Modifications in gonadotropin control and reproductive behavior in the female rat by hypothalamic and preoptic lesions

Nance, Dwight M.; Christensen, L. W.; Shryne, James E.; Gorski, Roger A.

doi:10.1016/0361-9230(77)90087-9

Cited by 64 publications

(21 citation statements)

References 26 publications

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“…MPO plays a gender-dependent role in reproductive behavior. In females, bilateral lesions of the MPO facilitate the occurrence of lordosis (Powers and Valenstein, 1972;Nance et al, 1977;Rodriguez-Sierra and Terasawa, 1979;Lisk and MacGregor, 1982;Hoshina et al, 1994); chemical or electrical stimulation of MPO suppresses it (Moss et al, 1974;Zasorin et al, 1975;Pfaff and Sakuma, 1979;Takeo et al, 1993). By contrast, in males MPO lesions completely abolish mounting, intromission, and ejaculation, whereas stimulation of this region induces copulation (Heimer and Larrson, 1966;Lisk, 1966;Malsbury et al, 1981;Hansen et al, 1982;Arendash and Gorski, 1983;Edwards and Einhorn, 1986).…”

Section: Reproductivementioning

confidence: 99%

Medial preoptic area afferents to periaqueductal gray medullo-output neurons: a combined Fos and tract tracing study

et al. 1996

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We have shown recently that the medial preoptic area (MPO) robustly innervates discrete columns along the rostrocaudal axis of the midbrain periaqueductal gray (PAG). However, the location of PAG neurons responsive to MPO activation is not known. Anterograde tract tracing was used in combination with Fos immunohistochemistry to characterize the MPO -+ PAG pathway anatomically and ,functionally within the same animal. Focal electrical or chemical stimulation of MPO in anesthetized rats induced extensive Fos expression within the PAG compared with sham controls. Fos-positive neurons were organized as 2-3 longitudinal columns. The organization and location of these columns overlapped remarkably well with the distribution of fibers and terminals in PAG labeled by Phaseolus vulgaris leucoagglutinin (PHA-L) injected into the same MPO stimulation site. This indicates that MPO inputs may terminate on the soma or proximal dendrites of neurons exhibiting elevated Fos. A second series of experiments investigated whether MPO stimulation excited PAG neurons with descending projections to the medulla. Retrograde labeling of PAG neurons projecting to the medial and lateral regions of the rostroventral medulla (RVM) was combined with MPO-induced Fos expression. The results showed that a substantial population (3743%)of Fos-positive PAG neurons projected to the ventral medulla. This indicates that MPO stimulation engages PAG-medullary output neurons. Taken together, these results suggest that the MPO + PAG + RVM projection constitutes a functional pathway. This circuit may coordinately regulate neuroendocrine, motor, and autonomic adjustments necessary for the elaboration of sexual behaviors.Key words: reproduction; antinociception; cardiovascular regulation; brainstem; sexual behavior; immunohistochemistryThe medial preoptic area (h/[PO) is a sexually dimorphic structure Simerly et al., 1984;Bloch and Gorski, 1988) that plays a pivotal role in sexual behavior and neuroendocrine function (Lisk, 1966;Powers and Valenstein, 1972;Pfaff and Sakuma, 1979;Hansen et al., 1982;Arendash and Gorski, 1983;Kalra and Kalra, 1983;Docke et al., 1984;Sachs and Meisel, 1988;Simerly et al., 1990;DonCarlos et al., 1991;Takeo et al., 1993;Hoshina et al., 1994). We recently reported that MPO robustly innervates the midbrain periaqueductal gray (PAG) (Rizvi et al., 1992) and terminates in discrete, longitudinally organized columns running through the rostrocaudal axis of PAG.PAG plays a key role in antinociception (Reynolds, 1969;Liebeskind et al., 1973;Oliveras et al., 1974;Behbehani and Fields, 1979; Lovick, 3985;Morgan and Liebeskind, 1987;Reichling et al., 1988;Lovick, 1990), cardiovascular control (Lovick, 1985;Carrive et al., 1987;Carrive et al., 1988;Carrive et al., 1989a;Lovick, 1990;Verberne and Guyenet, 1992;Murphy et al., 1994a;Murphy et al., 1995), and many of the same neuroendocrine and reproductive functions as MPO (Pfaff and SchwartzGiblin, 1988;Ogawa et al., 1991;Shipley et al., 1995). PAG neurons involved in these functions also are ...

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Section: Reproductivementioning

confidence: 99%

Medial preoptic area afferents to periaqueductal gray medullo-output neurons: a combined Fos and tract tracing study

et al. 1996

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“…Lesion experiments indicate that the medial POA and ZI are sites of inhibitory control of female sexual behaviour (41,42) and GABA may exert a tonic inhibitory effect in these areas with steroids stimulating sexual receptivity by reducing GABA synthesis. The potentiating effect of P o n stimulating sexual behaviour in OB-primed rats may arise as a consequence of additionally ARC/ME and ZI may be a necessary prerequisite for the positive feedback effect of steroids on LH release.…”

Section: Ospmentioning

confidence: 99%

Ovarian Steroid Regulation of Glutamic Acid Decarboxylase Gene Expression in Individual Hypothalamic Nuclei

Leigh

Carter

Horton

et al. 1990

J Neuroendocrinology

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The possibility that steroids exert their effects on luteinizing hormone (LH) and prolactin release and female sexual behaviour via altering 7-arninobutyric acid (GABA) synthesis within the hypothalamus was investigated. Ovariectomized rats were treated with e$ther oil, 5 pg oestradiol benzoate (OB) or 5 pg OB plus 0.5 mg progesterone (P) 48 h later; autopsy was carried out 54 h after the OB injection. At this time, both steroid treatments stimulated prolactin release and lordotic activity. OB alone exerted a negative feedback effect on LH release while OB plus P stimulated an LH surge.The brains of the rats in these three treatment groups were microdissected and glutamic acid decarboxylase messenger ribonucleic acid (GAD mRNA) was estimated in specific hypothalamic areas obtained from individual brains; these areas included the preoptic area (POA), ventromedial nucleus (VMN), anterior medial portion of the zona incerta (ZI) and the arcuatehedian eminence area (ARC/ME). GAD mRNA concentrations were assessed by the slot-blotting technique and expressed as a ratio of GAD mRNA: b-actin mRNA.Both steroid treatments significantly reduced GAD mRNA in the ARCIME and ZI and OB plus P also reduced the concentration in the POA, while OB alone had no effect in this area. Neither treatment affected GAD mRNA in the VMN. These results indicate that when steroids stimulate LH and prolactin release and female sexual behaviour, they reduce GABA activity probably by reducing GABA synthesis in the POA, ZI and ARCIME. This suggests that GABA normally has an inhibitory influence on these parameters. G4BA synthesis does not however, appear to be involved in the negative feedback effects of steroids on LH release as measured 54 h after OB treatment.;,-,iminobutyric acid (GABA) is widely distributed in the hypothalmius with its highest concentrations in the preoptic area (POA) aiid ventromedial nucleus (VMN) (1 -3). GABA-containing neurons possess steroid receptors (4, 5 ) and it has becn suggested that sri:roids might exert some of their CNS effects via GABAergic s! stems (6-9). These effects include the control of luteinizing hormone (LH) and prolactin release and sexual behaviour. Rep x t s in the literature indicate that GABA can influence sexual bchaviour in females (10, 11) and has a dual effect on the release of I €1 and prolactin (12, 13). The results show that GABA is inhibitory to LH release in the POA and anterior medial portion o!' the zona incerta (ZI) (14-17). The site of it's stimulatory effect is not really known, although some data indicate that it may be in the medial basdl hypothalamus (12,18,19). Within the hypothalaIlIus GABA stimulates prolactin release, but it is inhibitory at the level of the pituitary (12)(13)(14)20). There are many reports investigating the inter-relationship of the gonadal steroids and GABA, in particular noting the effect of oestrogen and progesterone (P) on GABA activity in specific brain areas. Since GABA is present in both neuronal and glial tissue (1, 21) changes in its concentra...

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“…A number of their axons inervate the preoptic area (POA), the septum or the amygdala (Parent et al, 1981). On the other hand, there is evidence indicating that these preoptic and limbic areas exert an inhibitory influence on the display of lordosis, because destruction of the POA (Powers & Valenstein, 1972;Nance et al, 1977, Rodoriguez-Sierra & Terasawa, 1979, septum (Nance et al, 1975) or lateral amygdala (Masco & Carrer, 1980) and transection of the dorsal afferent fibers at the level of the anterior commissure (Yamanouchi & Arai, 1977) potentiated the display of lordosis in females. In the present study, in order to elucidate correlation between serotonergic neurons and the forebrain lordosis inhibiting system, the effects of p-chloroamphetamin (PCA, a serotonin agonist) on lordosis response were examined in estrogen-progesterone primed ovariectomized rats, in which the inhibitory influence was removed by POA lesion or dorsal deafferentation of the POA.…”

Section: Possible Participationmentioning

confidence: 99%

Forebrain lordosis inhibiting system and serotonin neuron in female rats: Effect of P-chloroamphetamine.

Yamanouchi¹,

Watanabe

Okada

et al. 1982

Endocrinol Japon

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Relationship between forebrain lordosis inhibiting system and serotonergic mechanism was examined in estradiol benzoate (EB) and progesterone (P)-primed ovariectomized rats. In order to remove the forebrain inhibition of lordosis response, anterior roof deafferentation (ARD) which interrupts the dorsal inputs to the POA coming down anterior to the anterior commissure or medial preoptic area lesions (POA-L) were made. All experimental females pretreated with EB and P showed high levels of lordotic activity. Soon after the behavioral tests, the animals were injected with 10 mg/kg p-chloroamphetamine (PCA) intraperitoneally and tested again 30 min later. PCA strongly suppressed female sexual behavior.Regardless of the presence of ARD or POA-L, no PCA injected females showed lordosis. From these results, it can be said that serotonin may not directly act on the forebrain lordosis inhibiting system.

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Modifications in gonadotropin control and reproductive behavior in the female rat by hypothalamic and preoptic lesions

Cited by 64 publications

References 26 publications

Medial preoptic area afferents to periaqueductal gray medullo-output neurons: a combined Fos and tract tracing study

Medial preoptic area afferents to periaqueductal gray medullo-output neurons: a combined Fos and tract tracing study

Ovarian Steroid Regulation of Glutamic Acid Decarboxylase Gene Expression in Individual Hypothalamic Nuclei

Forebrain lordosis inhibiting system and serotonin neuron in female rats: Effect of P-chloroamphetamine.

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