Modified protocol for culturing Drosophila S2 R+ cells and adult plasmatocytes to study actin cytoskeleton dynamics

Weaver, Ceileigh M.; Makdissi, Stephanie; Cara, Francesca Di

doi:10.1016/j.xpro.2022.101588

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…The efficacy and toxicity of antimicrobial compound screens can be measured in similar ways to determine their efficacy and toxicity ( 69 , 70 ). Examples of microscopy techniques include confocal microscopy for visualising phagocytosis in fungi-stimulated plasmatocytes ( 71 ), electron microscopy for imaging effects of treatment on host cell morphology ( 35 ) and fungal burden ( 72 ). Immunofluorescence staining and bioluminescence allow visualisation of individual cell types in tissue, larvae or adult flies, and can be done through RNA in situ hybridization ( 30 , 73 , 74 ), intravital 2-d photon microscopy and reporter systems (GFP, lacZ) ( 62 ).…”

Section: Application Of Drosophila To Human Fungal...mentioning

confidence: 99%

“…Infection progression can be measured through survival, microbial load, mRNA quantification, melanisation and microscopy assays (55,64). The efficacy and toxicity of antimicrobial compound screens can be measured in similar ways to determine their efficacy and toxicity (69,70). Examples of microscopy techniques include confocal microscopy for visualising phagocytosis in fungi-stimulated plasmatocytes (71), electron microscopy for imaging effects of treatment on host cell morphology (35) and fungal burden (72).…”

Section: Application Of Drosophila To Human Fungal Pathogens Tools Av...mentioning

confidence: 99%

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The utility of Drosophila melanogaster as a fungal infection model

Mpamhanga,

Kounatidis

2024

Front. Immunol.

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Invasive fungal diseases have profound effects upon human health and are on increase globally. The World Health Organization (WHO) in 2022 published the fungal priority list calling for improved public health interventions and advance research. Drosophila melanogaster presents an excellent model system to dissect host-pathogen interactions and has been proved valuable to study immunopathogenesis of fungal diseases. In this review we highlight the recent advances in fungal-Drosophila interplay with an emphasis on the recently published WHO’s fungal priority list and we focus on available tools and technologies.

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Section: Application Of Drosophila To Human Fungal...mentioning

confidence: 99%

Section: Application Of Drosophila To Human Fungal Pathogens Tools Av...mentioning

confidence: 99%

The utility of Drosophila melanogaster as a fungal infection model

Mpamhanga,

Kounatidis

2024

Front. Immunol.

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A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells

Mohati,

Matak,

Makdissi

et al. 2024

STAR Protocols

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Innervated Coculture Device to Model Peripheral Nerve-Mediated Fibroblast Activation

Cariba,

Srivastava,

Bronsema

et al. 2024

ACS Biomater. Sci. Eng.

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Cutaneous wound healing is a complex process involving various cellular and molecular interactions, resulting in the formation of a collagen-rich scar with imperfect function and morphology. Dermal fibroblasts are crucial to successful wound healing, migrating to the wound site where they are activated to provide extracellular matrix remodeling and wound closure. Peripheral nerves have been shown to play an important role in wound healing, with loss or damage to these nerves often leading to impaired healing and the formation of chronic nonhealing wounds. Previous research has suggested that sensory nerves secrete trophic factors that can regulate wound healing, including fibroblast activation; however, the direct cell−cell interaction between nerves and fibroblasts has not been extensively studied. To address this knowledge gap, we developed an in vitro co-culture model using a device called the IFlowPlate. This model supports the long-term viability of multiple cell types while allowing for direct contact between sensory nerve cells and dermal fibroblasts. Using the IFlowPlate, we demonstrate that co-culture of dorsal root ganglia with dermal fibroblasts increases fibroblast proliferation, collagen and α-smooth muscle actin expression, and secretion of pro-wound healing factors, suggesting that nerves can promote wound healing by modulating fibroblast activation. The IFlowPlate offers a userfriendly and high-throughput platform to study the in vitro interactions between nerves and a variety of cell types that can be applied to wound healing and other important biological processes.

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Modified protocol for culturing Drosophila S2 R+ cells and adult plasmatocytes to study actin cytoskeleton dynamics

Cited by 4 publications

References 9 publications

The utility of Drosophila melanogaster as a fungal infection model

The utility of Drosophila melanogaster as a fungal infection model

A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells

Innervated Coculture Device to Model Peripheral Nerve-Mediated Fibroblast Activation

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