weight showed non-significant differences among groups except in group B that showed an increase in the Bursa of Fabricius weight more than other groups especially the control group (1.15 g more). Comparable immune response was recorded for both AI and ND vaccination. The highest mean antibody titers were recorded for birds in group B at 14, 28 and 35 days old, while those in group D had the lowest antibody titre values. Thus, supplementing immunostimulants especially Imutrix ® and Evit liquid ® had a significant positive effect on performance characteristics and immune response against AI and ND vaccination in broiler chickens in contrast to non-treated group, which had low levels of immune response, rapid decreasing and no persistence for keeping high HI titer for long time.

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Modification of lily polysaccharide by selenylation and the immune-enhancing activity

Cited by 95 publications

References 11 publications

Selenylation Modification of Atractylodes macrocephala Polysaccharide and Evaluation of Antioxidant Activity

Selenylation Modification of Atractylodes macrocephala Polysaccharide and Evaluation of Antioxidant Activity

The ameliorating effect of some immune-stimulants on bursal and tracheal lesions of broiler chickens challenged with ND virus

Evaluation of Some Immunostimulants on the Immune-response of Broiler Chickens Against Avian Influenza and Newcastle Diseases Vaccination

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