Modified gellan gum hydrogels for tissue engineering applications

Ferris, Cameron J.; Gilmore, Kerry J.; Wallace, Gordon G.; Panhuis, Marc in het

doi:10.1039/c3sm27389j

Cited by 127 publications

(81 citation statements)

References 93 publications

(178 reference statements)

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“…GG hydrogels are considered biologically inert materials [17]. According to earlier studies, GG does not in vitro support neuronal cell migration on top of gels or as encapsulated without the addition of cell adhesion cues [18; 28].…”

Section: Page 28 Of 39 Author Submitted Manuscript -Bmm-101572r1mentioning

confidence: 99%

“…GG is a deacetylated form of gellan molecule which has a tetrasaccharide repeating structure of β-D-glucose, β-D-glucuronic acid and α-L-rhamnose in a 2:1:1 ratio [13]. Like many other polysaccharides, GG is a relatively inert biomaterial [17]. To improve cell attachment, GG-based hydrogels have been functionalized with peptides by covalently binding them in the molecule backbone itself [17; 18].…”

Section: Introductionmentioning

confidence: 99%

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Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

et al. 2017

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Neural tissue engineering and three-dimensional in vitro tissue modeling require the development of biomaterials that take into account the specified requirements of human neural cells and tissue. In this study, an alternative method of producing biomimetic hydrogels based on gellan gum (GG) was developed by replacing traditional crosslinking methods with the bioamines spermidine and spermine. These bioamines were proven to function as crosslinkers for GG hydrogel at +37 °C, allowing for the encapsulation of human neurons. We studied the mechanical and rheological properties of the formed hydrogels, which showed biomimicking properties comparable to naïve rabbit brain tissue under physiologically relevant stress and strain. Human pluripotent stem cell-derived neuronal cells demonstrated good cytocompatibility in the GG-based hydrogels. Moreover, functionalization of GG hydrogels with laminin resulted in cell type-specific behavior: neuronal cell maturation and neurite migration.

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Section: Page 28 Of 39 Author Submitted Manuscript -Bmm-101572r1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

et al. 2017

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“…Although the GG polysaccharide is cytocompatible, it does not participate in the specific cell binding interactions 69 . As a result, anchorage dependant cells grown in pure GG matrices may exhibit low levels of cell attachment or anomalous differentiation behaviours.…”

Section: Blending For Biofunctionalitymentioning

confidence: 99%

“…GG was recently highlighted for its significant capacity for tissue engineering applications, particularly after blending or chemical modification 69 . Here, we will discuss the numerous modifications and blends that have been employed to tailor the biological, mechanical, chemical and electrical functionality of GG.…”

Section: Blends Of Gellan Gummentioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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“…Dissolved in water above 40°C gellan gum adopts a random coil formation which when cooled to room temperature undergoes a transition from disordered-to-ordered forming helices. [19] The gel is produced via association of the helical structures forming junction zones with this helix-coil transition being reversible upon heating.…”

Section: Building Integrated Concentrating Pv 'Smart Window' Systemmentioning

confidence: 99%

Design and development of a reflective membrane for a novel Building Integrated Concentrating Photovoltaic (BICPV) ‘Smart Window’ system

Connelly

Chen

et al. 2016

Applied Energy

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. Specifically, a switching temperature (Ts) of ~42 °C (6 wt. % HPC) was recorded, the measured transmittance decreases from ~ 90% to ~20%, with the temperature of the reflective layer increasing from 20°C to 60°C. No hysteresis in optical property was observed upon heating-cooling cycle of HPC membrane samples. The measured reflectivity increased with heating from ~10 % below the Ts to ~50 % above the Ts (for 6 wt. % HPC). These features indicate that the as-prepared HPC based thermotropic hydrogel layer holds great potential for application in next generation BICPV smart windows.

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Modified gellan gum hydrogels for tissue engineering applications

Cited by 127 publications

References 93 publications

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

Tissue engineering with gellan gum

Design and development of a reflective membrane for a novel Building Integrated Concentrating Photovoltaic (BICPV) ‘Smart Window’ system

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