Modified electrode with reduced graphene oxide/poly(3-hydroxyphenylacetic acid): a new platform for oligonucleotide hybridization

Silva, Jussara Vieira da; Madurro, Ana G. B.; Madurro, João M.

doi:10.1007/s10008-017-3601-8

Cited by 9 publications

(7 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Da Silva et al . , gold electrode/rGO showed good electrocatalytic activity in relation to the oxidation of the monomer, and a larger surface area for formation of Au/rGO/polymer composite film, when compared with the bare gold electrode.…”

Section: Resultsmentioning

confidence: 94%

See 1 more Smart Citation

Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

et al. 2019

View full text Add to dashboard Cite

This work describes an electrochemical genosensor for detection of genomic RNA of Zika virus in real samples of infected patients, using a new platform based on graphite electrodes modified with electrochemically reduced graphene oxide and polytyramine‐conducting polymer. The developed genosensor was suitable for differentiation between samples of healthy and infected patients with Zika virus by differential pulse voltammetry, detecting up to 0.1 fg/mL (1.72 copies/mL), showing good stability (about 60 days), rapid analysis (about 20 min) and potential for filling the lack of practical diagnostic methods for Zika virus.

show abstract

Section: Resultsmentioning

confidence: 94%

“…Graphene oxide (GO) was produced according to Da Silva et al . . Graphene oxide (GO) dispersion was prepared with deionized water (1 mg/mL) in ultrasonic bath for 20 min, stirred for 1 min and centrifuged at 3500 rpm for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

et al. 2019

View full text Add to dashboard Cite

show abstract

“…A preconditioning of the electrode was performed in a 0.5 M HClO 4 solution through cyclic voltammetry between 0 V and +1.2 V, 4 cycles, 50 mV/s, to clean it from any electroactive contaminants. Graphene oxide (GO) was produced according to the Hummers method with some modifications, as described in previous studies [24]. The GO was solubilized in water (1 mg/mL), sonicated for 20 min for its exfoliation and then centrifugated for 10 min at 1500 g. 30 μL of the supernatant were pipetted onto the GE.…”

Section: Methodsmentioning

confidence: 99%

A Novel and Reusable Electrochemical Genosensor for Detection of Beef Adulteration

et al. 2020

View full text Add to dashboard Cite

This work describes the construction of a genosensor based on a graphite electrode modified with an reduced graphene oxide/poly(3‐hidroxybenzoic acid) nanocomposite with an specific DNA oligonucleotide for detection of cattle mitochondrial DNA, in order to certify beef purity. Electrochemical and morphological analyses indicate that the genosensor allows duplex formation with the DNA of pure sample of beef lysate. The genosensor was selective, identifying up to 1 % (w/w) of pork in beef samples, showing good reproducibility and stability within six weeks of storage, and can be reused four times, being a great tool for the evaluation of beef purity, with application in the meat production and marketing chain.

show abstract

“…Graphite electrodes (GE) were mechanically polished with aqueous suspension of alumina (0.3 μm), washed with deionized water, dried and preconditioned in HClO 4 0.50 mol L À 1 solution by cyclic voltammetry from 0 V to + 0.8 V, 4 cycles, 50 mV s À 1 . After, 20 μL of the supernatant of a graphene oxide (GO) solution, prepared according to previous studies [21], were dripped onto the electrode and incubated at 55°C until drying. Electrochemical reduction of the GO was performed by cyclic voltammetry in sodium phosphate buffer solution (0.10 mol L À 1 , pH 7.4), between 0 V to À 1.5 V, 10 cycles, 50 mV s À 1 .…”

Section: Modification Of Graphite Electrode With Ergo/polytyraminementioning

confidence: 99%

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

et al. 2020

View full text Add to dashboard Cite

This work describes the preparation of a reusable electrochemical immunosensor for detection of C-reactive protein (CRP), based in modified carbon electrode with reduced graphene oxide and polytyramine as strategy for anchoring the anti-CRP probe. This immunosensor was able to distinguish different concentrations of CRP target in human serum, by differential pulse voltammetry, showing a linear response from 1.09 to 100 μg L À 1. The performance of the immunosensor allowed its application in four consecutive assays, without activity loss. Analyses by atomic force microscopy, scanning electron microscopy and electrochemical impedance spectroscopy corroborate with voltammetric analysis. The immunosensor showed selectivity, stability and reusability, being a promising platform for point-of-care quantification of CRP.

show abstract

Modified electrode with reduced graphene oxide/poly(3-hydroxyphenylacetic acid): a new platform for oligonucleotide hybridization

Cited by 9 publications

References 63 publications

Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

A Novel and Reusable Electrochemical Genosensor for Detection of Beef Adulteration

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

Contact Info

Product

Resources

About