Modified acid-PAGE method for rapid screening and phenotyping of wheat gliadin mutant lines

Watry, Hannah; Zerkle, Alexander; Laudencia‐Chingcuanco, Debbie

doi:10.1016/j.mex.2020.100858

Cited by 5 publications

(5 citation statements)

References 22 publications

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“…A-PAGE resolved the different gliadin fractions (α, β, γ, ω-1.2 and ω-5; Fig. 1A) very well, as expected, 19 also highlighting subtle qualitative and quantitative differences among different wheat varieties (Fig. 1A).…”

Section: Biochemical Analysis Of Native and Transamidated Gliadinssupporting

confidence: 76%

“…In particular, we used A-PAGE to analyse isopeptide bond formation in the different gliadin fractions. 19 Transamidation was associated with a dramatic change in the electrophoretic mobility for all gliadin fractions. In particular, kinetic analysis of the reaction showed the ω-gliadins to be transamidated more rapidly, but that, after one hour, all of the different fractions were completely modified.…”

Section: Food and Function Papermentioning

confidence: 96%

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Transamidated wheat gliadin induces differential antigen recognition in the small intestine of HLA/DQ8 transgenic mice

et al. 2022

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Section: Biochemical Analysis Of Native and Transamidated Gliadinssupporting

confidence: 76%

Section: Food and Function Papermentioning

confidence: 96%

Transamidated wheat gliadin induces differential antigen recognition in the small intestine of HLA/DQ8 transgenic mice

et al. 2022

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“…Over the decades, scientists have found that the use of electrophoresis of the wheat storage protein, gliadin, is one of the methods that make it possible to distinguish cultivars from each other (Autran et al, 1979;Watry et al, 2020). Differences in gliadin spectra are associated with the presence of allelic diversity of genes localized at the main loci: Gli-A1, Gli-B1, Gli-D1, Gli-A2, Gli-B2, Gli-D2.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the genetic diversity of the alleles of gliadin-coding loci in common wheat (<i>Triticum aestivum</i> L.) collections in Kazakhstan and Russia

Utebayev,

Dashkevich,

Kradetskaya

et al. 2024

Vestn. VOGiS

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The study of genetic resources using prolamin polymorphism in wheat cultivars from countries with different climatic conditions makes it possible to identify and trace the preference for the selection of the alleles of gliadine-coding loci characteristic of specific conditions. The aim of the study was to determine the “gliadin profile” of the collection of common wheat (Triticum aestivum L.) from breeding centers in Russia and Kazakhstan by studying the genetic diversity of allelic variants of gliadin-coding loci. Intrapopulation (μ ± Sμ) and genetic (H) diversity, the proportion of rare alleles (h ± Sh), identity criterion (I) and genetic similarity (r) of common wheat from eight breeding centers in Russia and Kazakhstan have been calculated. It has been ascertained that the samples of common wheat bred in Kostanay region (Karabalyk Agricultural Experimental Station, Kazakhstan) and Chelyabinsk region (Chelyabinsk Research Institute of Agriculture, Russia) had the highest intrapopulation diversity of gliadin alleles. The proportion of rare alleles (h) at Gli-B1 and Gli-D1 loci was the highest in the wheat cultivars bred by the Federal Center of Agriculture Research of the South-East Region (Saratov region, Russia), which is explained by a high frequency of occurrence of Gli-B1e (86 %) and Gli-D1a (89.9 %) alleles. Based on identity criterion (I), the studied samples of common wheat from different regions of Kazakhstan and Russia have differences in gliadincoding loci. The highest value of I = 619.0 was found when comparing wheat samples originated from Kostanay and Saratov regions, and the lowest I = 114.4, for wheat cultivars from Tyumen and Chelyabinsk regions. Some region-specific gliadin alleles in wheat samples have been identified. A combination of Gli-A1f, Gli-B1e and Gli-Da alleles has been identified in the majority of wheat samples from Kazakhstan and Russia. Alleles (Gli-A1f, Gli-A1i, Gli-A1m, Gli-A1o, Gli-B1e, Gli-D1a, Gli-D1f, Gli-A2q, Gli-B2o, and Gli-D2a) turned out to be characteristic and were found with varying frequency in wheat cultivars in eight regions of Russia and Kazakhstan. The highest intravarietal polymorphism (51.1 %) was observed in wheat cultivars bred in Omsk region (Russia) and the lowest (16.6 %), in Pavlodar region (Kazakhstan). On the basis of the allele frequencies, a “gliadin profile” of wheat from various regions and breeding institutions of Russia and Kazakhstan was compiled, which can be used for the selection of parent pairs in the breeding process, the control of cultivars during reproduction, as well as for assessing varietal purity.

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“…For decades, scientists authenticated that wheat seed storage protein (gliadin) can be used to distinguish cultivars from one another (Autran et al, 1979;Metakovsky, 1991;Watry et al, 2020). Differences in gliadin spectra are associated with the allelic diversity localized in To cite this manuscript: Utebayev MU, Dolinny YY, Dashkevich SM, Bome NA (2022).…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, gliadin blocks may differ in intensity, electrophoretic mobility, and molecular weight of the components Poperelya, 1980: Obukhova andShumny, 2016). The use of buffer mixtures containing lactic (Bushuk and Zillman, 1978, Metakovsky et al, 1984: Branlard et al, 1990Utebayev et al, 2019;Watry et al, 2020), formic (Kozub et al, 2009), and acetic (Clements, 1988;Konarev et al, 2005) acids determines the total gliadin by electrophoresis on a polyacrylamide gel.…”

Section: Introductionmentioning

confidence: 99%

Allelic Composition of Gliadin-Coding Loci as a 'Portrait' in Spring Soft Wheat Selections of Russian and Kazakh Origins

UTEBAYEV¹,

DOLINNY²,

DASHKEVICH³

et al. 2022

SABRAOJBG

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The progressive study determined the gliadin 'profile' and genetic diversity based on allelic variations of gliadin-coding loci in spring soft wheat (Triticum aestivum L.) selection from Russian and Kazakh origins. A total of 139 samples obtained from a spring soft wheat collection consisting of 62 from Russia and 77 from Kazakhstan were studied. As a result of electrophoretic analysis in Russian wheat, the share of monomorphic gliadin cultivars was 63% and polymorphic was 37%. However, in the Kazakh wheat collection, the share of polymorphic gliadin cultivars was 26%. The alleles were mostly found among the Russian cultivars, i.e., Gli-А1f (46.9%), Gli-В1е (43.7%), Gli-В1b (30.8%), Gli-D1а (61.0%), Gli-А2l (17.0%), Gli-А2m (16.9%), Gli-В2o (16.1%), and Gli-D2q (19.8%). In wheat genotypes collected from Kazakhstan, the following alleles dominated, i.e., Gli-А1f (47.4%), Gli-В1е (72.0%), Gli-D1а (61.7%), Gli-А2l (25.3%), Gli-А2s (16.2%), Gli-В2r (25.7%), and Gli-D2a (40.3%). The study compiled the so-called 'ideal' electrophoretic spectrum of gliadin for several countries to visualize the 'portrait' of wheat, created based on common blocks of gliadin identified by the researchers at different times. It assumed that cultivars close to the 'ideal' spectrum in gliadin alleles should have a complex of economically valuable features. For example, the spectrum of Russian wheat consists of the blocks of components controlled by alleles, i.e., f, e, a, q, o, e. As for the Kazakh wheat, its 'ideal' spectrum (f, e, a, l, r, a) coincides with the spectrum of Russian wheat at the loci Gli-1, as Russian cultivars were often taken as parental genotypes by the Kazakhstan breeders.

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Modified acid-PAGE method for rapid screening and phenotyping of wheat gliadin mutant lines

Cited by 5 publications

References 22 publications

Transamidated wheat gliadin induces differential antigen recognition in the small intestine of HLA/DQ8 transgenic mice

Transamidated wheat gliadin induces differential antigen recognition in the small intestine of HLA/DQ8 transgenic mice

Assessment of the genetic diversity of the alleles of gliadin-coding loci in common wheat (<i>Triticum aestivum</i> L.) collections in Kazakhstan and Russia

Allelic Composition of Gliadin-Coding Loci as a 'Portrait' in Spring Soft Wheat Selections of Russian and Kazakh Origins

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