Modified Method of High Quality Genomic DNA Extraction from Mungbean [<i>Vigna radiata</i>(L.) Wilczek] Suitable for PCR Based Amplification

Dharajiya, Darshan T.; Khadia, Satish M.; Pagi, Nalin K.; Khatrani, Tarun; Jasani, Hitesh V.; Khunt, Akshay D.; Ravindrababu, Y.

doi:10.17485/ijst/2017/v10i20/95082

Cited by 9 publications

(5 citation statements)

References 26 publications

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“…Leaf samples from 21-day old mung beans were harvested, 100 mg per plant, frozen in liquid nitrogen and stored at −80 °C for DNA extraction. Total genomic DNA was extracted using CTAB method with slight improvements [22], and the quality determined by 1% agarose gel electrophoresis. It was then diluted to 20 ng/μl and stored at −20 °C until use.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Mwangi

Okoth

Kariuki

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Mung bean is a pulse crop principally grown in the tropic and subtropic parts of the world for its nutrient-rich seeds. Seven mung beans accessions from Eastern Kenya were evaluated using thirteen phenotypic traits. In addition, 10 SSR markers were used to determine their genetic diversity and population structure. This aimed at enhancing germplasm utilization for subsequent mung bean breeding programs. Results Analysis of variance for most of the phenology traits showed significant variation, with the yield traits recording the highest. The first three principal components (PC) explained 83.4% of the overall phenotypic variation, with the highest (PC1) being due to variation of majority of the traits studied such as pod length, plant height, and seeds per pod. The dendogram revealed that the improved genotypes had common ancestry with the local landraces. The seven mung beans were also genotyped using 10 microsatellite markers, eight of which showed clear and consistent amplification profiles with scorable polymorphisms in all the studied genotypes. Genetic diversity, allele number, and polymorphic information content (PIC) were determined using powermarker (version 3.25) and phylogenetic tree constructed using DARWIN version 6.0.12. Analysis of molecular variance (AMOVA) was calculated using GenALEx version 6.5. A total of 23 alleles were detected from the seven genotypes on all the chromosomes studied with an average of 2.875 across the loci. The PIC values ranged from 0.1224 (CEDG056) to 0.5918 (CEDG092) with a mean of 0.3724. Among the markers, CEDG092 was highly informative while the rest were reasonably informative except CEDG056, which was less informative. Gene diversity ranged from 0.1836 (CEDG050) to 0.5102 (CDED088) with an average of 0.3534. The Jaccards dissimilarity matrix indicated that genotypes VC614850 and N26 had the highest level of dissimilarity while VC637245 and N26 had lowest dissimilarity index. The phylogenetic tree grouped the genotypes into three clusters as revealed by population structure analysis (K = 3), with cluster III having one unique genotype (VC6137B) only. AMOVA indicated that the highest variation (99%) was between individual genotype. In addition, marker traits association analysis revealed 18 significant associations (P < 0.05). Conclusion These findings indicate sufficient variation among the studied genotypes that can be considered for germplasm breeding programs.

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Section: Molecular Characterizationmentioning

confidence: 99%

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Mwangi

Okoth

Kariuki

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…[12] It is a shrubby, perennial, xerophyte, succulent, pea-green color plant and contains 75 potentially active constituents: vitamins, enzymes, minerals, sugars, lignin, saponins, salicylic acids, and amino acids. [13] It is used as a green reducing agent for the synthesis of metal oxide with good optimum reduction efficiency. [14] Hence, the aim of our attempt was to synthesize and characterize the ZnO NPs using A. barbadensis Mill.…”

Section: Introductionmentioning

confidence: 99%

Biogenic Synthesis and Characterization of ZnO Nanoparticles from Aloe barbadensis Miller Leaf Extract

Jangannanavar

Patil

Chougala

et al. 2021

Macromolecular Symposia

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This study is aimed to synthesize zinc oxide nanoparticles (ZnO NPs) from Aloe barbadensis Miller leaf extract using the green synthesis method at room temperature. The biosynthesized NPs are characterized by X‐ray diffraction (XRD), UV–vis spectroscopy (UV–vis), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscope (SEM). The XRD pattern has revealed the hexagonal wurtzite structure with a crystallite size of 16 nm for synthesized ZnO NPs. The FTIR results display the formation of ZnO NPs and the presence of other functional groups. The UV–vis absorption spectrum shows an absorption band at 347 nm and the value of the bandgap is found to be 3.75 eV. The surface morphology of synthesized NPs is visualized by SEM images, it is observed that most of the NPs are needle in shape. Further, these NPs will find applications in biosensing and photocatalytic activity.

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“…Total DNA was isolated from mung bean leaves using a modified CTAB (hexadecyltrimethylammonium bromide) as described in Dharajiya et al (2017). NanoDrop (2000c Spectrometer, Thermo Scientific) was used to quantify DNA samples.…”

Section: Isolation and Quantitation Of Genomic Dnamentioning

confidence: 99%

Genetic evaluation of F2 and F3 interspecific hybrids of mung bean (Vigna radiata L. Wilczek) using retrotransposon‐based insertion polymorphism and sequence‐related amplified polymorphism markers

Fatmawati,

Ilyas,

Setiawan

et al. 2023

Indones. J. Biotechnol.

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Mung bean (Vigna radiata L. Wilczek) is a self‐pollinating and indispensable pulse crop in Indonesia. While low yield productivity is a major concern, genetic improvement is possible through interspecific hybridization. However, interspecific hybridization is relatively infrequent and produces low recombination exchanges, significantly limiting crop breeding efficiency. Thus, a comprehensive study is needed of the selection and genetic diversity evaluation of progenies in advanced generations derived from interspecific hybridization using a specific molecular marker. This study aims to confirm the heterozygosity in the F2 population and assess the genetic diversity in F3 mung bean populations resulting from interspecific hybridization between the mung bean and common bean. We designed the retrotransposon‐based insertion polymorphism (RBIP) marker by identifying the syntenic regions in the flanking sequences of retrotransposon insertion in common bean and mung bean. The RBIP marker can be applied to distinguish the heterozygote progenies from the homozygote progenies. Six combinations of sequence‐related amplified polymorphism (SRAP) primers were used in the genotyping of F3 mung bean progenies. The SRAP marker showed a high degree of polymorphism of up to 100%, while high genetic variation was observed within the population (71%) of mung bean progenies. The F3.4 population had the greatest number of genotypes and displayed the highest number of effective alleles, private alleles, and percentage of polymorphic loci, suggesting the existence of high genetic diversity within this population. These genetic diversity data are exceptionally critical for future genetic research since it has potentially high yield production. The genomic and marker‐assisted selection studies will support the major goals of the mung bean breeding program.

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Modified Method of High Quality Genomic DNA Extraction from Mungbean [Vigna radiata(L.) Wilczek] Suitable for PCR Based Amplification

Cited by 9 publications

References 26 publications

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Biogenic Synthesis and Characterization of ZnO Nanoparticles from Aloe barbadensis Miller Leaf Extract

Genetic evaluation of F2 and F3 interspecific hybrids of mung bean (Vigna radiata L. Wilczek) using retrotransposon‐based insertion polymorphism and sequence‐related amplified polymorphism markers

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