Modified Silver Impregnation as a Method for Fish Nervous Tissue Visualization

Veretennikov, Nikolay; Shkil, F. N.

doi:10.1134/s0032945222030043

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…For the analysis of vessel development, Ehrlich's hematoxylin and eosin counterstain (H‐E) technique was used 32 . To evaluate the innervation of the pectoral fin, we applied silver impregnation according to the modified Romanes method, 33 adapted to fish samples and allowing staining of both myelinated and non‐myelinated nerve fibers 34 . Stained slides were dehydrated in a series of ethanol solutions (30%, 50%, 70%, and 100%), cleared in HistoChoice and mounted with the Vitrogel synthetic mounting media (BioVitrum, 12‐005).…”

Section: Methodsmentioning

confidence: 99%

Direct development of the catfish pectoral fin: An alternative pectoral fin pattern of teleosts

Shkil

Borisov

Veretennikov

et al. 2022

Developmental Dynamics

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Background The study of the teleosts' pectoral fin development touches on many crucial issues of evolutionary biology, from the formation of local adaptations to the tetrapod limbs' origin. Teleosts' pectoral fin is considered a rather developmentally and anatomically conservative structure. It displays larval and adult stages differing in the skeletal and soft tissues' composition. Larva‐adult transition proceeds under the thyroid hormone (TH) control that defines pectoral fin ontogeny as an indirect development. However, the outstanding diversity of teleosts allows suggesting the existence of lineage specific developmental patterns. Results We present a description of the North African catfish, Clarias gariepinus, pectoral fin development. It lacks a clear larval stage and directly develops the adult skeleton with the associated musculature and innervation. Interestingly, the development of catfish pectoral fin appears not to be under the TH dependence. Conclusion This catfish displays a direct pectoral fin developmental trajectory differing from the stereotyped teleost pattern. In the absence of the larval endoskeletal disk and TH control, the catfish's proximal radials arise in a manner somewhat similar to the metapterygial radials in basal actinopterygians and humerus in sarcopterygians. Thus, the catfish fin pattern seems homoplastic, arising by convergence with, or reversion to the ancestral developmental mechanisms.

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Section: Methodsmentioning

confidence: 99%