Modified gold nanoparticles for intracellular delivery of anti-liver cancer siRNA

Shaat, Hanan; Mostafa, Amany A.; Moustafa, Moustafa; Gamal‐Eldeen, Amira M.; Emam, Ahmed N.; El‐Hussieny, Enas A.; ElHefnawi, Mahmoud

doi:10.1016/j.ijpharm.2016.03.051

Cited by 58 publications

(30 citation statements)

References 38 publications

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“…Other than thiol-based interactions are less common amongst the RNA-metal nanoparticles conjugates. These are usually electrostatic interactions between positive MeNP surfaces and RNA [150][151][152][153][154][155][156]. DNA and RNA fragments bind to endosomal toll-like receptors (TLRs), involved in innate immune system activation [157].…”

Section: Rna-gold Nanoparticles (Aunp) Conjugatesmentioning

confidence: 99%

“…Dendronized AuNPs with cumulative positive charge are able to attract and hold negatively charged RNA fragments, cross cell membrane and effectively silence the target gene without significant cytotoxicity [152]. Similar approach was utilized in delivery of siRNA targeted against c-Myc oncogene [153]. In this scenario branched polyethyleneimine was used as an attachment block to electrostatically connect siRNA.…”

Section: Rna-gold Nanoparticles (Aunp) Conjugatesmentioning

confidence: 99%

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Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

et al. 2020

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Development of nanotechnology has become prominent in many fields, such as medicine, electronics, production of materials, and modern drugs. Nanomaterials and nanoparticles have gained recognition owing to the unique biochemical and physical properties. Considering cellular application, it is speculated that nanoparticles can transfer through cell membranes following different routes exclusively owing to their size (up to 100 nm) and surface functionalities. Nanoparticles have capacity to enter cells by themselves but also to carry other molecules through the lipid bilayer. This quality has been utilized in cellular delivery of substances like small chemical drugs or nucleic acids. Different nanoparticles including lipids, silica, and metal nanoparticles have been exploited in conjugation with nucleic acids. However, the noble metal nanoparticles create an alternative, out of which gold nanoparticles (AuNP) are the most common. The hybrids of DNA or RNA and metal nanoparticles can be employed for functional assemblies for variety of applications in medicine, diagnostics or nano-electronics by means of biomarkers, specific imaging probes, or gene expression regulatory function. In this review, we focus on the conjugates of gold nanoparticles and nucleic acids in the view of their potential application for cellular delivery and biomedicine. This review covers the current advances in the nanotechnology of DNA and RNA-AuNP conjugates and their potential applications. We emphasize the crucial role of metal nanoparticles in the nanotechnology of nucleic acids and explore the role of such conjugates in the biological systems. Finally, mechanisms guiding the process of cellular intake, essential for delivery of modern therapeutics, will be discussed.

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Section: Rna-gold Nanoparticles (Aunp) Conjugatesmentioning

confidence: 99%

Section: Rna-gold Nanoparticles (Aunp) Conjugatesmentioning

confidence: 99%

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

et al. 2020

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“…Conde et al developed a complex Au-nanoplatform and demonstrated in vitro and in vivo the efficacy of RNAi AuNP-based nanocarrier against the C-MYC proto-oncogene [46]. As well, Shaat et al designed a polyelectrolyte complex from AuNPs with a specific siRNA to target the C-MYC gene [47]. More recently, Cordeiro et al proved both the suitability and the great efficiency of Au-nanobeacons for gene silencing in embryos of a fli-enhanced green fluorescence protein (fli-EGFP) transgenic zebrafish line [48].…”

Section: Addressing Current Drawbacks In Gene Silencing Therapy—fomentioning

confidence: 99%

Gold Nanoparticle Approach to the Selective Delivery of Gene Silencing in Cancer—The Case for Combined Delivery?

Mendes

Fernandes

Baptista

2017

Genes

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Gene therapy arises as a great promise for cancer therapeutics due to its potential to silence genes involved in tumor development. In fact, there are some pivotal gene drivers that suffer critical alterations leading to cell transformation and ultimately to tumor growth. In this vein, gene silencing has been proposed as an active tool to selectively silence these molecular triggers of cancer, thus improving treatment. However, naked nucleic acid (DNA/RNA) sequences are reported to have a short lifetime in the body, promptly degraded by circulating enzymes, which in turn speed up elimination and decrease the therapeutic potential of these drugs. The use of nanoparticles for the effective delivery of these silencers to the specific target locations has allowed researchers to overcome this issue. Particularly, gold nanoparticles (AuNPs) have been used as attractive vehicles for the target-specific delivery of gene-silencing moieties, alone or in combination with other drugs. We shall discuss current trends in AuNP-based delivery of gene-silencing tools, considering the promising road ahead without overlooking existing concerns for their translation to clinics.

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“…[12] Furthermore, when combined with electrospun membranes and a drug delivery system, siRNA may lose its functional activity through the interaction with heat, organic solvent, and the oil/water interface. [14] In this context, both the need to protect siRNA during electrospinning and the low transfection efficiency of nanoparticles may limit their physiological potential. [14] In this context, both the need to protect siRNA during electrospinning and the low transfection efficiency of nanoparticles may limit their physiological potential.…”

Section: Introductionmentioning

confidence: 99%

Gene Silencing via PDA/ERK2‐siRNA‐Mediated Electrospun Fibers for Peritendinous Antiadhesion

Liu

et al. 2018

Advanced Science

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Sustained delivery of small interfering RNA (siRNA) is a challenge in gene silencing for managing gene‐related disorders. Although nanoparticle‐mediated electrospun fibers enable sustainable gene silencing, low efficiency, loss of biological activity, toxicity issues, and complex electrospinning techniques are all bottlenecks of these systems. Preventing peritendinous adhesion is crucial for their successful use, which involves blocking cellular signaling via physical barriers. Here, a multifunctional, yet structurally simple, cationic 2,6‐pyridinedicarboxaldehyde‐polyethylenimine (PDA)‐mediated extracellular signal‐regulated kinase (ERK)2‐siRNA polymeric delivery system is reported, in the form of peritendinous antiadhesion electrospun poly‐l‐lactic acid/hyaluronan membranes (P/H), with the ability to perform sustained release of bioactive siRNA for long‐term prevention of adhesions and ERK2 silencing. After 4 days of culture, the cell area and proliferation rate of chicken embryonic fibroblasts on siRNA+PDA+P/H membrane are significantly less than those on P/H and siRNA+P/H membranes. The in vivo results of average optical density of collagen type III (Col III) and gene expression of ERK2 and its downstream SMAD3 in the siRNA+PDA+P/H group are less than those of P/H and siRNA+P/H groups. Consequently, siRNA+PDA+P/H electrospun membrane can protect the bioactivity of ERK2‐siRNA and release it in a sustained manner. Moreover, adhesion formation is inhibited by reducing fibroblast proliferation and Col III deposition, and downregulating ERK2 and its downstream SMAD3.

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Modified gold nanoparticles for intracellular delivery of anti-liver cancer siRNA

Cited by 58 publications

References 38 publications

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

Gold Nanoparticle Approach to the Selective Delivery of Gene Silencing in Cancer—The Case for Combined Delivery?

Gene Silencing via PDA/ERK2‐siRNA‐Mediated Electrospun Fibers for Peritendinous Antiadhesion

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