Modifications to polyamines provoked by short-chain saturated fatty acids in Cicer arietinum seeds

Abstract

Abstract--Short-chain saturated fatty acids (SCSFA), ranging from pentanoic (C5) to decanoic acid (C~0), when added at micromolar concentrations to the germination medium of chick-pea seeds, did not significantly affect radicle emergence, although fresh weight diminished. Only heptanoic acid (C7) and octanoic acid (Cs) stimulated ethylene production and free spermidine and spermine content, whereas putrescine reached a maximum with nonanoic acid (C9). All the SCSFA tested increased the polyamines (PAs) bound to substances of low Mr; however, only hexanoic acid (C6) and C9 acid induced a strong appearance of PAs bound to substances of high Mr. Proline synthesis was stimulated in the presence of C7 acid and C9 acid, but values lower than control were obtained with the other SCSFA studied. (C 1997 Elsevier Science Ltd. All rights reserved INTRODUCTION

Tissue-specific expression of olive S-adenosyl methionine decarboxylase and spermidine synthase genes and polyamine metabolism during flower opening and early fruit development

“…Free and conjugated-Put, Spd and Spm were isolated as in Gomez-Jimenez et al (1997) with modifications. Frozen plant materials (about 0.2 g per samples) were powdered in the presence of liquid N 2 and extracted with a mixture of 0.2 N perchloric acid (4 mL g -1 FW) and 1,6-hexanediamine (1 lmol/g FW) as an internal standard.…”

“…An aliquot (100 lL) of the fractions thus obtained [i.e. supernatant (free, S), hydrolyzed supernatant (acid-soluble conjugated, SH) and hydrolyzed precipitate (acid-insoluble conjugated, PH)] were dansylated as described by Gomez-Jimenez et al (1997), before HLPC measurements were made. Experiments were repeated at least three times with three replicate measurements each.…”

Mature fruit abscission is associated with up-regulation of polyamine metabolism in the olive abscission zone

“…PAs were analyzed by high-performance liquid chromatography (HPLC) separation of dansyl chloride-derived PAs, as previously described (Gomez-Jimenez et al, 1997). These analyses were performed in triplicate in three independent experiments during the 2007-2008 growing seasons.…”