Modified liquid–liquid interface cultivation system with floating microspheres and binder micro-pieces for slow-growing or unicellular microorganisms: Application to interfacial bioconversions with an actinomycete and yeasts

Oda, Shinobu; Nakanishi, Mami; Ishikawa, Asako; Baba, Toshiki

doi:10.1016/j.procbio.2019.02.003

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Citronellyl acetate is a colorless liquid with a lemon odor and is an important compound in flavor and fragrance [24]. Therefore, citronellyl acetate is widely used in the food, beverage, cosmetics, perfume, and pharmaceutical industries and has great application value [25]. The natural raw materials of citronellyl acetate are scarce, and the natural extraction method is not suitable for large-scale industrial production [26].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Pseudomonas fluorescens Lipase on Hollow Poly(o-phenylenediamine) Microspheres and Its Application in the Preparation of Citronellyl Acetate

Xiong

Wang

et al. 2023

Processes

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In order to address the challenges associated with free lipase in organic solvents, including aggregation, poor stability, and low catalytic efficiency, this study developed two types of poly(o-phenylenediamine) microspheres (solid and hollow) as supports for immobilizing lipase. The immobilization process utilized an adsorption method, with the poly(o-phenylenediamine) hollow microspheres being identified as the optimal support in a 2:5 enzyme-to-support ratio. On this basis, the lipase was immobilized by the covalent binding method. The immobilization conditions consisted of treating the support with 2% glutaraldehyde and immobilization at 40 °C for 2 h in pH 7.0 buffer. The specific activity of the immobilized enzyme was 5.3 times higher than that of the free enzyme. Covalent-binding immobilized lipase was also used for the preparation of citronellyl acetate by transesterification reaction, and, in optimized reaction conditions where the amount of immobilized enzyme was 0.1 g/mL, the reaction temperature was 50 °C and the shaking speed was 200 r/min during the reaction. Under these conditions, the citronellyl acetate yields can exceed 99% after 2 h. Furthermore, the stability of the immobilized lipase was investigated, and the residual activity of the immobilized enzyme was 95% after seven repetitions, while that of the free enzyme was only 70%. After 56 days of storage at room temperature, the immobilized enzyme retained 60% of its original viability, while the free enzyme retained only 31%.

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Section: Introductionmentioning

confidence: 99%

Immobilization of Pseudomonas fluorescens Lipase on Hollow Poly(o-phenylenediamine) Microspheres and Its Application in the Preparation of Citronellyl Acetate

Xiong

Wang

et al. 2023

Processes

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“…Citronellyl acetate is a terpene ester with special fragrance, found in volatile essential oil of magnolia, rose and citronella [1], which is widely modulated in the various perfumes with fragrances of rose, lily, lavender, carnation, apple and lemon, etc. [2], and also used in the production of medicine, food and cosmetics [3]. Thus, the demand for citronellyl acetate from market is large, but the natural citronellyl acetate directly extracted from rose or other plants by vacuum fractionation does not meet with the actual requirements in that the product gotten by extraction is low in yield and purity, and high in cost [4].…”

Section: Introductionmentioning

confidence: 99%

Rice straw enhancing catalysis of Pseudomonas fluorescens lipase for synthesis of citronellyl acetate

Yuan

Cong

Zhai

et al. 2021

Bioprocess Biosyst Eng

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Citronellyl acetate as an important flavor, can be effectively synthesized by lipase catalysis in nonaqueous system. But lipases usually behave low catalytic activity due to aggregation and denaturation of them in organic phase. To enhance the nonaqueous catalysis, based on the mechanism of lipases activated at water/oil (organic phase) interface, the inexpensive race straw was processed into powder and filaments on which Pseudomonas fluorescens lipase was immobilized by physical adsorption, used for synthesis of citronellyl acetate via transesterification of citronellol and vinyl acetate. Results showed that the desired loading was 10 mg lipase immobilized on 30 mg rice straw filaments or 25 mg rice straw powder. When the two immobilized lipases were employed in the reaction system consisted of 1-mL citronellol and 2-mL vinyl acetate at 37 ℃ and 160 rpm, the conversions all reached 99.8% after 12 h. Under the reaction condition, the conversion catalyzed by 10 mg native lipase was 85.1%. Undergoing six times of 8-h reuses in the organic system, the filament and power immobilized lipases had weak activity attenuation rates 0.36 and 0.32% h −1 , lower than 1.52% h −1 of native lipase. Even at the room temperature and the static state without shaking and stirring, the rice straw filaments immobilized lipase could brought conversion 62.9% after 10 h but the native lipase only gave 37.0%. Obviously, the rice straw, especially its filaments, is an inexpensive and available natural material to prepare immobilized lipase with desired catalysis in organic phase, meant significant potential in flavor industry.

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Microbial transformation of water-insoluble substrates by two types of novel interface bioprocesses, tacky liquid–liquid interface bioreactor and non-aqueous sporular bioconversion system

Oda

2020

World J Microbiol Biotechnol

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Modified liquid–liquid interface cultivation system with floating microspheres and binder micro-pieces for slow-growing or unicellular microorganisms: Application to interfacial bioconversions with an actinomycete and yeasts

Cited by 4 publications

References 39 publications

Immobilization of Pseudomonas fluorescens Lipase on Hollow Poly(o-phenylenediamine) Microspheres and Its Application in the Preparation of Citronellyl Acetate

Immobilization of Pseudomonas fluorescens Lipase on Hollow Poly(o-phenylenediamine) Microspheres and Its Application in the Preparation of Citronellyl Acetate

Rice straw enhancing catalysis of Pseudomonas fluorescens lipase for synthesis of citronellyl acetate

Microbial transformation of water-insoluble substrates by two types of novel interface bioprocesses, tacky liquid–liquid interface bioreactor and non-aqueous sporular bioconversion system

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