Modified pectin-based carrier for gene delivery: Cellular barriers in gene delivery course

Katav, Tali; Liu, LinShu; Traitel, Tamar; Goldbart, Riki; Wolfson, Marina; Kost, Joseph

doi:10.1016/j.jconrel.2008.06.002

Cited by 88 publications

(54 citation statements)

References 46 publications

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“…Modified cationic pectins were able to interact with DNA to form a compact transportable unit that is biocompatible and biodegradable, and demonstrated high transfection efficiency. Pectin's galactose residues contributes to DNA transfection, suggesting that modified pectin is a promising non-viral carrier for targeted gene delivery to cells with galactose-binding lectins on their surface [33].…”

Section: Pectin and Pectin/zeinmentioning

confidence: 99%

Hydrogels from Biopolymer Hybrid for Biomedical, Food, and Functional Food Applications

et al. 2012

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Hybrid hydrogels from biopolymers have been applied for various indications across a wide range of biomedical, pharmaceutical, and functional food industries. In particular, hybrid hydrogels synthesized from two biopolymers have attracted increasing attention. The inclusion of a second biopolymer strengthens the stability of resultant hydrogels and enriches its functionalities by bringing in new functional groups or optimizing the micro-environmental conditions for certain biological and biochemical processes. This article presents approaches that have been used by our groups to synthesize biopolymer hybrid hydrogels for effective uses for immunotherapy, tissue regeneration, food and functional food applications. The research has achieved some challenging results, such as stabilizing physical structure, increasing mucoadhesiveness, and the creation of an artificial extracellular matrix to aid in guiding tissue differentiation.

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Section: Pectin and Pectin/zeinmentioning

confidence: 99%

Hydrogels from Biopolymer Hybrid for Biomedical, Food, and Functional Food Applications

et al. 2012

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“…Hyperbranched cationic glycogen derivatives as efficient gene vectors the DMAPA-Glyp/pDNA and the AEPZ-Glyp/pDNA complexes, respectively (lanes [3][4][5][6][7][8][9][10][11][12][13][14]. This demonstrated that the glycogen derivatives exhibited distinct protective effects against DNase I.…”

mentioning

confidence: 86%

“…11 Following amination, various cationized polysaccharide derivatives have been designed and synthesized as gene-delivery vectors, with high transfection efficiency. 3,[11][12][13][14][15][16][17][18] Glycogen is a storage form of glucose that occurs predominantly in the liver and skeletal muscles of mammalian and shellfish species. 19 It is a natural hyperbranched polysaccharide with high weight-average molecular weight: about 10 6 -10 7 g/mol.…”

Section: Introductionmentioning

confidence: 99%

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Liu¹,

Ren²,

Liu³

et al. 2014

IJN

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Background The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector. Methods A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy. Their buffer capacity was assessed by acid–base titration in aqueous NaCl solution. Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis. The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy. Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively. pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines. In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency. Results The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized. They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI). They were able to bind and condense pDNA to form the complexes of 100–250 nm in size. The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI. Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group. Conclusion The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.

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“…Dentro da área farmacêutica vem sendo explorada para o desenvolvimento de novos sistemas de liberação de fármacos, devido às propriedades promissoras desse material como baixa toxicidade, biocompatibilidade, degradabilidade e baixo custo 60,61 . Outra vertente muito explorada é a utilização da pectina como excipiente na obtenção de sistemas de liberação cólon-específica de fármacos, uma vez que em meio ácido permanece como agregados de macromoléculas, além de ser resistente às proteases e às amilases presentes nas porções superiores do trato gastrintestinal (TGI), sendo degradada por enzimas produzidas pela microbiota colônica 44,50,57 .…”

Section: Pectinaunclassified

“…Recentemente, a pectina também tem sido investigada na vetorização de genes 50 . Além disso, surgiu um grande interesse na utilização da pectina na engenharia de tecidos, principalmente devido à semelhança química desse material com a matriz extracelular de tecidos dos mamíferos 54,70 .…”

Section: Pectinaunclassified

Biopolímeros: aplicações farmacêutica e biomédica

et al. 2016

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____________________________________________________________________________Resumo: O estudo e desenvolvimento de polímeros para aplicação nas áreas biomédica e farmacêutica têm aumentado devido às suas propriedades peculiares que contribuem para a melhoria da qualidade de vida, como por exemplo, os polímeros usados em medicina regenerativa e sistemas de liberação de fármacos. O desenvolvimento de novos materiais baseados em polímeros depende desde os métodos de síntese, extração ou composição desses materiais até os estudos da influência de suas estruturas e propriedades em aplicações específicas, entre outras. Esta revisão descreve o uso de alguns de polímeros novos e convencionais com potencial para aplicação nas áreas farmacêutica e biomédica, enfatizando as principais propriedades que os tornam aplicáveis. Palavras-chave: biopolímero, medicina regenerativa, liberação de fármacos ____________________________________________________________________________ Abstract:The study and development of polymers for pharmaceutical and biomedical use has been increasing due to their peculiar properties that contribute for the improvement of the life quality, such as the polymers used in regenerative medicine and in drug release systems. The development of new polymer based materials and its composites depends on several steps, such as the synthesis approach, the extraction, the composition, the influence of their properties on the specific applications, and others. This review describes the use of conventional and new polymers with potential application in pharmaceutical and biomedical fields, highlighting the properties that allow them to be useful for such purposes.

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Modified pectin-based carrier for gene delivery: Cellular barriers in gene delivery course

Cited by 88 publications

References 46 publications

Hydrogels from Biopolymer Hybrid for Biomedical, Food, and Functional Food Applications

Hydrogels from Biopolymer Hybrid for Biomedical, Food, and Functional Food Applications

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Biopolímeros: aplicações farmacêutica e biomédica

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