Modified Taohong Siwu decoction improves cardiac function after myocardial ischaemia and reperfusion in rats by promoting endogenous stem cell mobilization and regulating metabolites

Abstract

Context Taohong Siwu decoction (THSWD) has been shown to promote heart repair in myocardial infarction. Objective To determine the effects of modified THSWD (THSWD plus four ingredients) on myocardial ischaemia and reperfusion (I/R) injury. Materials and methods Sixty Sprague-Dawley rats were randomly divided into the I/R group and three different modified THSWD dose groups (gavage administration, 1.215, 2.43, and 4.86 g, respectively). 2,3,5-Triphenyltetrazolium chloride and Evans blue staining were used to detect the infarct area at 24 h after treatment. The serum biochemical indexes and cell apoptosis were examined to determine myocardial injury. The number of endogenous stem cells, expression of stromal dell derived factor-1 (SDF-1) and stem cell factor (SCF), and cardiac function were measured at 4 weeks. The serum was collected for metabolomic analysis. Results The high-dose modified THSWD group presented a reduced infarction area (decreased by 21.3%), decreased levels of lactate dehydrogenase and creatinine kinase, attenuated cell apoptosis, and enhanced superoxide dismutase activity in early stage I/R compared with other groups. The serum SCF and SDF-1 levels were higher in the high-dose group than in the I/R group. At 4 weeks, the infarct size and collagen content were the lowest, and the ejection fraction and fractional shortening values were the highest in the high-dose group. Moreover, high-dose modified THSWD affected the metabolism of phosphonate and phosphonate, taurine, and hypotaurine. Conclusions Endogenous stem cell mobilization and metabolic regulation were related to the cardioprotection of modified THSWD. We provided a new strategy and direction for the treatment of cardiovascular diseases with traditional Chinese medicine.

Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

“…MIRI model was conducted according to our previous study [ 30 ]. Brifely, after anesthetizing with isoflurane (Bio-Rad), chest cavity was opened at the 4th intercostal space under mechanical ventilation.…”

Mesenchymal Stem Cells and Their Exocytotic Vesicles

“…Differences in the angiogenic biological activity of MSCs are also associated with the expression of several genes involved in angiogenesis, such as HGF, angiopoietin-1 (ANGPT), or matricellular proteins (fibronectin (FN), insulin-like growth factor binding protein 7 (IGFBP7)) [113]. In addition, stromal cell-derived factor-1 (SDF-1) and stem cell factor (SCF) contributed to reducing infarct size and collagen content and protecting the heart [114]. MSCs secreted chemokine (C-C motif) ligand 7 (CCL7)/chemokine (C-C motif) receptor 1 (CCR1) to activate downstream CBP/P300 acetylation of KLF5 to promote CXC ligand 5 (CXCL5) transcription and secreted TGF-β to reverse the effect of KLF5 on transcriptional activation by regulating SMAD4, and CXCL5 promoted distant tumor metastasis and angiogenesis [115].…”