Modifications to the composition of the hyphal outer layer of Aspergillus fumigatus modulates HUVEC proteins related to inflammatory and stress responses

Neves, Gabriela W. P.; Curty, Nathália; Kubitschek-Barreira, Paula H.; Fontaine, Thierry; Souza, Gustavo H. M. F.; Cunha, Marcel Lyra; Goldman, Gustavo H.; Beauvais, Anne; Latgé, Jean‐Paul; Lópes-Bezerra, Leila M.

doi:10.1016/j.jprot.2016.06.015

Cited by 10 publications

(7 citation statements)

References 96 publications

(113 reference statements)

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“…Then, migrating polymorphonuclear neutrophils enter the stage and, in turn, participate in the elimination of conidia and filaments 23 . Nonetheless, previous studies also described the involvement of non-professional phagocytes, such as platelets, endothelial cells 24 , and epithelial cells 4 , 5 in the antifungal host response. For example, Perkhofer et al .…”

Section: Discussionmentioning

confidence: 99%

Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition

Richard

Marti

Varrot

et al. 2018

Sci Rep

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Aspergillus fumigatus is an environmental filamentous fungus that may act as an opportunistic pathogen causing a variety of diseases, including asthma or allergic bronchopulmonary aspergillosis, and infection, ranging from asymptomatic colonization to invasive pulmonary form, especially in immunocompromised patients. This fungus is characterized by different morphotypes including conidia which are the infective propagules able to germinate into hyphae. Due to their small size (2–3 µm), conidia released in the air can reach the lower respiratory tract. The objective of this study was to characterize the interactions between conidia and bronchial epithelial cells. To this end, we studied the role of bronchial epithelial cells, i.e., the BEAS-2B cell line and human primary cells, in conidial germination of a laboratory strain and three clinical strains of A. fumigatus. Microscopic observations and galactomannan measurements demonstrated that contact between epithelial cells and conidia leads to the inhibition of conidia germination. We demonstrated that this fungistatic process is not associated with the release of any soluble components nor internalization by the epithelial cells. We highlight that this antifungal process involves the phosphoinositide 3-kinase pathway on the host cellular side and the lectin FleA on the fungal side. Collectively, our results show that bronchial epithelial cells attenuate fungal virulence by inhibiting germination of extracellular conidia, thus preventing the morphological change from conidia to filaments, which is responsible for tissue invasion.

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Section: Discussionmentioning

confidence: 99%

Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition

Richard

Marti

Varrot

et al. 2018

Sci Rep

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“…The Ku80 pyrG + parental strain (WT) [2] , the UGM1 mutant ( Δugm1 ) and the reconstituted strain ( Δugm1:ugm1 ) [3] were cultivated as previously described [1] , [4] . The purified urea-soluble fraction of galactosaminogalactan (GAG) was obtained as described [5] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…HUVECs were obtained as previously described in [1] , [7] . The use of HUVECs was approved through the Research Ethics Committee of the Municipal Health Secretary and Civil Defense of Rio de Janeiro (CEP SMSDC-RJ), protocol no.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…For the interaction assays, HUVEC monolayers were infected with germlings of A. fumigatus WT and ∆ ugm1 strains, and with purified GAG (1 µg/mL), as described previously [1] . Uninfected HUVEC monolayers (control) were maintained in the same culture conditions.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Protein identification and quantitative data packaging were performed as previously described [1] , [12] , [13] , [14] , [15] . A cut-off Log2ratio values of +1.0 (2-fold) or higher was applied for determining the proteins with higher abundance levels and −1.0 (2-fold) or lower for proteins with lower abundance levels to compare pairs of experimental groups [16] , [17] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Dataset of differentially regulated proteins in HUVECs challenged with wild type and UGM1 mutant Aspergillus fumigatus strains

et al. 2016

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Invasive aspergillosis is the primary opportunistic invasive fungal infection described in neutropenic hematologic patients, caused by the angioinvasive pathogen Aspergillus fumigatus. The molecular mechanisms associated with A. fumigatus infection in the vascular endothelium are poorly understood. In this context, we used a high-throughput proteomic approach to unveil the proteins modulated in HUVECs after interaction with a wild type strain and the UGM1 mutant (Δugm1) of A. fumigatus. The proteomic analysis was also performed in HUVECs challenged with a galactosaminogalactan (GAG) purified from A. fumigatus cell wall. The dataset presented here correspond to all proteins identified that fit a 2-fold change criteria (log 2 ratio ≥ 1 or ≤ −1), disregarding the statistical validation cut off, in order to supplement the research article entitled “Modifications to the composition of the hyphal outer layer of Aspergillus fumigatus modulates the HUVEC proteins associated with inflammatory and stress responses” (G.W.P. Neves, N.A. Curty, P.H. Kubitschek-Barreira, T. Fontaine, G.H.M.F. Souza, M. Lyra Cunha, G.H. Goldman, A. Beauvais, J.P. Latgé, L.M. Lopes-Bezerra, 2016) [1]. The mass spectrometry proteomic data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002823.

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Proteomics and Its Application to the Human Pathogenic Fungus Aspergillus fumigatus

Krüger,

Kniemeyer

2024

The Mycota

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Modifications to the composition of the hyphal outer layer of Aspergillus fumigatus modulates HUVEC proteins related to inflammatory and stress responses

Cited by 10 publications

References 96 publications

Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition

Human Bronchial Epithelial Cells Inhibit Aspergillus fumigatus Germination of Extracellular Conidia via FleA Recognition

Dataset of differentially regulated proteins in HUVECs challenged with wild type and UGM1 mutant Aspergillus fumigatus strains

Proteomics and Its Application to the Human Pathogenic Fungus Aspergillus fumigatus

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