Modified Carbon Nanotubes Decorated with ZIFs as New Immobilized Metal Ion Affinity Chromatography Platform for Enrichment of Phosphopeptides

Abstract

ZIF‐8, as one of the most representative zeolite imidazolate frameworks, is rarely used for the enrichment of phosphopeptides due to its dissolution in acid solution. By introducing branched‐chain polyethyleneimine (PEI), we have overcome the acid‐intolerance shortcoming of ZIF‐8 and applied it for phosphopeptides enrichment. A material with magnetic carbon nanotube (mCNT) as substrate, polydopamine (PDA) as hydrophilic interlayer and PEI‐ZIF‐8 as functional groups (denoted as mCNT@PDA@PEI‐ZIF‐8) was prepared through a facile method. The obtained material exhibited excellent performance on phosphopeptides enrichment, including high sensitivity (0.5 fmol), outstanding selectivity (molar ratio of β‐casein: BSA=1 : 2000), reusability of at least 6 times and so on. The enrichment performance on complicated biological samples was also remarkable, 20 phosphopeptides were successfully detected from human saliva and 4 phosphopeptides from human serum, exhibiting its great potential and bright prospects in phosphopeptides enrichment.

Cobalt Phthalocyanine-Modified Magnetic Metal–Organic Frameworks for Specific Enrichment of Phosphopeptides

“…Detailed comparison of enrichment performance with other reported MOF-based materials is listed in Table . ,,,− The comparison consisted of sensitivity, selectivity, recovery, and biological application. It was clear that Fe 3 O 4 @MIL-101-CoPc showed better performance in aspects of selectivity and recovery for the enrichment of low-abundance phosphopeptides.…”

Effective Enrichment of Phosphopeptides Using Magnetic Polyoxometalate-Based Metal–Organic Frameworks

“…In addition, compared with other MOF materials, Fe 3 O 4 -POMOFs exhibited better enrichment performance for phosphopeptides (Table ). − …”

Effects of Carbon Nanomaterials and Aloe vera on Melanomas—Where Are We? Recent Updates

“…In addition, carbon nanomaterials have attracted attention in the areas of diagnostics and biomarkers, especially in the detection of phosphorylated proteins and peptides in biological samples. The characterization of phospholipids and phosphorylated proteins provides important information on physiological and pathological states associated with the removal or addition of phosphate groups, which control cellular functions [ 113 , 114 ]. However, phosphorylated biomolecules are labile, which poses a challenge in proteomic analyses.…”